Reviewer #1 (Public Review): A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Published: June 12, 2024

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across all DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip-γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult Drosophila.

Language: Английский

Drug tolerant persister cell plasticity in cancer: A revolutionary strategy for more effective anticancer therapies DOI Creative Commons
Jun He,

Zejing Qiu,

Jingjing Fan

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Aug. 14, 2024

Non-genetic mechanisms have recently emerged as important drivers of anticancer drug resistance. Among these, the tolerant persister (DTP) cell phenotype is attracting more and attention giving a predominant non-genetic role in cancer therapy The DTP characterized by quiescent or slow-cell-cycle reversible state subpopulation inert specialization to stimuli, which tolerates exposure some extent through interaction multiple underlying recovering growth proliferation after withdrawal, ultimately leading treatment resistance recurrence. Therefore, targeting cells anticipated provide new opportunities for patients, although our current knowledge these remains limited. In this review, we comprehensive overview formation characteristics cells, investigate potential drugs (including preclinical drugs, novel use old natural products) based on different medicine models, discuss necessity feasibility anti-DTP therapy, related application forms, future issues that will need be addressed advance emerging field towards clinical applications. Nonetheless, understanding functions may enable us develop effective improve outcomes patients.

Language: Английский

Citations

11
Tumor dormancy and relapse: understanding the molecular mechanisms of cancer recurrence DOI Creative Commons
Muhammad Tufail, Canhua Jiang, Ning Li

et al.

Military Medical Research, Journal Year: 2025, Volume and Issue: 12(1)

Published: Feb. 11, 2025

Abstract Cancer recurrence, driven by the phenomenon of tumor dormancy, presents a formidable challenge in oncology. Dormant cancer cells have ability to evade detection and treatment, leading relapse. This review emphasizes urgent need comprehend dormancy its implications for recurrence. Despite notable advancements, significant gaps remain our understanding mechanisms underlying lack reliable biomarkers predicting provides comprehensive analysis cellular, angiogenic, immunological aspects dormancy. It highlights current therapeutic strategies targeting dormant cells, particularly combination therapies immunotherapies, which hold promise preventing By elucidating these proposing innovative research methodologies, this aims deepen ultimately facilitating development more effective recurrence improving patient outcomes.

Language: Английский

Citations

1
Longevity biotechnology: bridging AI, biomarkers, geroscience and clinical applications for healthy longevity DOI Creative Commons
Yihua Lyu, Qiang Fu, Dominika Wilczok

et al.

Aging, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Aging | doi:10.18632/aging.206135. Yu-Xuan Lyu, Qiang Fu, Dominika Wilczok, Kejun Ying, Aaron King, Adam Antebi, Aleksandar Vojta, Alexandra Stolzing, Alexey Moskalev, Anastasia Georgievskaya, Andrea B. Maier, Olsen, Anja Groth, Anna Katharina Simon, Anne Brunet, Aisyah Jamil, Anton Kulaga, Asif Bhatti, Benjamin Yaden, Bente Klarlund Pedersen, Björn Schumacher, Boris Djordjevic, Brian Kennedy, Chieh Chen, Christine Yuan Huang, Christoph U. Correll, Coleen T. Murphy, Collin Y. Ewald, Danica Dario Riccardo Valenzano, Dariusz Sołdacki, David Erritzoe, Meyer, A. Sinclair, Eduardo Nunes Chini, Emma C. Teeling, Eric Morgen, Verdin, Erik Vernet, Estefano Pinilla, Evandro F. Fang, Evelyne Bischof, Evi M. Mercken, Fabian Finger, Folkert Kuipers, Frank W. Pun, Gabor Gyülveszi, Gabriele Civiletto, Garri Zmudze, Gil Blander, Harold Pincus, Joshua McClure, James L. Kirkland, Peyer, Jamie N. Justice, Jan Vijg, Jennifer R. Gruhn, Jerry McLaughlin, Joan Mannick, João Passos, Joseph Baur, Joe Betts-LaCroix, John Sedivy, Speakman, Jordan Shlain, Julia von Maltzahn, Katrin I. Andreasson, Kelsey Moody, Konstantinos Palikaras, Kristen Fortney, Laura J. Niedernhofer, Lene Juel Rasmussen, Liesbeth Veenhoff, Lisa Melton, Luigi Ferrucci, Marco Quarta, Maria Koval, Marinova, Mark Hamalainen, Maximilian Unfried, Michael S. Ringel, Milos Filipovic, Mourad Topors, Natalia Mitin, Nawal Roy, Nika Pintar, Nir Barzilai, Paolo Binetti, Parminder Singh, Paul Kohlhaas, D. Robbins, Rubin, Peter O. Fedichev, Petrina Kamya, Pura Muñoz-Canoves, Rafael de Cabo, Richard G. Faragher, Rob Konrad, Roberto Ripa, Robin Mansukhani, Sabrina Büttner, Sara Wickström, Sebastian Brunemeier, Sergey Jakimov, Shan Luo, Sharon Rosenzweig-Lipson, Shih-Yin Tsai, Stefanie Dimmeler, Thomas Rando, Tim Peterson, Tina Woods, Tony Wyss-Coray, Toren Finkel, Tzipora Strauss, Vadim Gladyshev, Valter Longo, Varun Dwaraka, Vera Gorbunova, Victoria Acosta-Rodríguez, Vincenzo Sorrentino, Vittorio Sebastiano, Wenbin Li, Yousin Suh, Alex Zhavoronkov, Morten Scheibye-Knudsen, Daniela Bakula

Language: Английский

Citations

8
Altered circadian rhythm, sleep, and rhodopsin 7 –dependent shade preference during diapause in Drosophila melanogaster DOI Creative Commons

Geoff T. Meyerhof,

Sreesankar Easwaran,

Angela E. Bontempo

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(27)

Published: June 25, 2024

To survive adverse environments, many animals enter a dormant state such as hibernation, dauer, or diapause. Various Drosophila species undergo adult reproductive diapause in response to cool temperatures and/or short day-length. While flies are less active during diapause, it is unclear how environmental conditions affect circadian rhythms and sleep. Here we show that diapause-inducing temperatures, melanogaster exhibit altered activity profiles, including severely reduced morning an advanced evening peak. Consequently, the have single peak at time similar when nondiapausing take siesta. Temperatures ≤15 °C, rather than photoperiod, primarily drive this behavior. At rapidly deep-sleep lacks sleep cycles of higher require high levels stimulation for arousal. Furthermore, 25 prefer siesta shade, preference virtually eliminated 10 °C. Resting shade driven by aversion blue light sensed Rhodopsin 7 outside eyes. Flies °C neuronal markers elevated pressure, increased expression Bruchpilot Ca 2+ R5 ellipsoid body neurons. Therefore, pressure might overcome aversion. Thus, same cause arrest, preserve germline stem cells, extend lifespan, D. prone deep dramatically altered, yet rhythmic, daily patterns.

Language: Английский

Citations

4
A study of the dormant period of Thaumetopoea pityocampa (Lepidoptera: Notodontidae) in Bulgaria DOI Creative Commons
Gergana Zaemdzhikova, Md Habibur Rahman Salman

TURKISH JOURNAL OF ZOOLOGY, Journal Year: 2025, Volume and Issue: 49(1), P. 12 - 24

Published: Jan. 2, 2025

The pine processionary moth (PPM), Thaumetopoea pityocampa (Denis et Schiffermuller), is a significant pest affecting conifer forests in the Mediterranean region. This study investigated diapause mechanisms and survival strategies of PPM summer winter feeding populations Bulgaria. research was conducted from February to April 2021 at five plantation sites known for T. activity southwest Herein, 1224 specimens were collected across different developmental stages. sampled included those exhibiting typical as well atypical feeding. Morphological assessments monitoring development individuals under controlled laboratory conditions. during dormant period, duration prepupal pupal periods, incidence prolonged analyzed. Mortality rates among stages varied significantly between populations, with showing higher percentage emerged adults lower mortality rates. dynamics cocoon spinning, pupation, adult emergence highlighted distinct phenological patterns influenced mainly by altitude habitats. Although only one year considered, notably implications population management. findings underscored adaptive significance pityocampa, reflecting complex interplay genetic predisposition environmental cues. enhances our understanding synchronization PPM's life cycle offers insights into managing its impact changing climatic

Language: Английский

Citations

0
Prepupal Diapause Reduces Adult Lifespan in the Solitary Alfalfa Leafcutter Bee DOI
Priscila Karla Ferreira Santos,

A. B. Lloyd,

Carson Stoker

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
DAF-18/PTEN protects LIN-35/Rb from CLP-1/CAPN-mediated cleavage to promote starvation resistance DOI Open Access
Jingxian Chen,

Rojin Chitrakar,

L. Ryan Baugh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

ABSTRACT Starvation resistance is a fundamental trait with profound influence on fitness and disease risk. DAF-18, the C. elegans ortholog of tumor suppressor PTEN, promotes starvation resistance. PTEN dual phosphatase, DAF-18 as lipid phosphatase by antagonizing insulin/IGF PI3K signaling, activating DAF-16/FoxO. However, if or how DAF-18/PTEN protein-phosphatase activity unknown. Using genetic, genomic, bioinformatic, biochemical approaches, we identified retinoblastoma/RB protein homolog, LIN-35/Rb, critical mediator effect We show that protects LIN-35/Rb from cleavage μ-Calpain homolog CLP-1/CAPN, together repressive DREAM complex promote conclude suppressors function in linear pathway, rest functioning transcriptional effector resulting repression germline gene expression. This work significant for revealing network survival during cellular developmental quiescence.

Language: Английский

Citations

0
A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Published: Feb. 26, 2025

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across 193 DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been diapause, 89 were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen selected candidates two Dip-𝛾 Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult .

Language: Английский

Citations

0
The role of insulin receptor InR in photoperiod-regulated reproductive diapause of Chrysoperla nipponensis DOI
Xiao Wang, Minghui Xu,

Xue Kong

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104305 - 104305

Published: March 1, 2025

Language: Английский

Citations

0
DAF-18/PTEN protects LIN-35/Rb from CLP-1/CAPN-mediated cleavage to promote starvation resistance DOI Creative Commons
Jingxian Chen,

Rojin Chitrakar,

L. Ryan Baugh

et al.

Life Science Alliance, Journal Year: 2025, Volume and Issue: 8(6), P. e202403147 - e202403147

Published: April 8, 2025

Starvation resistance is a fundamental trait with profound influence on fitness and disease risk. DAF-18, the Caenorhabditis elegans ortholog of tumor suppressor PTEN, promotes starvation resistance. PTEN dual phosphatase, DAF-18 as lipid phosphatase by antagonizing insulin/IGF PI3K signaling, activating DAF-16/FoxO. However, if or how DAF-18/PTEN protein-phosphatase activity unknown. Using genetic, genomic, bioinformatic, biochemical approaches, we identified C. retinoblastoma/RB protein homolog, LIN-35/Rb, critical mediator effect We show that protects LIN-35/Rb from cleavage μ-Calpain homolog CLP-1/CAPN, together repressive DREAM complex conclude suppressors function in linear pathway, rest functioning transcriptional effector resulting repression germline gene expression. This work significant for revealing network promote survival during cellular developmental quiescence.

Language: Английский

Citations

0