Enhancement of Indigo Production by a Metabolically Engineered Escherichia coli MG1655 Using Membrane Engineering and Two-Stage Cultivation DOI
Heng Hu, Zeyu Li, Roulin Chen

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Indigo, a natural blue pigment extensively used in the food and textile industries, faces sustainability challenges due to toxic chemicals its industrial synthesis. In this study, an efficient E. coli MG1655 system was developed for indigo biosynthesis. The strain engineered by genomic integration plasmid-based expression of Methylophaga aminisulfidivorans flavin-containing monooxygenase (MaFMO) endogenous tryptophanase (TnaA). To optimize metabolic flux toward production, key competitive pathway genes were deleted, membrane engineering introduced alleviate toxicity improve product secretion. A two-stage fermentation strategy with controlled feeding tryptophan substrate surfactants further optimized production. integrated approach achieved maximum titer 3.9 g/L 5 L fed-batch fermentation. This study highlights successful genetic strategies enhance microbial offering green alternative chemical synthesis potential applications food-grade colorant production industries.

Language: Английский

Pioneering Women Shaping the Field of Biocatalysis DOI
Ana I. Benítez‐Mateos, Caroline E. Paul, Sandy Schmidt

et al.

ChemCatChem, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract In this perspective article, we celebrate the accomplishments of female‐led research groups in biocatalysis. Through initiative, aim to showcase breadth and excellence women's increase their visibility within catalysis community. The authors wish emphasize that article represents only a small selection extraordinary women who have shaped field biocatalysis over time. Among them are scientists directly or significantly influenced inspired authors’ scientific journeys.

Language: Английский

Citations

0
Enhancement of Indigo Production by a Metabolically Engineered Escherichia coli MG1655 Using Membrane Engineering and Two-Stage Cultivation DOI
Heng Hu, Zeyu Li, Roulin Chen

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Indigo, a natural blue pigment extensively used in the food and textile industries, faces sustainability challenges due to toxic chemicals its industrial synthesis. In this study, an efficient E. coli MG1655 system was developed for indigo biosynthesis. The strain engineered by genomic integration plasmid-based expression of Methylophaga aminisulfidivorans flavin-containing monooxygenase (MaFMO) endogenous tryptophanase (TnaA). To optimize metabolic flux toward production, key competitive pathway genes were deleted, membrane engineering introduced alleviate toxicity improve product secretion. A two-stage fermentation strategy with controlled feeding tryptophan substrate surfactants further optimized production. integrated approach achieved maximum titer 3.9 g/L 5 L fed-batch fermentation. This study highlights successful genetic strategies enhance microbial offering green alternative chemical synthesis potential applications food-grade colorant production industries.

Language: Английский

Citations

0