Rapid and Precise Treatment Selection for Antimicrobial-resistant Infection Enabled by a Nano-dilution SlipChip DOI
Qi Wang, Xiang Li, Yongzhi Ren

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 117084 - 117084

Published: Dec. 24, 2024

Language: Английский

CRISPR/Cas12a-based transition state molecular switch for low-background detection of HPV-16 on a microfluidic platform DOI

Boren Sui,

Chunhong Liu, Zhiwei Sun

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143556 - 143556

Published: April 28, 2025

Language: Английский

Citations

0
Development of a PCR method for rapid detection of Leptospira from one microliter of whole blood DOI Creative Commons
Satoshi Miyahara, Toshiyuki Yoneda, Satoshi Kimura

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 113(1), P. 116894 - 116894

Published: May 5, 2025

Language: Английский

Citations

0
Electrostatic Microfiltration (EM) Enriches and Recovers Viable Microorganisms at Low-abundance in Large-volume Samples and Enhances Downstream Detection DOI Creative Commons
Yaoping Liu, Joshua Jebaraj Raymond, Xiaolin Wu

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(18), P. 4275 - 4287

Published: Jan. 1, 2024

Rapid and sensitive detection of pathogens in various samples is crucial for disease diagnosis, environmental surveillance, as well food water safety monitoring. However, the low abundance (<10 CFU) large volume (1 mL-1 L) containing vast backgrounds critically limits sensitivity even most advanced techniques, such digital PCR. Therefore, there a critical need sample preparation that can enrich low-abundance from complex large-volume samples. This study develops an efficient electrostatic microfiltration (EM)-based technique capable processing ultra-large-volume (≥500 mL) at high throughput (≥10 mL min

Language: Английский

Citations

3
Detection of genetically modified organisms using highly multiplexed amplicon sequencing DOI Creative Commons
C. Sarai Reyes-Avila, Dominique Waldvogel,

Nicolas Pradervand

et al.

Food Control, Journal Year: 2024, Volume and Issue: 165, P. 110670 - 110670

Published: June 22, 2024

The circulation of products based on genetically modified (GM) organisms is highly regulated by some governments with strict implementation rules for the breeding, planting, marketing, labelling, and trading such products. To ensure compliance, accurate detection methods GM events are necessary, along assurance that material falls within relevant threshold levels. increasing complexity potential undocumented a growing challenge genetic screening. Here, we developed assessed multiplexed amplicon sequencing assay microfluidics platform next-generation (NGS). probe comprehensively, designed total 230 new amplicons to cover flanking, promoter, junction, coding sequences sequences. In addition, implemented parallel amplification ribosomal chloroplast markers define crop species identity from potentially mixed samples. Using reference 11 multiple amplicons, successfully detected presence 10 known modifications per event. We also find reported flanking may not be all useful diagnostic. assay's detect in samples as well diluted DNA. Finally, performed prospective search plant material. Our microfluidics-based approach fills important gaps detecting complex recovering wide range specific evaluation. Integrating assays screening efforts should an effective complement aid post-market monitoring regulatory compliance efforts.

Language: Английский

Citations

1
Advances in virus-host interaction research based on microfluidic platforms DOI

Cheng Wang,

Ji Wang, Dong Liu

et al.

Chinese Chemical Letters, Journal Year: 2024, Volume and Issue: 35(12), P. 110302 - 110302

Published: July 31, 2024

Language: Английский

Citations

1
A novel nucleic acid extraction system integrating a switching valve-assisted microfluidic cartridge and a constant pressure pump DOI

Jiujian Zhou,

Anzhong Hu,

Jun Zhao

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1330, P. 343289 - 343289

Published: Sept. 28, 2024

Language: Английский

Citations

0
Advancing healthcare through laboratory on a chip technology: Transforming microorganism identification and diagnostics DOI
Carlos M. Ardila

World Journal of Clinical Cases, Journal Year: 2024, Volume and Issue: 13(3)

Published: Nov. 8, 2024

In a recent case report in the World Journal of Clinical Cases, emphasized crucial role rapidly and accurately identifying pathogens to optimize patient treatment outcomes. Laboratory-on-a-chip (LOC) technology has emerged as transformative tool health care, offering rapid, sensitive, specific identification microorganisms. This editorial provides comprehensive overview LOC technology, highlighting its principles, advantages, applications, challenges, future directions. Success studies from field have demonstrated practical benefits devices clinical diagnostics, epidemiology, food safety. Comparative underscored superiority over traditional methods, showcasing improvements speed, accuracy, portability. The integration with biosensors, artificial intelligence, data analytics promises further innovation expansion. call action emphasizes importance continued research, investment, adoption realize full potential improving healthcare outcomes worldwide.

Language: Английский

Citations

0
Advancements in microfluidic platforms for rapid biomarker diagnostics of infectious diseases DOI
Asma Vafadar, Sara Takallu, Shayan Khalili Alashti

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 112296 - 112296

Published: Nov. 1, 2024

Language: Английский

Citations

0
Rapid and Precise Treatment Selection for Antimicrobial-resistant Infection Enabled by a Nano-dilution SlipChip DOI
Qi Wang, Xiang Li, Yongzhi Ren

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 117084 - 117084

Published: Dec. 24, 2024

Language: Английский

Citations

0