An enzymatic cleavage-triggered minimally invasive nanosensor for urine-based detection of early atherosclerosis DOI Creative Commons

Zhina Wu,

Rui Liu,

Jianai Chen

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(11)

Published: March 14, 2025

Timely detection of early atherosclerosis (AS) is crucial for improving cardiovascular outcomes, creating a growing demand diagnostic tools that are simple, sensitive, and cost-effective. Here, we introduce synthetic nanosensor AS leverages the fluorescence renal clearance properties carbon quantum dots (CQDs). This nanosensor, designed to respond proteolytic activity AS-associated dysregulated enzymes, entails CQDs as signal reporters convert fluorometric readings enabling sensitive cost-effective urine-based assay detection. Our findings demonstrated provided distinct signals in atherosclerotic versus healthy mice at stages, indicating its potential. Moreover, toxicity tests showed no notable adverse effects, supporting safety applications. minimally invasive approach could facilitate personalized therapy design continuous efficacy assessment. It expected such modular platform can be integrated with simple urine offer various diseases.

Language: Английский

An enzymatic cleavage-triggered minimally invasive nanosensor for urine-based detection of early atherosclerosis DOI Creative Commons

Zhina Wu,

Rui Liu,

Jianai Chen

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(11)

Published: March 14, 2025

Timely detection of early atherosclerosis (AS) is crucial for improving cardiovascular outcomes, creating a growing demand diagnostic tools that are simple, sensitive, and cost-effective. Here, we introduce synthetic nanosensor AS leverages the fluorescence renal clearance properties carbon quantum dots (CQDs). This nanosensor, designed to respond proteolytic activity AS-associated dysregulated enzymes, entails CQDs as signal reporters convert fluorometric readings enabling sensitive cost-effective urine-based assay detection. Our findings demonstrated provided distinct signals in atherosclerotic versus healthy mice at stages, indicating its potential. Moreover, toxicity tests showed no notable adverse effects, supporting safety applications. minimally invasive approach could facilitate personalized therapy design continuous efficacy assessment. It expected such modular platform can be integrated with simple urine offer various diseases.

Language: Английский

Citations

0