Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption

Frontiers in Nanotechnology, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 17, 2025

Nanoparticles (NPs) in contact with biological fluid adsorb biomolecules into a corona. This corona comprises proteins that strongly bind to the NP (hard corona) and loosely bound (soft dynamically exchange surrounding solution. While kinetics of hard formation is relatively well understood, thanks experiments robust simulation models, experimental characterization soft present more significant challenge. Here, we review current state art introduce novel open-source computational model simulate its dynamic behavior, for which provide documentation. We focus on case transferrin (Tf) interacting polystyrene NPs as an illustrative example, demonstrating how this captures complexities offers deeper insights structure behavior. show dominated by glassy evolution relate crowding effects. work advances our understanding corona, bridging limitations improved techniques.

Language: Английский

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Fe-caffeic acid metal–polyphenol networks as acid-sensitive and self-enhanced chemodynamic agents against Staphylococcus aureus

Materials Today Communications, Journal Year: 2025, Volume and Issue: 42, P. 111509 - 111509

Published: Jan. 1, 2025

Language: Английский

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Assessing gut barrier integrity and reproductive performance following pre-mating oral administration of solid-lipid-nanoparticles designed for drug delivery

Frontiers in Toxicology, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 7, 2025

Solid lipid nanoparticles (SLNs) have gained interest as drug delivery carriers due to their efficient cellular internalization and increased therapeutic effect of the loaded drug, with minimal side effects. Although recently several studies shown possibility administer SLNs during pregnancy vehicle mRNA placenta, data about premating exposure on outcome are scant. Considering that assumption drug-delivering nanocarriers in reproductive age may potentially affect women's health, aim present study was evaluate whether repeated oral administration female mice prior mating would influence key outcomes. For this purpose, melatonin (SLN + mlt) or unloaded were orally administered CD1 at two different dosages-low (7.5 mg/kg) high (750 -three times a week for 6 weeks. Females mated monitored from conception delivery. All assessed parameters, including time pregnancy, duration, litter size, presence any gross anomalies pups, maternal biochemical parameters not significantly affected by SLN administration. Embryonic development also evaluated no effects number implantation sites, fetus numbers, incidence fetal resorptions, measurements crown-rump length, well placental weights, observed treated mothers. The impact intestinal barrier integrity inflammation both vivo vitro using an epithelial model qRT-PCR. Results showed SLNs, but mlt, integrity. variation expression inflammatory cytokines recorded, did reflect significant histological alterations maintained. further confirmed biocompatibility showing simulated barrier. In conclusion, these suggest administering vehicle, does either health development, posing risk future pregnancy.

Language: Английский

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How Advanced are Self-Assembled Nanomaterials for Targeted Drug Delivery? A Comprehensive Review of the Literature

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 2133 - 2161

Published: Feb. 1, 2025

The development of effective drug delivery systems is a key focus in pharmaceutical research, aiming to enhance therapeutic efficacy while minimizing adverse effects. Self-assembled nanostructures present promising solution due their tunable properties, biocompatibility, and ability encapsulate deliver agents specific targets. This review examines recent advancements drug-based self-assembled for targeted applications, including drug-drug conjugates, polymeric-based architectures, biomolecules, peptides, DNA, squalene conjugates amphiphilic drugs. Various strategies fabricating these are discussed, with an emphasis on the design principles mechanisms underlying self-assembly potential tissues or cells. Furthermore, integration targeting ligands, stimuli-responsive moieties imaging into explored enhanced outcomes real-time monitoring. Challenges such as stability, scalability regulatory hurdles translating from bench bedside also addressed. Drug-based represent platform developing next-generation improved reduced side

Language: Английский

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Zeroth order model for the nanoparticle biomolecular corona

Frontiers in Nanotechnology, Journal Year: 2025, Volume and Issue: 7

Published: March 18, 2025

When nano-sized objects are introduced into biological systems they almost inevitably will be exposed to a compositionally complex fluid environment. It is general observation that nanoparticles in such environments rapidly get covered by biomolecular corona composed of proteins, lipids, and sugars adsorb the particle surface. While this phenomenon has been extensively studied from an experimental point view, some extent also theoretically computationally, we still lack solid theoretical framework. Here investigate kinetics formation, particle-to-particle variability composition, spatial vicinity different biomolecules corona. We do so under assumptions adsorption occurs randomly, irreversible, there no biomolecule-biomolecule interactions. derive analytical formulae but illustrate their outcomes using for experiments reasonable parameters. Overall, our results, derived simplifying assumptions, intended as useful reference points against which both more realistic computational studies can compared.

Language: Английский

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New Approach Methods (NAMs) for Genotoxicity Assessment of Nano- and Advanced Materials; Advantages and Challenges

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Journal Year: 2025, Volume and Issue: unknown, P. 503867 - 503867

Published: March 1, 2025

Language: Английский

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A Review of Eco-Corona Formation on Micro/Nanoplastics and Its Effects on Stability, Bioavailability, and Toxicity

Water, Journal Year: 2025, Volume and Issue: 17(8), P. 1124 - 1124

Published: April 10, 2025

Micro/nanoplastics (M/NPs) have become prevalent in aquatic environments due to their widespread applications. Likewise, ubiquitous ecological macromolecules can adsorb onto M/NPs form an “eco-corona”, which significantly alters environmental behaviors including aggregation dynamics, adsorption/desorption, and bioavailability. Therefore, it is necessary analyze the role of eco-corona assessing risks M/NPs. This review systematically summarizes formation mechanisms evaluates its regulatory effects on stability ecotoxicity Compared with other (e.g., natural organic matter extracellular polymeric substances), humic acid (HA) tightly binds through electrostatic hydrophobic interactions, affecting hetero-aggregation behavior colloidal stability. In terms bioavailability, various functional groups HA surface regulate charge hydrophobicity M/NPs, thereby bioaccumulation “Trojan horse” effect. Notably, corona alleviates M/NPs-induced growth inhibition oxidative stress. Genotoxicity assessment further showed that expression genes related stress response detoxification pathways. Future studies should focus synergistic between co-existing pollutants complex elucidate long-term associated formation.

Language: Английский

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Dispersion protocols have minimal impact on the biomolecular corona of advanced nanomaterials in cell culture assays

NanoImpact, Journal Year: 2025, Volume and Issue: unknown, P. 100560 - 100560

Published: April 1, 2025

Language: Английский

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