The intraflagellar transport cycle

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Language: Английский

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G Protein‐Coupled Receptor Signal Intersection at the Primary Cilium

BioEssays, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

ABSTRACT Primary cilia are singular projections that extend from the surface of most vertebrate cell types. The area primary cilium (PC) is estimated to represent only 1/100 th total membrane an average cell. Despite this, PC provides essential contributions inter‐ and intracellular communication by housing receptors downstream effectors for myriad cell‐signaling cascades. G protein‐coupled (GPCRs) commonly enrich along ciliary membranes control a diverse range cellular behaviors signaling through shared pool effectors. This suggests hypothesis environment conducive complementary or competitive GPCR Signal Crosstalk. In this Hypothesis Bio Essay, we use Sonic Hedgehog (SHH) pathway as case study inform models how signals could intersect in suggest general strategies test each model.

Language: Английский

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Permanent cilia loss during cerebellar granule cell neurogenesis involves withdrawal of cilia maintenance and centriole capping

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(52)

Published: Dec. 20, 2024

Brain neurons utilize the primary cilium as a privileged compartment to detect and respond extracellular ligands such Sonic hedgehog (SHH). However, cilia in cerebellar granule cell (GC) disassemble during differentiation through ultrastructurally unique intermediates, process we refer deconstruction. In addition, mature do not reciliate despite having docked centrioles. Here, identify molecular changes that accompany deconstruction centriole docking GC neurons. We used single transcriptomic immunocytological analyses compare transcript levels subcellular localization of proteins between progenitor, differentiating, GCs. Differentiating GCs lacked transcripts for key activators premitotic resorption, indicating disassembly differentiating cells is distinct from resorption. Instead, differentiation, many genes required maintenance—specifically those encoding components intraflagellar transport, pericentrosomal material, centriolar satellites—decreased. The abundance several corresponding around centrosomes also decreased. These coincided with downregulation SHH signaling prior even mutant excessive activation. Finally, mother centrioles maturing recruited cap complex protein, CEP97. data suggest global, developmentally programmed decrease maintenance mediates deconstruction, while capping prevents regrowth dysregulated signaling. Our study provides mechanistic insights expanding our understanding permanent loss multiple tissue-specific contexts.

Language: Английский

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The impact of ciliary length on the mechanical response of osteocytes to fluid shear stress

Nitric Oxide, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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