Structural and kinetic insights into tRNA promoter engagement by yeast general transcription factor TFIIIC DOI Creative Commons
Wolfram Seifert-Dávila, Anastasiia Chaban, Florence Baudin

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Transcription of transfer RNA (tRNA) genes by polymerase (Pol) III requires the general transcription factor IIIC (TFIIIC), which recognizes intragenic A-box and B-box DNA motifs type II gene promoters. However, underlying mechanism has remained elusive, in part due to missing structural information for recognition. In this study, we use single-particle cryogenic electron microscopy (cryo-EM) single-molecule fluorescence resonance energy (smFRET) reveal real-time kinetic insights into how 520-kDa yeast TFIIIC complex engages context a tRNA promoter. Cryo-EM structures τA τB subcomplexes bound were obtained at 3.7 2.5 Å resolution, respectively, while cryo-EM mapping determined specific distance relative orientation revealing fully engaged state TFIIIC. smFRET experiments show that overall recruitment residence times on are primarily governed recognition, footprinting suggest key role TFIIIB Pol following recognition

Language: Английский

Structural Features of DNA in tRNA Genes and Their Upstream Sequences DOI Open Access
Ekaterina A. Savina,

Tatiana G. Shumilina,

Viktoria A. Porolo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11758 - 11758

Published: Nov. 1, 2024

RNA polymerase III (Pol III) transcribes tRNA genes using type II promoters. The internal control regions contain a Box A and B, which are recognized by TFIIIC. 5'-flanking of clearly play role in the regulation transcription, but consensus sequences it have been found only some plants

Language: Английский

Citations

0
Structural and kinetic insights into tRNA promoter engagement by yeast general transcription factor TFIIIC DOI Creative Commons
Wolfram Seifert-Dávila, Anastasiia Chaban, Florence Baudin

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Transcription of transfer RNA (tRNA) genes by polymerase (Pol) III requires the general transcription factor IIIC (TFIIIC), which recognizes intragenic A-box and B-box DNA motifs type II gene promoters. However, underlying mechanism has remained elusive, in part due to missing structural information for recognition. In this study, we use single-particle cryogenic electron microscopy (cryo-EM) single-molecule fluorescence resonance energy (smFRET) reveal real-time kinetic insights into how 520-kDa yeast TFIIIC complex engages context a tRNA promoter. Cryo-EM structures τA τB subcomplexes bound were obtained at 3.7 2.5 Å resolution, respectively, while cryo-EM mapping determined specific distance relative orientation revealing fully engaged state TFIIIC. smFRET experiments show that overall recruitment residence times on are primarily governed recognition, footprinting suggest key role TFIIIB Pol following recognition

Language: Английский

Citations

0