Selected flavonoids exhibit antibiofilm and antibacterial effects against Vibrio by disrupting membrane integrity, virulence and metabolic activities

Biofilm, Journal Year: 2023, Volume and Issue: 6, P. 100165 - 100165

Published: Nov. 7, 2023

Vibrio parahaemolyticus is a high-risk foodborne pathogen associated with raw or undercooked seafoods and its biofilm forming potential has become threat to food safety economic values. Hence, this study aims examine the antibacterial antibiofilm activities as well virulence inhibitory effects of selected flavonoids against V. parahaemolyticus. Out sixteen flavonoid derivatives, 6-aminoflavone (6-AF), 3,2-dihydroxyflavone (3,2-DHF) 2,2-dihydroxy-4-methoxybenzophenone (DHMB) were found active inhibitors. 3,2-DHF DHMB had minimum concentrations 20 50 μg/mL respectively planktonic cells displayed superior standard controls. Also, they disrupted preformed biofilms suppressed properties including motilities, cell hydrophobicity aggregation. They impaired iron acquisition mechanism hemolysin production at sub-MICs supported by transcriptomic studies. Interestingly, interfered metabolic activity, division membrane permeability exert activities. 6-AF non-toxic in C. elegans model showed excellent capacity protect shrimps from biodeterioration. Furthermore, inhibited formation harveyi, Staphylococcus aureus Salmonella typhimurium mixed-species Vibrio. This discovered especially bioactive compounds capable offering protection risks other pathogens.

Language: Английский

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Influence of selected plant extracts on bacterial motility, aggregation, hydrophobicity, exopolysaccharide production and quorum sensing during biofilm formation of enterohaemorrhagic Escherichia coli O157:H7

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 167, P. 197 - 208

Published: Feb. 20, 2024

Diarrhoeagenic Escherichia coli is a serious threat to human and animal health, with antimicrobial drug resistance contributing the problem. Alternative mechanisms of prevention treatment E. coli-induced diarrhoea include those targeting biofilm production quorum sensing, properties that have been associated some investigated plant extracts. Plants were selected from various genera, including Vachellia, Senegalia, Morus, Leucaena, Salix, Grewia, Ziziphus, Searsia, Dichrostachys Ceratonia, based on their known activity. The antibacterial effects leaf extracts against multidrug-resistant O157:H7 using broth microdilution method, cytotoxicity normal mammalian cell lines was studied tetrazolium colorimetric assay. Subsequently, antibiofilm activity inhibition extracellular polymeric substance (EPS) by crystal violet as staining dye after 0 24 h incubation carbohydrate estimation 5 % phenol-sulfuric biosensor strain Chromobacterium violaceum ATCC 12472 used study anti-quorum sensing potential Cell motility, surface hydrophobicity (CSH), aggregation also investigated. All had weak (MIC > 0.62 mg/ml) relatively low cytotoxicity. acetone Salix babylonica Leucaena leucocephala prevented bacterial attachment (0 h) 81.21 89.36 %, respectively. Approximately 28 eradicated established biofilms more than 50 however, they unable inhibit EPS above 30 %. half-maximal concentration generally inhibited violacein (ranging 0.01 0.02 mg/ml), V. erioloba extract being most effective at quenching. Some exhibited significant difference (p<0.05) in compared untreated cells. This highlights act via different action decrease virulence enteric coli, motivating further investigation bioactive compounds leaves.

Language: Английский

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Piperine, a phytochemical prevents the biofilm city of methicillin-resistant Staphylococcus aureus: A biochemical approach to understand the underlying mechanism

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 189, P. 106601 - 106601

Published: Feb. 27, 2024

Language: Английский

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Mechanisms of microbial co-aggregation in mixed anaerobic cultures

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: July 4, 2024

Abstract Co-aggregation of anaerobic microorganisms into suspended microbial biofilms (aggregates) serves ecological and biotechnological functions. Tightly packed aggregates metabolically interdependent bacteria archaea play key roles in cycling carbon nitrogen. Additionally, applications, such as wastewater treatment, provide a complete metabolic network to convert complex organic material. Currently, experimental data explaining the mechanisms behind co-aggregation anoxic environments is scarce scattered across literature. To what extent does this process resemble aerobic environments? Does limited availability terminal electron acceptors drive mutualistic relationships, contrary commensal relationships observed oxygen-rich And do co-aggregating archaea, which depend on each other harvest bare minimum Gibbs energy from energy-poor substrates, use similar cellular those used by pathogenic that form biofilms? Here, we an overview current understanding why how mixed communities co-aggregate discuss potential future scientific advancements could improve study aggregates. Key points • Metabolic dependency promotes aggregation Flagella, pili, adhesins role formation Cyclic di-GMP/AMP signaling may trigger polysaccharides production anaerobes

Language: Английский

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Ecological mechanisms of biofilm development in the hybrid sludge-biofilm process: Implications for process start-up and optimization

Water Research, Journal Year: 2023, Volume and Issue: 245, P. 120587 - 120587

Published: Sept. 6, 2023

Language: Английский

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The role of endospore appendages in spore–spore interactions in the pathogenic Bacillus cereus group

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(9)

Published: Sept. 1, 2024

Abstract Species within the Bacillus cereus sensu lato group, known for their spore‐forming ability, are recognized significant role in food spoilage and poisoning. The spores of B. adorned with numerous pilus‐like appendages, referred to as S‐ENAs L‐ENAs. These appendages thought play vital roles self‐aggregation, adhesion, biofilm formation. Our study investigates L‐ENAs, well impact various environmental factors on spore‐to‐spore contacts interaction between vegetative cells, using both bulk single‐cell approaches. findings indicate that ENAs, especially tip fibrillae, a crucial spore but not adhesion cells. absence L‐BclA, which forms L‐ENA fibrillum, reduced aggregation mediated by highlighting interconnected We also found increased salt concentrations liquid environment significantly aggregation, suggesting charge dependency spore‐spore interactions. By shedding light these complex interactions, our offers valuable insights into dynamics. This knowledge can inform future studies behaviour settings assist developing strategies manage bacterial beneficial purposes, such controlling biofilms production equipment.

Language: Английский

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Effects of dissolved oxygen accelerated P. aeruginosa on the corrosion mechanism of X70 steel in simulated marine environments

Materials Chemistry and Physics, Journal Year: 2025, Volume and Issue: unknown, P. 130478 - 130478

Published: Jan. 1, 2025

Language: Английский

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Effects of luxS gene on biofilm formation and fermentation property in Lactobacillus plantarum R

Food Research International, Journal Year: 2025, Volume and Issue: 203, P. 115862 - 115862

Published: Feb. 1, 2025

Language: Английский

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Probiotic Potential of Three Enterococcus spp. Isolated from Raw Milk: An In Vitro Assessment

International Dairy Journal, Journal Year: 2025, Volume and Issue: unknown, P. 106196 - 106196

Published: Feb. 1, 2025

Language: Английский

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Uncovering key genes of K1 capsule Cronobacter on environmental resilience

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106224 - 106224

Published: Feb. 1, 2025

Language: Английский

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