A Comprehensive Study of Reactive Oxygen Species Explicit Dosimetry for Pleural Photodynamic Therapy DOI Creative Commons

Hongjing Sun,

Yi Hong Ong, Michele M. Kim

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1436 - 1436

Published: Nov. 22, 2024

Photodynamic therapy (PDT) relies on the interactions between light, photosensitizers, and tissue oxygen to produce cytotoxic reactive species (ROS), primarily singlet (1O2) through Type II photochemical reactions, along with superoxide anion radicals (O2•−), hydrogen peroxide (H2O2), hydroxyl (•OH) I mechanisms. Accurate dosimetry, accounting for all three components, is crucial predicting optimizing PDT outcomes. Conventional dosimetry tracks only light fluence rate photosensitizer concentration, neglecting role of oxygenation. Reactive explicit (ROSED) quantifies reacted concentration ([ROS]rx) by measurements (rate), concentration. Here we determine oxygenation from non-invasive diffuse correlation spectroscopy (DCS) measurement tumor blood flow using a conversion factor established preclinically. In this study, have enrolled 24 pleural patients into study. Of these patients, are able obtain data 20. Explicit fluence, Photofrin concentrations were integrated ROSED model calculate [ROS]rx across multiple sites inside cavity among different patients. Large inter- intra-patient heterogeneities in observed, despite identical 60 J/cm2 doses, mean [ROS]rx,meas 0.56 ± 0.26 mM 13 21 sites, [ROS]rx,calc1 0.48 0.23 20 76 sites. This study presented first comprehensive analysis clinical mesothelioma providing valuable future based that can potentially uniform ROS thus improve efficacy Photofrin-mediated PDT.

Language: Английский

Synthesis, Optical and Singlet Oxygen Generation Properties of Phenazinone‐Based Photosensitizer with 5‐Formyl‐2‐Thienyl Substituents DOI Creative Commons

Hiroto Tsuyama,

Keiichi Imato, Yousuke Ooyama

et al.

Asian Journal of Organic Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Abstract Phenazinone chromophore incorporating a carbonyl group into the skeleton is recently found to be new‐type halogen‐atom‐free‐photosensitizer possessing superior ability in singlet oxygen ( 1 O 2 ) generation. In this work, order gain insight substituent effect on optical properties and generation of phenazinone‐based PSs, we have designed developed derivative with two 5‐formyl‐2‐thienyl substituents which expected lead improvement light harvesting efficiencies. This work revealed that introduction phenazinone leads bathochromic shift photoabsorption band, but thermodynamically unfavorable energy gap (Δ E ST value between S state T n as well low intersystem crossing (ISC) constant k ISC ), resulting quantum yield, although possesses feasible characteristics based El‐Sayed's rule.

Language: Английский

Citations

0
A Comprehensive Study of Reactive Oxygen Species Explicit Dosimetry for Pleural Photodynamic Therapy DOI Creative Commons

Hongjing Sun,

Yi Hong Ong, Michele M. Kim

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1436 - 1436

Published: Nov. 22, 2024

Photodynamic therapy (PDT) relies on the interactions between light, photosensitizers, and tissue oxygen to produce cytotoxic reactive species (ROS), primarily singlet (1O2) through Type II photochemical reactions, along with superoxide anion radicals (O2•−), hydrogen peroxide (H2O2), hydroxyl (•OH) I mechanisms. Accurate dosimetry, accounting for all three components, is crucial predicting optimizing PDT outcomes. Conventional dosimetry tracks only light fluence rate photosensitizer concentration, neglecting role of oxygenation. Reactive explicit (ROSED) quantifies reacted concentration ([ROS]rx) by measurements (rate), concentration. Here we determine oxygenation from non-invasive diffuse correlation spectroscopy (DCS) measurement tumor blood flow using a conversion factor established preclinically. In this study, have enrolled 24 pleural patients into study. Of these patients, are able obtain data 20. Explicit fluence, Photofrin concentrations were integrated ROSED model calculate [ROS]rx across multiple sites inside cavity among different patients. Large inter- intra-patient heterogeneities in observed, despite identical 60 J/cm2 doses, mean [ROS]rx,meas 0.56 ± 0.26 mM 13 21 sites, [ROS]rx,calc1 0.48 0.23 20 76 sites. This study presented first comprehensive analysis clinical mesothelioma providing valuable future based that can potentially uniform ROS thus improve efficacy Photofrin-mediated PDT.

Language: Английский

Citations

0