Precision Microfluidic Control of Neuronal Ensembles in Cultured Cortical Networks DOI Creative Commons
Hakuba Murota, Hideaki Yamamoto,

Nobuaki Monma

et al.

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

Abstract In vitro neuronal culture is an important research platform in cellular and network neuroscience. However, neurons cultured on a homogeneous scaffold form dense, randomly connected networks display excessively synchronized activity; this phenomenon has limited their applications network‐level studies, such as studies of ensembles, or coordinated activity by group neurons. Herein, polydimethylsiloxane‐based microfluidic devices are developed to create small exhibiting hierarchically modular structure resembling the connectivity observed mammalian cortex. The strength intermodular coupling manipulated varying width height microchannels that connect modules. Neuronal recording via calcium imaging shows spontaneous with smaller (2.2–5.5 µm 2 ) lower synchrony exhibits threefold variety ensembles. Optogenetic stimulation demonstrates reduction enriches evoked patterns repeated induces plasticity ensembles these networks. These findings suggest cell engineering technologies based enable reconstruction intricate dynamics thus providing robust for studying well‐defined physicochemical environment.

Language: Английский

Additive manufacturing in spatial patterning for spinal cord injury treatment DOI
Christy Kwokdinata, Sing Yian Chew

Advanced Drug Delivery Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 115523 - 115523

Published: Jan. 1, 2025

Language: Английский

Citations

0
An in vitro platform for characterizing axonal electrophysiology of individual human iPSC-derived nociceptors DOI
Blandine Clément,

Lorenzo Petrella,

Lea Wallimann

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117418 - 117418

Published: April 1, 2025

Language: Английский

Citations

0
Chemical Synthesis and Chaperone Peptide Mediated Folding of Human Nerve Growth Factor by Expressed KAHA Ligation DOI Creative Commons

Nicolas Yannick Nötel,

Angus E. McMillan,

Vijaya R. Pattabiraman

et al.

ACS Central Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Engineered biological neuronal networks as basic logic operators DOI Creative Commons

Joël Küchler,

Katarina Vulić, Haotian Yao

et al.

Frontiers in Computational Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: April 28, 2025

We present an in vitro neuronal network with controlled topology capable of performing basic Boolean computations, such as NAND and OR. Neurons cultured within polydimethylsiloxane (PDMS) microstructures on high-density microelectrode arrays (HD-MEAs) enable precise interaction through extracellular voltage stimulation spiking activity recording. The architecture our system allows for creating non-linear functions two inputs one output. Additionally, we analyze various encoding schemes, comparing the limitations rate coding potential advantages spike-timing-based strategies. This work contributes to advancement hybrid intelligence biocomputing by offering insights into neural information decoding create fully biological computational systems.

Language: Английский

Citations

0
Precision Microfluidic Control of Neuronal Ensembles in Cultured Cortical Networks DOI Creative Commons
Hakuba Murota, Hideaki Yamamoto,

Nobuaki Monma

et al.

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 23, 2024

Abstract In vitro neuronal culture is an important research platform in cellular and network neuroscience. However, neurons cultured on a homogeneous scaffold form dense, randomly connected networks display excessively synchronized activity; this phenomenon has limited their applications network‐level studies, such as studies of ensembles, or coordinated activity by group neurons. Herein, polydimethylsiloxane‐based microfluidic devices are developed to create small exhibiting hierarchically modular structure resembling the connectivity observed mammalian cortex. The strength intermodular coupling manipulated varying width height microchannels that connect modules. Neuronal recording via calcium imaging shows spontaneous with smaller (2.2–5.5 µm 2 ) lower synchrony exhibits threefold variety ensembles. Optogenetic stimulation demonstrates reduction enriches evoked patterns repeated induces plasticity ensembles these networks. These findings suggest cell engineering technologies based enable reconstruction intricate dynamics thus providing robust for studying well‐defined physicochemical environment.

Language: Английский

Citations

0