Squaraine-Peptide Conjugates as Efficient Reporters of Neutrophil Extracellular Traps-Mediated Chronic Inflammation

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

The excessive and uncontrolled release of neutrophil extracellular traps (NETs) is increasingly linked to the pathogenesis various inflammatory diseases, cardiovascular disorders, cancers. Real-time, non-invasive detection NETs crucial for understanding their role in disease progression developing targeted therapies. Current methods often lack necessary specificity resolution, particularly vivo ex settings. To address this, we have developed novel near-infrared squaraine-peptide conjugates by rational molecular design as reporters NETosis targeting protease activity elastase (NE). These self-quenching, cell-impermeable probes enable precise real-time imaging NETs. Förster resonance energy transfer (FRET)-based probe, Hetero-APA, demonstrated high detecting vitro vivo, generating strong fluorescence NETs-rich environments. overcome limitations FRET-based imaging, designed SQ-215-NETP, a non-FRET-based probe that covalently binds NE. SQ-215-NETP achieved an unprecedented resolution 90 nm/pixel human coronary thrombi, marking first report such with low weight probe. Additionally, effectively detected flow cytometry. results highlight potential these detection, offering promising tools enhanced diagnostics therapeutic strategies managing NET-mediated diseases

Language: Английский

D-(+)-Biotinylated squaraine dyes: A journey from synthetic conception, photophysical and -chemical characterization, to the exploration of their photoantitumoral action mechanisms

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 293, P. 117699 - 117699

Published: April 28, 2025

Language: Английский

NIR-Sensitive Squaraine Dye—Peptide Conjugate for Trypsin Fluorogenic Detection

Biosensors, Journal Year: 2024, Volume and Issue: 14(10), P. 458 - 458

Published: Sept. 25, 2024

Trypsin enzyme has gained recognition as a potential biomarker in several tumors, such colorectal, gastric, and pancreatic cancer, highlighting its importance disease diagnosis. In response to the demand for rapid, cost-effective, real-time detection methods, we present an innovative strategy utilizing design synthesis of NIR-sensitive dye-peptide conjugate (

Language: Английский