Interaction of Polystyrene Nanoplastics with Biomolecules and Environmental Pollutants: Effects on Human Hepatocytes DOI Open Access
Barbara Mognetti, Claudio Cecone,

Katia Fancello

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2899 - 2899

Published: March 22, 2025

The inevitable exposure of humans to micro/nanoplastics has become a pressing global environmental issue, with growing concerns regarding their impact on health. While the direct effects human health remain largely unknown, increasing attention is being given potential role as carriers pollutants and organic substances. This study investigates toxicity 500 nm polystyrene nanoplastics (NPs) hepatocytes (HepG2) in vitro, both alone combination cadmium (Cd), hazardous heavy metal prevalent pollutant. One-hour 100 µg/mL NPs causes significant increase ROS production (+25% compared control) but cell viability remains unaffected even at concentrations much higher than levels. Interestingly, significantly reduce Cd cytotoxicity LC50 (cell control: 55.4% for 50 µM Cd, 66.9% + 10 NPs, 68.4% NPs). Additionally, do not alter cellular lipid content after short-term (24 h). However, when fatty acids are added medium, appear sequester acids, reducing availability impairing uptake by cells dose-dependent manner. We confirmed Dynamic Light Scattering Scanning Electron Microscopy interaction between free acids. Although exhibited minimal our experimental model, collectively findings suggest that predicting extremely challenging, due specific components biological matrix.

Language: Английский

Fabrication of Organ-on-a-Chip using Microfluidics DOI

S. Ying-Jin,

Iván Yuste, Elena González‐Burgos

et al.

Bioprinting, Journal Year: 2025, Volume and Issue: unknown, P. e00394 - e00394

Published: Jan. 1, 2025

Language: Английский

Citations

0
Interaction of Polystyrene Nanoplastics with Biomolecules and Environmental Pollutants: Effects on Human Hepatocytes DOI Open Access
Barbara Mognetti, Claudio Cecone,

Katia Fancello

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2899 - 2899

Published: March 22, 2025

The inevitable exposure of humans to micro/nanoplastics has become a pressing global environmental issue, with growing concerns regarding their impact on health. While the direct effects human health remain largely unknown, increasing attention is being given potential role as carriers pollutants and organic substances. This study investigates toxicity 500 nm polystyrene nanoplastics (NPs) hepatocytes (HepG2) in vitro, both alone combination cadmium (Cd), hazardous heavy metal prevalent pollutant. One-hour 100 µg/mL NPs causes significant increase ROS production (+25% compared control) but cell viability remains unaffected even at concentrations much higher than levels. Interestingly, significantly reduce Cd cytotoxicity LC50 (cell control: 55.4% for 50 µM Cd, 66.9% + 10 NPs, 68.4% NPs). Additionally, do not alter cellular lipid content after short-term (24 h). However, when fatty acids are added medium, appear sequester acids, reducing availability impairing uptake by cells dose-dependent manner. We confirmed Dynamic Light Scattering Scanning Electron Microscopy interaction between free acids. Although exhibited minimal our experimental model, collectively findings suggest that predicting extremely challenging, due specific components biological matrix.

Language: Английский

Citations

0