International Symposium on Ruminant Physiology: Current perspective on rumen microbial ecology to improve fiber digestibility DOI Creative Commons
J.L. Firkins,

E. N. Henderson,

Hou Duan

et al.

Journal of Dairy Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Although cellulose has received the most attention, further research is needed for a complete comprehension of other fiber components in forage and nonforage sources corresponding with array enzymes depolymerization resulting fermentation sugars. The carbohydrate-active (CAZymes) have been described detail herein, although new information will no doubt accumulate future. Known CAZymes are attributed to taxa that easily detected via 16S rRNA gene profiling techniques, but such approaches limitations. We describe how closely related species or strains expand into different niches depending on diet dynamic availability remaining fibrous substrates. Moreover, expression as pathways can shift among even within over time incubation. unique fibrolytic bacteria, protozoa, fungi while emphasizing development consortia efficiently increase neutral detergent degradability (NDFD). For example, more powerful genome-centric functional omics combined expanded bioinformatics network analyses our current understanding ruminal function bottlenecks lead among-study variation NDFD. Specific examples highlighted include lack fundamental why starch limits NDFD, whereas moderate inclusion rumen-degraded protein, certain supplemental fatty acids (especially palmitic), sugars sometimes stimulates Current future must uncover deeper complexity rumen microbiome through combination herein be followed by validation using novel cultivation studies and, ultimately, NDFD measured vivo integration ruminant productivity traits.

Language: Английский

A Non‐Metaproteomics Researchers’ View on Metaproteomics in Microbiome Research DOI Creative Commons
Velma T. E. Aho, Laure‐Alix Clerbaux, Anne Kupczok

et al.

PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

ABSTRACT Metaproteomics, an emerging field among the omic techniques, holds great promise for unraveling function of microbiomes in host health and our environment. Metaproteomics can also be a valuable addition to multiomics studies microbiome, complementing genome‐resolved metagenomics, metatranscriptomics, metabolomics. The potential advancements from metaproteomics research touch breadth disciplines, including ecology, biochemistry, immunology, medical microbiology, cell physiology, medicine, could lead both fundamental applied discoveries. However, there are significant roadblocks widespread adoption microbiome researchers. In this Viewpoint article, we highlight pivotal role by showcasing its advantages, exploring opportunities overcome challenges, paving way broader as mainstream technique. We hope that recommendations provided article will inspire new, beneficial collaborations between proteomics experts, algorithm infrastructure developers, biochemists, biologists, microbiologists, enabling construction knowledge base have immediate direct impact on

Language: Английский

Citations

0
International Symposium on Ruminant Physiology: Current perspective on rumen microbial ecology to improve fiber digestibility DOI Creative Commons
J.L. Firkins,

E. N. Henderson,

Hou Duan

et al.

Journal of Dairy Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Although cellulose has received the most attention, further research is needed for a complete comprehension of other fiber components in forage and nonforage sources corresponding with array enzymes depolymerization resulting fermentation sugars. The carbohydrate-active (CAZymes) have been described detail herein, although new information will no doubt accumulate future. Known CAZymes are attributed to taxa that easily detected via 16S rRNA gene profiling techniques, but such approaches limitations. We describe how closely related species or strains expand into different niches depending on diet dynamic availability remaining fibrous substrates. Moreover, expression as pathways can shift among even within over time incubation. unique fibrolytic bacteria, protozoa, fungi while emphasizing development consortia efficiently increase neutral detergent degradability (NDFD). For example, more powerful genome-centric functional omics combined expanded bioinformatics network analyses our current understanding ruminal function bottlenecks lead among-study variation NDFD. Specific examples highlighted include lack fundamental why starch limits NDFD, whereas moderate inclusion rumen-degraded protein, certain supplemental fatty acids (especially palmitic), sugars sometimes stimulates Current future must uncover deeper complexity rumen microbiome through combination herein be followed by validation using novel cultivation studies and, ultimately, NDFD measured vivo integration ruminant productivity traits.

Language: Английский

Citations

0