CIGB-300 reverses chemo-resistance in MDR1-transfected lung squamous cancer cells DOI Creative Commons

Meifeng Wang,

Dongfang Tang, Xiaofang Luo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract Background Inhibition of ABC transporter protein activity is considered to be the most effective method reverse multidrug resistance (MDR). In this study, we evaluated MDR reversal potential CIGB-300, a potent CK2 kinase inhibitor. Methods ABCB1 overexpressing lung adenocarcinoma NCI-H226 cells were constructed using lentivirus, and expression gene was detected by real-time fluorescence quantitative PCR Western blotting. MTT assay used assess cytotoxicity effect CIGB-300.The CIGB-300 on determined Blotting. Cell surface subcellular localization examined Flow Cytometry Immunofluorescence Staining. Rh123 efflux accumulation measured Fluorescent Enzyme Labeler Cytometry. Results significantly increased sensitivity drug-resistant drug pump (NCI-H226-ABCB1), while it had no their parental cell lines. At same time, its mechanism action may related inhibition expression, which dose-dependent, Moreover, in addition, demonstrated that reduced NFKB CDC37 proteins. Conclusions Our study elucidated reverses ABCB1-mediated inhibiting or intracellular signaling provides therapeutic strategy improve tumor chemosensitivity.

Language: Английский

Coumarin-Based Hybrid Compounds: A New Approach to Cancer Therapy DOI
Priti Kumari, Manpreet Kaur

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142149 - 142149

Published: March 1, 2025

Language: Английский

Citations

0
CIGB-300 reverses chemo-resistance in MDR1-transfected lung squamous cancer cells DOI Creative Commons

Meifeng Wang,

Dongfang Tang, Xiaofang Luo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract Background Inhibition of ABC transporter protein activity is considered to be the most effective method reverse multidrug resistance (MDR). In this study, we evaluated MDR reversal potential CIGB-300, a potent CK2 kinase inhibitor. Methods ABCB1 overexpressing lung adenocarcinoma NCI-H226 cells were constructed using lentivirus, and expression gene was detected by real-time fluorescence quantitative PCR Western blotting. MTT assay used assess cytotoxicity effect CIGB-300.The CIGB-300 on determined Blotting. Cell surface subcellular localization examined Flow Cytometry Immunofluorescence Staining. Rh123 efflux accumulation measured Fluorescent Enzyme Labeler Cytometry. Results significantly increased sensitivity drug-resistant drug pump (NCI-H226-ABCB1), while it had no their parental cell lines. At same time, its mechanism action may related inhibition expression, which dose-dependent, Moreover, in addition, demonstrated that reduced NFKB CDC37 proteins. Conclusions Our study elucidated reverses ABCB1-mediated inhibiting or intracellular signaling provides therapeutic strategy improve tumor chemosensitivity.

Language: Английский

Citations

0