Continuous and straightforward sorting of particles in microcavities with side outlets using inertial microfluidics DOI
Feng Shen, Jie Zhang, Chenchen Zhang

et al.

Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(3)

Published: March 1, 2025

Inertial microfluidic technology has emerged as a highly promising approach for the separation of particles/cells, characterized by high throughput and label-free features. This study presents novel inertial chip design that enables continuous target particles at low Reynolds numbers (Re ≤ 36). To enhance its sorting performance, influences = 3.7–60), particle sizes (d 10 20 μm), outlet flow rates on efficiency purity are further examined, phase diagram optimal working conditions is obtained. The evolution field structure within comprehensively analyzed, which can be divided into three distinct regions, namely, main flow, sheath vortex. mechanism migration behavior across curved streamlines explored. device achieve maximum 94% large with fivefold increase in enrichment concentration, 31.3-fold purity, removal small reaching 97.1%. results demonstrate this facilitate direct larger based their size, presenting numerous advantages, such short microchannel length, number, minimal cell damage, ease operation. Hence, method represents an easy-to-use straightforward techniques anticipated to have practical application rare circulating tumor cells from complex solutions.

Language: Английский

Analysis of Single Nuclei in a Microfluidic Cytometer Towards Metaphase Enrichment DOI
Cristian Brandi, Adele De Ninno,

Filippo Ruggiero

et al.

Electrophoresis, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

ABSTRACT Identifying analyzable metaphase chromosomes is crucial for karyotyping, a common procedure used by clinicians to diagnose genetic disorders and some forms of cancer. This task often laborious time‐consuming, making it essential develop automated, efficient, reliable methods assist clinical technicians. In this work, an original label‐free microfluidic approach identify potential metaphases developed that uses impedance‐based detection individual flowing nuclei machine‐learning‐based processing synchronized high‐speed videos. Specifically, impedance signals are nucleus‐containing frames, which then processed extract the contour each nucleus. Feature extraction performed, both unsupervised supervised classification approaches implemented from those features. The proposed framework tested on K562 cells, highest accuracy obtained with coupled feature selection Synthetic Minority Over‐sampling Technique (SMOTE). Overall, study encourages future developments aimed at integrating sorting functionality in device, thus achieving effective system enrichment.

Language: Английский

Citations

0
Continuous and straightforward sorting of particles in microcavities with side outlets using inertial microfluidics DOI
Feng Shen, Jie Zhang, Chenchen Zhang

et al.

Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(3)

Published: March 1, 2025

Inertial microfluidic technology has emerged as a highly promising approach for the separation of particles/cells, characterized by high throughput and label-free features. This study presents novel inertial chip design that enables continuous target particles at low Reynolds numbers (Re ≤ 36). To enhance its sorting performance, influences = 3.7–60), particle sizes (d 10 20 μm), outlet flow rates on efficiency purity are further examined, phase diagram optimal working conditions is obtained. The evolution field structure within comprehensively analyzed, which can be divided into three distinct regions, namely, main flow, sheath vortex. mechanism migration behavior across curved streamlines explored. device achieve maximum 94% large with fivefold increase in enrichment concentration, 31.3-fold purity, removal small reaching 97.1%. results demonstrate this facilitate direct larger based their size, presenting numerous advantages, such short microchannel length, number, minimal cell damage, ease operation. Hence, method represents an easy-to-use straightforward techniques anticipated to have practical application rare circulating tumor cells from complex solutions.

Language: Английский

Citations

0