Exploring Potential Diagnostic Biomarkers for Mechanical Asphyxia in the Heart Based on Proteomics Technology DOI Open Access

Yuebing Huang,

Hai Qiu,

Qianling Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12710 - 12710

Published: Nov. 26, 2024

Mechanical asphyxia presents a challenging diagnostic issue in forensic medicine due to its often covert nature, and the signs visible during an autopsy are usually not specific. Despite some progress understanding hypoxia's effects, traditional methods' inherent limitations might overlook new biomarkers mechanical asphyxia. This study employed 4D-DIA proteomics explore protein expression profiles of cardiac samples under conditions Proteomic analysis identified 271 371 differentially expressed proteins strangulation suffocation groups, respectively, compared control group. Seventy-eight were across different groups GO KEGG showed enrichment pathways, including complement coagulation cascades, cAMP cGMP-PKG signaling inflammatory mediator regulation TRP channels, phagosomes. Through stringent selection based on interactions, ALKBH5, NAA10, CLPB as potential biomarkers. ALKBH5 increased models, while NAA10 downregulated; these biomarker changes validated both animal models human samples. highlights discovering reliable biomarkers, which can enhance specificity diagnosis practice, provide insights into pathophysiological mechanisms asphyxia, offer perspectives for diagnosing

Language: Английский

METTL14 regulates proliferation and differentiation of duck myoblasts through targeting MiR-133b DOI Creative Commons

Qicheng Jiang,

Tieshan Xu, Hailong Zhou

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0320659 - e0320659

Published: March 28, 2025

The development of duck pectoral muscle has a significant impact on meat quality, and miRNA m6A modification play key roles in this process. In the early stage, by using MeRIP-seq miRNA-seq to analyze tissue embryos at day 13 (E13), 19 (E19), 27 (E27) incubation, we found that METTL14, as core component methylation transferase complex, showed differences expression different developmental stages may have an important development. study, qRT-PCR detection revealed proliferation differentiation marker genes CDK2, CyclinD1, MYOG MYHC varied stages, with highest level E13 lowest METTL14 same stage. After constructing overexpression interference vectors for promoted embryo myoblasts inhibited differentiation, while accelerated differentiation. particular, increased miR-133b, whose precursor sequence contains sites, suggesting participate regulation affecting miR-133b. This study provides new insights into molecular mechanisms offers potential targets genetic improvement muscle.

Language: Английский

Citations

1
m6A RNA methylation dynamics during in vitro maturation of cumulus-oocyte complexes derived from adult or prepubertal sheep DOI Creative Commons

Chiara Cosseddu,

Sara Succu,

Adele Frau

et al.

Journal of Assisted Reproduction and Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Language: Английский

Citations

0
METTL3-m6A methylase regulates the osteo-/odontogenic potential of stem cells from apical papilla via NFIC in apical periodontitis. DOI Creative Commons
Youyu Zhu, Xiang Ge, Zhi Chen

et al.

Experimental Cell Research, Journal Year: 2025, Volume and Issue: 448(2), P. 114576 - 114576

Published: April 23, 2025

Stem cells from the apical papilla (SCAPs) show strong odontogenic ability and can form root dentin. However, underlying mechanisms that control differentiation of SCAPs in an inflammatory environment need further exploration. In present study, we explored regulatory role METTL3 originating tooth with periodontitis. derived healthy teeth periodontitis (AP-SCAPs) were successfully isolated cultured. The expressions tumor necrosis factor-a (TNF-a) interleukin-6 (IL-6) higher AP-SCAPs. A decrease expression accompanied decreased osteo-/odontogenic Exploring on AP-SCAPs revealed overexpression upregulated silencing exerted opposite effect. Overexpression suppressed TNF-α IL-6 AP-SCAPs, whereas knockdown these enhanced expression. regulates modulates their response. Furthermore, methylation level, mRNA, protein nuclear factor-IC (NFIC) during mineralization induction. NFIC attenuated METTL3-overexpressed conclusion, METTL3-mediated-m6A via NFIC. This paper elucidates a novel mechanism regulating may be new target for regenerative endodontic treatment.

Language: Английский

Citations

0
METTL14 Promotes Vascular Smooth Muscle Cell Proliferation and Neointima Formation via m6A Methylation TEAD1 mRNA DOI
LIANG WANG, G.L Xia, Yan Tang

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(5)

Published: May 1, 2025

ABSTRACT Vascular smooth muscle cell (VSMC) proliferation and neointimal hyperplasia critically contribute to atherosclerosis post‐angioplasty restenosis. Building on our prior discovery that TEA domain transcription factor 1 (TEAD1) regulates VSMCs differentiation, we now investigate methyltransferase‐like 14 (METTL14) in vascular remodeling. METTL14 expression was significantly upregulated human carotid atherosclerotic plaques versus control arteries, correlating with dedifferentiation. This pattern recapitulated murine wire‐induced injury models during neointima formation. Functionally, overexpression suppressed contractile markers while accelerating migration coronary artery cells (HCASMCs). Conversely, knockdown attenuated injury‐induced In Vivo. Mechanistically, stabilizes TEAD1 mRNA through m6A modification at nucleotide 513, enhancing YAP1/TEAD1 signaling. Both 513nt mutation inhibitor VT103 abolished METTL14‐driven phenotypic changes, restoring differentiation suppressing proliferation. Collectively, findings establish METTL14‐mediated of as a novel mechanism promoting pathology, highlighting its therapeutic potential for cardiovascular diseases.

Language: Английский

Citations

0
METTL3 shapes m6A epitranscriptomic landscape for successful human placentation DOI
Ram Kumar,

Rajnish Kumar,

Avishek Ganguly

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 13, 2024

Methyltransferase-like 3 (METTL3), the catalytic enzyme of methyltransferase complex for m6A methylation RNA, is essential mammalian development. However, importance METTL3 in human placentation remains largely unexplored. Here, we show that a fine balance function trophoblast cells successful placentation. Both loss-of and gain-in functions are associated with adverse pregnancies. A subset recurrent pregnancy losses preterm pregnancies often loss expression progenitors. In contrast, induced fetal growth restriction (FGR). Our analyses showed maintenance TSC self-renewal their differentiation to extravillous (EVTs). TSCs promotes syncytiotrophoblast (STB) Global RNA modification METTL3-RNA interaction regulates modifications on mRNA molecules critical regulators, including

Language: Английский

Citations

2
METTL14 depletion induces trophoblast cell dysfunction by inhibiting miR–21-5p processing in an m6A-dependent manner DOI
Xiaoli Qin,

Xiaona Huo,

Junpeng Dong

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 142, P. 113200 - 113200

Published: Sept. 27, 2024

Language: Английский

Citations

1
Evidence for the potential role of m6A modification in regulating autophagy in models of amyotrophic lateral sclerosis DOI Open Access
Di An, Jingzhe Han, Pingping Fang

et al.

CytoJournal, Journal Year: 2024, Volume and Issue: 21, P. 33 - 33

Published: Sept. 30, 2024

Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease. Research indicates that N6-methyladenosine (m6A) modification plays crucial role in cellular autophagy during ALS development. This study investigates the of ALS, with focus on effect messenger ribonucleic acid m6A methylation disease progression.

Language: Английский

Citations

1
Exploring Potential Diagnostic Biomarkers for Mechanical Asphyxia in the Heart Based on Proteomics Technology DOI Open Access

Yuebing Huang,

Hai Qiu,

Qianling Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12710 - 12710

Published: Nov. 26, 2024

Mechanical asphyxia presents a challenging diagnostic issue in forensic medicine due to its often covert nature, and the signs visible during an autopsy are usually not specific. Despite some progress understanding hypoxia's effects, traditional methods' inherent limitations might overlook new biomarkers mechanical asphyxia. This study employed 4D-DIA proteomics explore protein expression profiles of cardiac samples under conditions Proteomic analysis identified 271 371 differentially expressed proteins strangulation suffocation groups, respectively, compared control group. Seventy-eight were across different groups GO KEGG showed enrichment pathways, including complement coagulation cascades, cAMP cGMP-PKG signaling inflammatory mediator regulation TRP channels, phagosomes. Through stringent selection based on interactions, ALKBH5, NAA10, CLPB as potential biomarkers. ALKBH5 increased models, while NAA10 downregulated; these biomarker changes validated both animal models human samples. highlights discovering reliable biomarkers, which can enhance specificity diagnosis practice, provide insights into pathophysiological mechanisms asphyxia, offer perspectives for diagnosing

Language: Английский

Citations

0