A Dual-Capture and Dual-Output 3D DNA Walker System Integrated with Ligases Enables Ultrasensitive Detection of Single-Nucleotide Polymorphisms

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

DNA walkers, as structurally and functionally programmable signal amplification tools, exhibit great potential for application in the field of biosensing. Traditional walkers often rely on enzymes operation, posing compatibility challenges, while handful existing enzyme-free demonstrate limited performance. To address this, we innovatively developed an efficient 3D walker with dual capture output capabilities. Coupled ligase chain reaction (LCR), this system facilitates highly sensitive specific detection single nucleotide polymorphisms (SNPs). Specifically, LCR precisely identifies single-base mutations, effectively transmitting biological information. The is based entropy-driven circuit cycling technology. In system, products serve driving strands walker, independently binding to track walking legs immobilized gold nanoparticles, forming a unique mechanism. Each strand carries two chains, significantly enhancing efficiency. Benefiting from novel strategy, our biosensing exhibits exceptional sensitivity mutant targets (MT), detecting MT at concentrations low 30.3 aM distinguishing heterozygous samples 0.01% mutation frequency. Furthermore, has been successfully applied genotyping abundance assessment genomes fresh soybean leaves, demonstrating its vast practical applications. summary, research pioneers capabilities, develops ultrasensitive tool. This provides strong technical support advancement genetic research.

Language: Английский

From Leaf to Lab-on-Cloth: Spatial DNA Nanorobotics and 2D Graphyne Synergy Enable Ultra-Precise Electrochemical Tracking of Sugarcane Pokkah Boeng Disease

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117548 - 117548

Published: May 1, 2025

Language: Английский