Impedimetric Biosensors for the Quantification of Serum Biomarkers for Early Detection of Lung Cancer DOI Creative Commons

Marziyeh Arabnejad,

Ibtisam E. Tothill, Iva Chianella

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 624 - 624

Published: Dec. 18, 2024

Lung cancer is the most common type of diagnosed worldwide and also among fatal. Early detection, before symptoms become evident, fundamental for patients’ survival. Therefore, several lung biomarkers have been proposed to enable a prompt diagnosis, including neuron-specific enolase (NSE) carcinoembryonic antigen (CEA). NSE CEA are two serum proteins whose elevated levels associated with cancer. Hence, in this study, impedimetric biosensors (immunosensors) able quantify were developed as proof-of-concept devices diagnosis. The sensing platform exploited immunosensors comprises novel combination magnetic platform, screen-printed gold electrode (SPGE), nanobeads (MB). MB functionalized antibodies capture analyte from sample move it over area. then by immobilizing another set either or on SPGE through formation self-assembled monolayer (SAM). second enabled sandwich assay be formed surface sensor, while manipulation was applied during sensor performance depict microfluidic system increase antigen–antibody complex prior detection quantification. optimized successfully tested measure various concentrations (0–100 ng/mL) both phosphate buffer 100% human samples. Clinically relevant limits 0.26 ng/mL 0.18 0.76 0.52 NSE, respectively, achieved via electrochemical impedance spectroscopy use potassium ferri/ferrocyanide redox probe. demonstrated great potential tools implemented early

Language: Английский

Recent Advancements and Emerging Techniques in Nucleic Acid Isolation, Amplification, and Detection from Diverse Complex Matrices of Human Interest DOI
Shashini De Silva, Seong-Soo Lee, Michael J. Dugan

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118172 - 118172

Published: Jan. 1, 2025

Language: Английский

Citations

0
Impedimetric Biosensors for the Quantification of Serum Biomarkers for Early Detection of Lung Cancer DOI Creative Commons

Marziyeh Arabnejad,

Ibtisam E. Tothill, Iva Chianella

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 624 - 624

Published: Dec. 18, 2024

Lung cancer is the most common type of diagnosed worldwide and also among fatal. Early detection, before symptoms become evident, fundamental for patients’ survival. Therefore, several lung biomarkers have been proposed to enable a prompt diagnosis, including neuron-specific enolase (NSE) carcinoembryonic antigen (CEA). NSE CEA are two serum proteins whose elevated levels associated with cancer. Hence, in this study, impedimetric biosensors (immunosensors) able quantify were developed as proof-of-concept devices diagnosis. The sensing platform exploited immunosensors comprises novel combination magnetic platform, screen-printed gold electrode (SPGE), nanobeads (MB). MB functionalized antibodies capture analyte from sample move it over area. then by immobilizing another set either or on SPGE through formation self-assembled monolayer (SAM). second enabled sandwich assay be formed surface sensor, while manipulation was applied during sensor performance depict microfluidic system increase antigen–antibody complex prior detection quantification. optimized successfully tested measure various concentrations (0–100 ng/mL) both phosphate buffer 100% human samples. Clinically relevant limits 0.26 ng/mL 0.18 0.76 0.52 NSE, respectively, achieved via electrochemical impedance spectroscopy use potassium ferri/ferrocyanide redox probe. demonstrated great potential tools implemented early

Language: Английский

Citations

1