Anchoring Effect-Induced Conformation Remodeling in Epoxy-Functionalized Covalent Organic Frameworks for Enhanced Enzymatic Efficiency DOI
Yuchen Lu, Xiaoling Wu, Hongming Lou

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Covalent organic frameworks (COFs) with tunable structures and versatile chemical functionalities offer great promise for enzyme immobilization. This study explores the "anchoring effect" induced by covalent bonding, which significantly enhances enzymatic efficiency. Glucose oxidase (GOx) was covalently immobilized onto an epoxy-functionalized COF (oCOF-TATP) incorporating epoxy groups into framework. The immobilization approach increased loading from 26 mg/g (physical adsorption) to 40 mg/g. Secondary structure analysis revealed that binding promoted a shift α-helix β-sheet structure, more substantial effect than physical adsorption. Molecular dynamics simulations further confirmed anchoring enhanced structural rigidity exposed enzyme's active site. led 8.14% increase in accessible surface area 7.55% rise hydrogen bonding interactions. These improvements resulted significant catalytic enhancements, turnover rate (kcat) efficiency (kcat/KM) of GOx ⊂ oCOF-TATP reaching 197 388% free enzyme, respectively. In glucose oxidation assays, exhibited 115% product compared GOx, maintaining over 95% activity after six reuse cycles demonstrating superior stability under various conditions. represents novel mechanism optimizing performance operational stability.

Language: Английский

Viologen-based polymers with extended π-conjugation structure to boost zinc-iodine battery performance by constructing efficient electric double layers DOI

Linyang Qiu,

Leiqian Zhang,

Zhenxing Liang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162992 - 162992

Published: April 1, 2025

Language: Английский

Citations

0
Synthesis of a high surface area porous poly(ortho amino-phenol) resin for effective adsorption of rare earth elements Nd(III) and Er (III) ions DOI
Fatimah Algarni, Emad A. Elshehy, Muhammad Kashif

et al.

Journal of the Taiwan Institute of Chemical Engineers, Journal Year: 2025, Volume and Issue: 173, P. 106153 - 106153

Published: April 29, 2025

Language: Английский

Citations

0
Anchoring Effect-Induced Conformation Remodeling in Epoxy-Functionalized Covalent Organic Frameworks for Enhanced Enzymatic Efficiency DOI
Yuchen Lu, Xiaoling Wu, Hongming Lou

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Covalent organic frameworks (COFs) with tunable structures and versatile chemical functionalities offer great promise for enzyme immobilization. This study explores the "anchoring effect" induced by covalent bonding, which significantly enhances enzymatic efficiency. Glucose oxidase (GOx) was covalently immobilized onto an epoxy-functionalized COF (oCOF-TATP) incorporating epoxy groups into framework. The immobilization approach increased loading from 26 mg/g (physical adsorption) to 40 mg/g. Secondary structure analysis revealed that binding promoted a shift α-helix β-sheet structure, more substantial effect than physical adsorption. Molecular dynamics simulations further confirmed anchoring enhanced structural rigidity exposed enzyme's active site. led 8.14% increase in accessible surface area 7.55% rise hydrogen bonding interactions. These improvements resulted significant catalytic enhancements, turnover rate (kcat) efficiency (kcat/KM) of GOx ⊂ oCOF-TATP reaching 197 388% free enzyme, respectively. In glucose oxidation assays, exhibited 115% product compared GOx, maintaining over 95% activity after six reuse cycles demonstrating superior stability under various conditions. represents novel mechanism optimizing performance operational stability.

Language: Английский

Citations

0