Application of a near-infrared viscosity-responsive fluorescent probe for lysosomal targeting in fatty liver mice

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 155, P. 108162 - 108162

Published: Jan. 13, 2025

Language: Английский

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N7-methylguanosine modification in cancers: from mechanisms to therapeutic potential

Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 29, 2025

N7-methylguanosine (m7G) is an important RNA modification involved in epigenetic regulation that commonly observed both prokaryotic and eukaryotic organisms. Their influence on the synthesis processing of messenger RNA, ribosomal transfer allows m7G modifications to affect diverse cellular, physiological, pathological processes. are pivotal human diseases, particularly cancer progression. On one hand, modification-associated modulate tumour progression malignant biological characteristics, including sustained proliferation signalling, resistance cell death, activation invasion metastasis, reprogramming energy metabolism, genome instability, immune evasion. This suggests they may be novel therapeutic targets for treatment. other aberrant expression molecules linked clinicopathological staging, lymph node unfavourable prognoses patients with cancer, indicating their potential as biomarkers. review consolidates discovery, identification, detection methodologies, functional roles modification, analysing mechanisms by which contribute development, exploring clinical applications diagnostics therapy, thereby providing innovative strategies identification targeted

Language: Английский

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Rational design of fast-responding and highly selective fluorescent probes for detecting and imaging acetylcholinesterase homeostatic changes in Alzheimer's disease

Dyes and Pigments, Journal Year: 2025, Volume and Issue: unknown, P. 112636 - 112636

Published: Jan. 1, 2025

Language: Английский

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Elucidating Cuproptosis-Linked Redox Dynamics in Wilson Disease via a Reversible Ratiometric Fluorescence Strategy

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Redox dynamics in Wilson's disease and cuproptosis were elucidated using a reversible ratiometric fluorescent probe, WD-Redox, providing insights into oxidative stress, copper metabolism disorders, therapeutic evaluations of d -penicillamine.

Language: Английский

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Advancements in small molecule fluorescent probes for the detection of formaldehyde in environmental and food samples: A comprehensive review

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144041 - 144041

Published: March 1, 2025

Language: Английский

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Monitoring Ferroptosis with NIR Fluorescence Probe Capable of Reversible Mitochondria Nucleus Translocation

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Ferroptosis, a recently proposed form of regulated cell death, is characterized by surge in reactive oxygen species and subsequent depletion glutathione. The mitochondria nucleoli play pivotal roles the process ferroptosis. Therefore, monitoring interactions between during ferroptosis crucial for clarifying its physiological pathological processes. In this study, we designed synthesized near-infrared fluorescence probe MINU, which exhibits excellent stability against biological ions pH environments. Due to cationic structure good DNA affinity, MINU can target both nucleoli. Cell imaging demonstrates that reversibly migrate response changes mitochondrial membrane potential. By detecting localization intensity signals, effectively distinguish normal cell, apoptotic ferroptotic cell. Monitoring allows us more accurately appreciate processes

Language: Английский

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Engineered Bacterial Outer Membrane Vesicles Co-Delivering Angio-3 and Doxorubicin to Enhance Tumor Therapy

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 253, P. 114707 - 114707

Published: April 18, 2025

Language: Английский

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Low-background Near-infrared Fluorescent Probe for Real-time Monitoring of β-Glucuronidase Activity in Inflammation and Therapy

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

β-Glucuronidase (GUS) is an acidic hydrolase enzyme overexpressed in various inflammatory diseases, making it a promising biomarker for inflammation. However, current tools real-time, situ imaging of GUS activity are hindered by background interference, which reduces their effectiveness dynamic biological environments. To address this challenge, we developed Ox-GUS, GUS-specific fluorescent probe with unique molecular design featuring disrupted conjugated structure. This provided Ox-GUS near-zero optical properties, significantly enhanced signal-to-noise ratio, and highly sensitive detection ability. The demonstrated fluorescence enhancement up to 400 folds response activity, limit as low 0.0035 U/mL. We successfully employed visualize real-time mouse models rheumatoid arthritis, autoimmune hepatitis, bowel disease, effectively monitored therapeutic responses. study highlights the potential robust tool advancing research on GUS-related mechanisms early diagnosis treatment monitoring diseases.

Language: Английский

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Triple-Standard Hypochlorite Quantitative Array Enabled by Precise Stokes Shift Modulation in D-π-A Chemodosimeters

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

The rational design of the D-π-A chemodosimeter with a significant Stokes shift is great importance for enhancing visualization optical sensing signals. Here, three fluorescent chemodosimeters 2-(3-cyano-4,5,5-trimethylfuran-2(5H)-ylidene) malononitrile (TCF) as electron-withdrawing group are synthesized by precisely modulating electron-releasing strength. By decreasing ability electron release, electrophilicity recognition site increased 1.449 kcal/mol, improved to 201 nm, and mode changes from fluorescence quenching ratiometric finally on. Furthermore, display superior performance toward ClO-, including low limits detection (LOD, 37.0, 5.1, 1.0 nM), rapid response (<5 s), selectivity in presence 16 kinds interferents. Moreover, practicality further validated portable triple-standard quantitative array platform, which can quantitatively detect ClO- solutions. proposed modulation strategy provide new pathway sensitive visualized identification oxidants other hazardous chemicals.

Language: Английский

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Acrylamide Induces Antiapoptotic Autophagy and Apoptosis by Activating PERK Pathway in SH-SY5Y Cells

Toxics, Journal Year: 2025, Volume and Issue: 13(1), P. 41 - 41

Published: Jan. 7, 2025

Acrylamide (ACR) is a commonly used organic compound that exhibits evident neurotoxicity in humans. Our previous studies showed the mechanisms of ACR-caused included apoptosis, PERK-mediated endoplasmic reticulum stress, and autophagy, but relationships among them were still unclear. This paper investigated PERK pathway to demonstrate mechanism ACR further. Different doses set value toxicity. Then, inhibitor autophagy inhibitor, GSK2606414 3-methyladenine (3-MA), separately inhibit activation SH-SY5Y cells under treatment. With increase dose, apoptotic rate increased dose-dependent manner. After inhibition pathway, activated apoptosis autophagosome accumulation caused by alleviated. Under 3-MA treatment, deteriorated had no significant effect on ACR-induced activation; thus, pathway-induced an antiapoptotic role this condition. provides experimental basis for exploring potential molecular targets prevent control

Language: Английский

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