A Dual-Capture and Dual-Output 3D DNA Walker System Integrated with Ligases Enables Ultrasensitive Detection of Single-Nucleotide Polymorphisms

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

DNA walkers, as structurally and functionally programmable signal amplification tools, exhibit great potential for application in the field of biosensing. Traditional walkers often rely on enzymes operation, posing compatibility challenges, while handful existing enzyme-free demonstrate limited performance. To address this, we innovatively developed an efficient 3D walker with dual capture output capabilities. Coupled ligase chain reaction (LCR), this system facilitates highly sensitive specific detection single nucleotide polymorphisms (SNPs). Specifically, LCR precisely identifies single-base mutations, effectively transmitting biological information. The is based entropy-driven circuit cycling technology. In system, products serve driving strands walker, independently binding to track walking legs immobilized gold nanoparticles, forming a unique mechanism. Each strand carries two chains, significantly enhancing efficiency. Benefiting from novel strategy, our biosensing exhibits exceptional sensitivity mutant targets (MT), detecting MT at concentrations low 30.3 aM distinguishing heterozygous samples 0.01% mutation frequency. Furthermore, has been successfully applied genotyping abundance assessment genomes fresh soybean leaves, demonstrating its vast practical applications. summary, research pioneers capabilities, develops ultrasensitive tool. This provides strong technical support advancement genetic research.

Language: Английский

From Leaf to Lab-on-Cloth: Spatial DNA Nanorobotics and 2D Graphyne Synergy Enable Ultra-Precise Electrochemical Tracking of Sugarcane Pokkah Boeng Disease

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 283, P. 117548 - 117548

Published: May 2, 2025

Language: Английский

Investigation of the DNA Methylation-Modified 8–17 DNAzyme Functions via Sensitive Catalytic Hairpin Self-Assembly Reaction

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

8-17 DNAzyme is a well-known versatile nucleic acid tool for achieving specific cleavage function, and thus, investigation of functions can prove to be great significance. The conventional epigenetic modification on may pave new way the study catalytic properties. Herein, most abundant best characterized modifications 5-methylcytosine (5mC) N6-methyladenosine (m6A) are introduced into central core stem sequence evaluate activity. modified DNAzymes arranged recognize cleave single-stranded DNA (ssDNA) substrates that contain 5'-rAG-3' motif, producing large numbers short ssDNA leaving different amounts undegraded because their disparate excision efficiency. Meanwhile, used as triggering hairpin self-assembly (CHA) reaction. Benefiting from facile sensitive CHA reaction, methylation-induced fluctuations activity directly amplified detected. Moreover, dioxygenase ten-eleven translocation protein 2 (Tet 2) offers possibility exploring reversibility methylation-modified through demethylation process. In this study, we found both 5mC m6A in circular might lead significant inhibition effect DNAzyme. However, little variation was observed when region labeled with 5mC. Additionally, alkaline condition (pH = 9.5) enabled partial recovery DNAzyme-19-5mC (∼52.9%). More impressively, these were employed regulation miRNA-21 level nonsmall cell lung cancer (A549) cells human cervical (HeLa) cells, revealing decrease intracellular content showed positive correlation death tested cells. This would hopefully advance epigenetics research dramatically expand biosensing application DNAzymes.

Language: Английский