The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 12, 2025

Abstract Loss of epithelial cell polarity and tissue disorganization are hallmarks carcinogenesis, in which Ca 2+ signaling plays a significant role. Here we demonstrate that the plasma membrane pump PMCA4 (ATP2B4) is downregulated luminal breast cancer, this associated with shorter relapse-free survival patients A B1 subtype tumors. Using MCF-7 cancer model show silencing results loss while forced increase PMCA4b expression induces polarization promotes lumen formation. We identify Arf6 as regulator endocytic recycling essential for PMCA4-mediated Silencing single pmca gene Drosophila melanogaster larval salivary gland destroys morphology suggesting conserved role PMCAs morphogenesis. Our findings point to controlling polarity, maintenance normal glandular architecture.

Language: Английский

The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 21, 2024

Abstract Loss of epithelial cell polarity and tissue disorganization are hallmarks carcinogenesis, in which Ca 2+ signaling plays a significant role. Here we demonstrate that the plasma membrane pump PMCA4 (ATP2B4) is downregulated luminal breast cancer, this associated with shorter relapse-free survival patients A B1 subtype tumors. Using MCF-7 cancer model show silencing results loss while forced increase PMCA4b expression induces polarization promotes lumen formation 2D 3D cultures. We identify Arf6 as novel regulator endocytic recycling essential for regulated formation. Silencing single pmca gene Drosophila melanogaster larval salivary gland destroys morphology suggesting conserved role PMCAs morphogenesis. Our findings point to controlling polarity, maintenance normal glandular architecture.

Language: Английский