Bioinspired Iron Porphyrin Covalent Organic Frameworks-Based Nanozymes Sensor Array: Machine Learning-Assisted Identification and Detection of Thiols

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(51), P. 71048 - 71059

Published: Dec. 12, 2024

Given the crucial role of thiols in maintaining normal physiological functions, it is essential to establish a high-throughput and sensitive analytical method identify quantify various accurately. Inspired by iron porphyrin active center natural horseradish peroxidase (HRP), we designed synthesized two covalent organic frameworks (Fe-COF-H Fe-COF-OH) with notable peroxidase-like (POD) activity, capable catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB three distinct absorption peaks. Based on these, six-channel nanozyme colorimetric sensor array was constructed, which could map specific fingerprints thiols. Subsequently, machine learning techniques, including supervised linear discriminant analysis (LDA), decision trees (DT) artificial neural networks (ANN), unsupervised hierarchical cluster (HCA), ensemble random forests (RF), were used for precise identification complex systems, detection limit as low 50 nM. Significantly, demonstrated strong potential practical applications, analyzing homocysteine (Hcy) human serum, mercaptoacetic acid (TGA) depilatory creams, glutathione (GSH) cell lysates, thereby showing promise use disease diagnosis.

Language: Английский

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Dip-Coating Fabrication of All-Polymer Multilayer Photonic Crystals through 3D Printer Conversion

ACS Applied Polymer Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Dip-coating plays a crucial role in the production of polymer thin films and coatings on both laboratory industrial scales. The simplicity process, combined with its adaptability precision, makes it an invaluable technique for achieving consistent reproducible coatings, which can also be suitable photonics applications. As cheaper, more flexible alternative to commercial dip-coaters, we report conversion 3D printer designed fused deposition modeling into dip-coating system fabricating multilayered photonic crystals. feasibility this approach is demonstrated by distributed Bragg reflectors fluorescence planar microcavity. We used perfluorinated formulation poly(N-vinylcarbazole) as structural dielectric media, alongside recycled blend fluorescent polystyrene light emitter In cases, precise control parameters enables formation uniform nanostructures, leading spectral redistribution comparable that achieved standard spin-coated This paves way toward automating fabrication structures scale, potential scale up larger surface areas compared those obtained methods.

Language: Английский

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Colorimetric sensor technologies for quality detection in grains: A comprehensive review

Journal of Food Measurement & Characterization, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Language: Английский

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Bioinspired Spatial Compartment of Substrate Molecules and Catalytic Counting Entities in Hierarchical MOFs Initiated for a Dual-Mode Glycoprotein Assay

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Living cell systems possess multiple isolated compartments that can spatially confine complex substances and shield them from each other to allow for feedback reactions. In this work, a bioinspired design of metal–organic frameworks (MOFs) with well-defined multishelled matrices was fabricated as hierarchical host guest including fluorogenic molecules catalytic nanoparticles (NPs) at the separated locations development dual-mode glycoprotein assay. The multispatial-compartmental zeolitic imidazolate framework-8 (ZIF-8) constituents were synthesized via epitaxial shell-by-shell overgrowth guide integration spatial organization guests. specific property toward recognition guaranteed by both antibody–antigen covalent bonding through boronate-glycan affinity, immediate signal responses initiated textural collapse ZIF-8 integrity. addition, inner micropore enclosed space avoid surpassed contact between molecular entities, inherently. Furthermore, function matrix hosting abundant substrates large number Pt-shelled Au (AuPt) NPs, which signify its amplification means, while upon stimuli-responsive framework collapse, generators be harvested in on-demand manner. Besides, endowing Pt shells on inert plasmonic NPs not only mimic peroxidase-like behavior involved reaction generate fluorescence signals but also scattering reporters, dynamic light output signals. Collectively, our proposed method may provide new thought combining MOF dual-signal manner, reinforce accurate detection capability

Language: Английский

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Non-tandem enzymatic modules-encapsulated metal-organic framework cubes with mutual-validated operation for dual-modal competitive immunoassay of antibiotics

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 137160 - 137160

Published: Dec. 1, 2024

Language: Английский

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