Quantitative analysis of active components in Rhodiola species based on disease module-guided network pharmacology

Arabian Journal of Chemistry, Journal Year: 2023, Volume and Issue: 17(2), P. 105570 - 105570

Published: Dec. 20, 2023

Many different species of Rhodiola are traditional botanical medicines with various effects, and their roots rhizomes the medicinal parts, which have been recorded in many ancient medical books. The 2020th edition Chinese Pharmacopoeia stipulates crenulata (Hook. f. et Thoms.) H. Ohba (R. crenulata) as only species. However, wild resources R. gradually decreasing, it is difficult to achieve artificial cultivation nowadays. It important find several alternative crenulata. In present study, an integrated strategy based on chemical profiling, disease module-guided network pharmacology, quantitative analysis (CDMQ) has developed estimate characteristics, determine active components related modules, discover potential quality markers, identify a step-by-step manner for 9 Rhodiola. Firstly, total 109 compounds were identified accordance retention times, accurate masses characteristic MS/MS fragment patterns, mainly included phenylpropanoid glycosides, gallic acid flavonoids flavanols, organic acids, others. Subsequently, pharmacology was performed targets compounds. combination limit detection UPLC-QQQ-MS/MS, 12 components. enriched modules circulatory system, mouth, respiratory skin connective tissue showed close relationship efficacy also revealed that salidroside, rhodiosin, epicatechin gallate, catechin 6'-O-galloylsalidroside changed significantly be served markers Among species, kirilowii (Regel) Maxim kirilowii) sacra (Prain ex Hamet) S. Fu sacra) could probably used Overall, this study established solid foundation provided theoretical guidance prediction clinical application, control

Language: Английский

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Incorporating Network Pharmacology and Experimental Validation to Identify Bioactive Compounds and Potential Mechanisms of Digitalis in Treating Anaplastic Thyroid Cancer

ACS Omega, Journal Year: 2024, Volume and Issue: 9(13), P. 15590 - 15602

Published: March 20, 2024

Anaplastic thyroid cancer (ATC) is one of the most lethal malignant tumors for which there no effective treatment. There are an increasing number studies on herbal medicine treating tumors, and classic botanical Digitalis its active ingredients heart failure arrhythmias have been revealed to significant antitumor efficacy against a wide range tumors. However, main components molecular mechanisms anti-ATC effects not extensively studied. Here, we screened core targets verified relationship between through network pharmacology, docking, experimental validation. These experiments showed that inhibit ATC cell activity lead death apoptotic pathway.

Language: Английский

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The application of proteomics and phosphoproteomics to reveal the molecular mechanism of salidroside in ameliorating myocardial hypoxia

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e30433 - e30433

Published: April 28, 2024

Highlights•An in-depth resource for integrated quantitative proteomics and phosphoproteomics salidroside anti-hypoxia studies.•Salidroside ameliorates myocardial hypoxia induced by cobalt chloride.•Our study systematically revealed that the had effects enhancing spindle stability, maintaining cells cycle, antagonizing cell apoptosis.AbstractSalidroside (SAL), belonging to a kind of main active ingredient Rhodiola rosea, is extensively utilized prevention altitude sickness in plateau region China. However, research on systemic changes SAL at intracellular protein level still limited, especially phosphorylation level. These limitations hinder comprehensive understanding regulatory mechanisms SAL. This aimed investigate potential molecular mechanism ameliorating acute chloride using phosphoproteomics. We successfully identified 165 differentially expressed proteins 266 phosphosites H9c2 following treatment under hypoxic conditions. Bioinformatics analysis biological experiment validation significantly antagonized CoCl2-mediated cycle arrest downregulating CCND1 expression upregulating AURKA, AURKAB, CCND3 PLK1 expression. Additionally, can stabilize cytoskeleton through Kinesin Family (KIF) members Our ability protect against CoCl2-induced multiple pathways, including relieving DNA damage, apoptosis. supplies comprehension perspective alterations levels treatment, thereby expanded our knowledge anti-hypoxic Moreover, this provides valuable further investigating

Language: Английский

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Therapeutic potential of salidroside in preserving rat cochlea organ of corti from gentamicin-induced injury through modulation of NRF2 signaling and GSK3β/NF-κB pathway

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(3), P. e0298529 - e0298529

Published: March 14, 2024

Salidroside (SAL) is a phenol glycoside compound found in plants of the Rhodiola genus which has natural antioxidant and free radical scavenging properties. SAL are able to protect against manganese-induced ototoxicity. However, molecular mechanism by reduces levels reactive oxygen species (ROS) unclear. Here, we established an vitro gentamicin (GM) ototoxicity model observe protective effect on GM-induced hair cells (HC) damage. Cochlear explants postnatal day 4 rats were obtained randomly divided into six groups: two groups (treatment with 0.2 mM or 0.4 GM for 24 h); 400 μmol/L SAL-pretreated pretreatment 3 h followed treatment (0.2 mM) h; group control (normal cultured cochlear explants). The effects HC damage, mRNA protein enzymes observed. loss occurred after treatment. Pretreatment significantly reduced OHC loss. In tissues, NRF2 HO-1 enhanced alone compared group. may regulating defense system tissues; can activate NRF2/HO-1 signaling, inhibit NF-κB activation, AKT, increase inhibitory phosphorylation GSK3β decrease GSK3 activity, all exert effects.

Language: Английский

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