The Impact of Mutant EDNRB on the Two-End Black Coat Color Phenotype in Chinese Local Pigs DOI Creative Commons
Min Huang,

Zong Qi Wen,

Tao Huang

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 478 - 478

Published: Feb. 7, 2025

Endothelin Receptor Type B (EDNRB) is expressed in a variety of cells during embryonic stage, including melanocyte precursors cells. Our previous studies found that 11 bp deletion EDNRB caused the two-end black (TEB) coat color Chinese pigs. In this study, we aimed to explore mutant on formation TEB We constructed recombinant plasmid for wild and EDN1, respectively, transfected into mouse B16 melanoma groups. Real-time fluorescent quantitative PCR (RT-qPCR) was performed detect expression genes participate melanin pathway, PLCγ, Raf, MITF. Comparing wild-type cells, three cell line expressing significantly reduced. measured content produced by granulocytes amount lower than wild. Wound-healing assay confirmed migration mobility rate were Co-immunoprecipitation further could not interact with EDN1 protein. conclusion, study revealed reduced production, which may be inhibiting The explored effect pigs color, results provided reference exploring molecular mechanism

Language: Английский

The Impact of Mutant EDNRB on the Two-End Black Coat Color Phenotype in Chinese Local Pigs DOI Creative Commons
Min Huang,

Zong Qi Wen,

Tao Huang

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 478 - 478

Published: Feb. 7, 2025

Endothelin Receptor Type B (EDNRB) is expressed in a variety of cells during embryonic stage, including melanocyte precursors cells. Our previous studies found that 11 bp deletion EDNRB caused the two-end black (TEB) coat color Chinese pigs. In this study, we aimed to explore mutant on formation TEB We constructed recombinant plasmid for wild and EDN1, respectively, transfected into mouse B16 melanoma groups. Real-time fluorescent quantitative PCR (RT-qPCR) was performed detect expression genes participate melanin pathway, PLCγ, Raf, MITF. Comparing wild-type cells, three cell line expressing significantly reduced. measured content produced by granulocytes amount lower than wild. Wound-healing assay confirmed migration mobility rate were Co-immunoprecipitation further could not interact with EDN1 protein. conclusion, study revealed reduced production, which may be inhibiting The explored effect pigs color, results provided reference exploring molecular mechanism

Language: Английский

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