A fluorescent aptasensor based on gold nanoparticles for sensitive detection of ofloxacin DOI

Wei‐Chuang Kong,

Miaomiao Wu,

Chenchen Li

et al.

Published: Dec. 5, 2024

Abstract Ofloxacin (OFL), as third generation fluoroquinolone antibiotics, is commonly used to treat bacterial infections in humans and animals. However, excessive use of OFL has led significant environmental contamination, raising concerns about food safety public health. The current detection methods for residues are often limited by operational complexity, high resource consumption, constrained application scope, highlighting an urgent need more efficient accessible alternatives. In this work, we developed a simple fast fluorescence aptasensor detecting based on FRET (Förster Resonance Energy Transfer) between the FAM fluorescent group gold nanoparticles (AuNPs). nucleic acid aptamer labeled with (FAM-Apt) binds AuNPs quench due FRET. Upon addition OFL, preferentially aptamers, leading intensity FAM-Apt increase. Therefore, strategy provided possibility detection. results showed that sensor can selectively detect linear range 0-1500 nM. limit was detected be 4.84 nM under optimized conditions. serum, urine, tap-water, river water, pork, chicken milk spiked recoveries ranged from 98.00–102.56% relative standard deviations ranging 0.41–6.99%. These demonstrate offers accurate, widely applicable potential monitoring, addressing critical needs health providing effective method residue samples.

Language: Английский

A Label‐Free Fluorescence Aptasensor for the Detection of Ciprofloxacin in Actual Samples DOI

Wei‐Chuang Kong,

Xing‐Hu Wu,

Chenchen Li

et al.

ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(7)

Published: Feb. 1, 2025

Abstract Ciprofloxacin (CIP) is a third‐generation fluoroquinolone antibiotic widely used in veterinary and clinical settings. However, overuse can result the development of quinolone‐resistant pathogens, posing risks to ecosystems human health. In this work, we developed fluorescence method for detecting CIP based on FRET (Förster Resonance Energy Transfer) between fluorescein isothiocyanate (FITC) fluorescent dye graphene oxide (GO). The aptamer was immobilized GO surface through π–π stacking interaction preventing effectively FITC from coming into contact strong fluorescence. Upon addition CIP, combined preferentially leaded release GO, which made be close leading significant quenching due FRET. intensity exhibited consistent reduction proportion concentrations with 2.99–800 nM. Under same conditions, limit detection calculated 2.99 sensor serum, urine, milk spiked recoveries ranged 99.65%–100.97% relative standard deviations ranging 0.17%–1.49%, provided tool food safety control standards.

Language: Английский

Citations

1
Cobalt Oxyhydroxide Nanosheet as Sensing Probe for Fluoroquinolones Integrating Fluorescence and Mobile Device Enabled Colorimetric Approach DOI
Sunayana Bora, Chandan Upadhyay

Published: Jan. 1, 2025

Language: Английский

Citations

0
A fluorescent aptasensor based on fluorescence resonance energy transfer for the detection of ofloxacin DOI

Wei‐Chuang Kong,

Miaomiao Wu, Chenchen Li

et al.

Chemical Papers, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский

Citations

0
Hydrophobic Fully Wearable Piezoelectric WS2 Quantum Dot-Polyaniline-Based Nanogenerator DOI
Deepak Kashyap, Ashish Kumar Chaturvedi, Asokan Pappu

et al.

ACS Applied Electronic Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Language: Английский

Citations

0
Optical Sensor Based on WS2 Quantum Dots for Lamotrigine Determination DOI Creative Commons
Lina Hristova, Rut Martínez‐Moro, Esperanza Fernández-García

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Language: Английский

Citations

0
A fluorescent aptasensor based on gold nanoparticles for sensitive detection of ofloxacin DOI

Wei‐Chuang Kong,

Miaomiao Wu,

Chenchen Li

et al.

Published: Dec. 5, 2024

Abstract Ofloxacin (OFL), as third generation fluoroquinolone antibiotics, is commonly used to treat bacterial infections in humans and animals. However, excessive use of OFL has led significant environmental contamination, raising concerns about food safety public health. The current detection methods for residues are often limited by operational complexity, high resource consumption, constrained application scope, highlighting an urgent need more efficient accessible alternatives. In this work, we developed a simple fast fluorescence aptasensor detecting based on FRET (Förster Resonance Energy Transfer) between the FAM fluorescent group gold nanoparticles (AuNPs). nucleic acid aptamer labeled with (FAM-Apt) binds AuNPs quench due FRET. Upon addition OFL, preferentially aptamers, leading intensity FAM-Apt increase. Therefore, strategy provided possibility detection. results showed that sensor can selectively detect linear range 0-1500 nM. limit was detected be 4.84 nM under optimized conditions. serum, urine, tap-water, river water, pork, chicken milk spiked recoveries ranged from 98.00–102.56% relative standard deviations ranging 0.41–6.99%. These demonstrate offers accurate, widely applicable potential monitoring, addressing critical needs health providing effective method residue samples.

Language: Английский

Citations

0