Transplantation of engineered endothelial progenitor cells with H19 overexpression promotes arterial reendothelialization and inhibits neointimal hyperplasia

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 1, 2025

Endothelial injury is a key factor initiating in-stent restenosis (ISR) following peripheral artery stent implantation. Genetically modified endothelial progenitor cells (EPCs) can promote reendothelialization of injured arteries and inhibit neointimal hyperplasia. However, the role engineered EPCs overexpressing lncRNA H19 in these processes remains unclear. We constructed investigated their effects mechanisms promoting inhibiting hyperplasia both vitro vivo. Compared to normal control group, ISR patients exhibited significant reduction circulating EPCs. Engineered promoted inhibited arteries. Exogenous overexpression significantly upregulated repair-related gene S1PR3 EPCs, while opposite was also observed. Additionally, enhanced proliferation, migration, tube formation vitro; were lost with inhibition. Binding sites for H3K27 acetylation identified on promoter. Mechanistically, we found that directly interacted HDAC2, known H3K27ac deacetylase, disrupting its binding acetylation. Our findings suggest positively regulates expression by HDAC2 / binding, thereby

Language: Английский

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MSC Exosomes and MSC Exosomes Loaded with LncRNA H19 as Nanotherapeutics Regulate the Neurogenetic Potential of Müller Glial Cells in Dry Age-Related Macular Degeneration

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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LncRNA H19 promotes adipogenic differentiation disorder by sponging miR-130b-3p to upregulate PPARγ in steroid-induced osteonecrosis of the femoral head

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: April 7, 2025

Steroid-induced osteonecrosis of the femoral head (SONFH) is a debilitating condition linked to glucocorticoid-induced adipogenic dysregulation bone marrow mesenchymal stem cells (BMSCs). While long noncoding RNA H19 has been implicated in differentiation disorders across pathologies, its role SONFH remains undefined. This study investigated H19's regulatory mechanism progression. We observed significant upregulation both lesions and BMSCs from patients compared controls. Knockdown SONFH-derived suppressed peroxisome proliferator-activated receptor γ (PPARγ) expression, attenuated differentiation, reduced lipid accumulation, as evidenced by decreased Oil Red O staining FABP4 levels. Mechanistically, acted competitive endogenous (ceRNA) sponging miR-130b-3p, thereby alleviating miR-130b-3p-mediated repression PPARγ. Luciferase assays confirmed direct binding between miR-130b-3p H19/PPARγ, while rescue experiments demonstrated that inhibition reversed PPARγ downregulation induced silencing. Our findings reveal novel H19/miR-130b-3p/PPARγ axis driving SONFH, positioning potential therapeutic target. provides critical insights into epigenetic regulation BMSC lineage commitment pathogenesis, offering new avenues for intervention.

Language: Английский

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Circulating Non-Coding RNAs as Indicators of Fibrosis and Heart Failure Severity

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 553 - 553

Published: April 7, 2025

Heart failure (HF) is a leading cause of morbidity and mortality worldwide, representing complex clinical syndrome in which the heart’s ability to pump blood efficiently impaired. HF can be subclassified into heart with reduced ejection fraction (HFrEF) preserved (HFpEF), each distinct pathophysiological mechanisms varying levels severity. The progression significantly driven by cardiac fibrosis, pathological process extracellular matrix undergoes abnormal uncontrolled remodelling. Cardiac fibrosis characterized excessive protein deposition activation myofibroblasts, increasing stiffness heart, thus disrupting its normal structure function promoting lethal arrythmia. MicroRNAs, long non-coding RNAs, circular collectively known as RNAs (ncRNAs), have recently gained significant attention due growing body evidence suggesting their involvement remodelling such fibrosis. ncRNAs found peripheral blood, indicating potential biomarkers for assessing In this review, we critically examine recent advancements findings related use discuss implication development.

Language: Английский

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Linc00963 up-regulation alleviates postmenopausal osteoporosis through suppression of miR-506-3p

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 11, 2025

This study aimed to investigate the regulatory effect of linc00963 on postmenopausal osteoporosis and potential molecular mechanisms. Taking MC3T3-E1 cells as object, a cell cycle assay was used evaluate proliferation. mRNA levels Runx2, OCN, collagenia-1, OPG, RANKL RANK were detected. Dual luciferase reporter verified targeting relationship between miR-506-3p. A rat model established after ovariectomy in 32 Sprague-Dawley rats. The rats divided into sham group, OVX overexpression blank plasmid group. bone mineral density (BMD) femur measured by X-ray densitometer. Serum expression detected RT-qPCR. protein ALP, BGP serum ELISA. Cell studies have shown that alleviates down-regulating Animal showed compared with level, BMD, Ca, P, LEP, SOD, OPG group significantly decreased, while body weight, BGP, IL-6, IL-13, RANKL, increased. Compared use can improve above indexes play corresponding therapeutic menopausal Linc00963 is involved pathogenesis up-regulating miR-506-3p activating OPG/RANKL/RANK pathway. expected be target for osteoporosis.

Language: Английский

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LncRNA RMG controls liquid-liquid phase separation of MEIS2 to regulate myogenesis

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143309 - 143309

Published: April 17, 2025

Long non-coding RNAs (lncRNAs) regulate liquid-liquid phase separation (LLPS), driving the formation of biomolecular condensates essential for cellular function. However, this regulatory mechanism is yet to be reported in skeletal muscles. In study, we comprehensively analyzed lncRNAs muscle across multiple pig breeds, developmental stages, and tissues. Our analysis identified over 10,000 novel lncRNAs. We found that lnc-regulator growth (lnc-RMG) regulates myogenesis by modulating LLPS Meis homeobox 2 (MEIS2). Lnc-RMG was specifically expressed muscle, with significantly higher expression fetal stage than embryonic stage. Notably, lnc-RMG highly conserved between pigs humans exhibits similar biological functions myogenesis. Furthermore, knockdown promoted regeneration. Mechanistically, produces mature microRNA (miR)-133a-3p, which targets inhibits MEIS2 expression, thereby inhibiting LLPS. This inhibition transcription transforming factor-β receptor II (TGFβR2), ultimately regulating Overall, our findings revealed a lnc-RMG/miR-133a-3p/MEIS2/TGFβR2 axis regulated through provided new insights into molecular mechanisms drive development These highlight potential therapeutic muscle-related diseases strategies livestock improvement.

Language: Английский

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Short-Term and Long-Term Effects of Inhaled Ultrafine Particles on Blood Markers of Cardiovascular Diseases: A Systematic Review and Meta-Analysis

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(8), P. 2846 - 2846

Published: April 21, 2025

Background/Objectives: Air pollution is the highest environmental risk factor of mortality and morbidity worldwide, leading to over 4 million deaths each year. Among different air pollutants, ultrafine particles (UFPs) constitute cardiovascular diseases (CVDs). Epidemiological studies have associated UFPs with short- long-term imbalance numerous blood markers. Our objective was systematically review short-term impact UFP exposure on markers CVDs. Methods: We prepared systematic CVD meta-analyses effects high-sensitivity C-reactive protein (hsCRP) concentration. The eligibility criteria were established use Provider, Enrollment, Chain, Ownership System (PECOS) model, literature search conducted in Web Science, PubMed, Scopus databases from 1 January 2013 9 2025. bias (RoB) according a World Health Organization (WHO) template. Results: results showed an increase hsCRP as result both UFPs. Moreover, IL-6 IL-1β together other inflammatory increased after exposure. In addition, nucleic acids, among which miR-24-3p let-7d-5p, differentially expressed (DE) Chronic could lead persistent Conclusions: findings underline that may development and/or worsening outcomes fragile populations living air-polluted areas.

Language: Английский

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Transcriptomics analysis of long non-coding RNAs in smooth muscle cells from patients with peripheral artery disease and diabetes mellitus

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 14, 2024

Abstract Diabetes mellitus (DM) is a significant risk factor for peripheral arterial disease (PAD), and PAD an independent predictor of cardiovascular disorders (CVDs). Growing evidence suggests that long non-coding RNAs (lncRNAs) significantly contribute to development underlying complications, particularly affecting smooth muscle cells (SMCs). So far, no study has focused on transcriptome analysis lncRNAs in patients with without DM. Tissue samples were obtained from our Vascular Biobank. Due the sample’s heterogeneity, expression whole tissue detected only ACTA2-AS1 4.9-fold increase In contrast, transcriptomics SMCs revealed 28 differentially expressed between DM (FDR < 0.1). Sixteen unknown function, six described cancer, one connected macrophages polarisation, four associated CVDs, mainly SMC function phenotypic switch (NEAT1, MIR100HG, HIF1A-AS3, MRI29B2CHG). The enrichment additional H19, CARMN, FTX, MEG3 linked Our several diabetic physiological SMCs. These might serve as potential therapeutic targets improve within diseased and, thus, clinical outcome.

Language: Английский

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Circulating myo-miRs in physical exercise

Advanced Exercise and Health Science, Journal Year: 2024, Volume and Issue: 1(2), P. 86 - 98

Published: May 31, 2024

MicroRNAs (miRs) are pivotal in regulating gene expression and orchestrating adaptive responses to physical exercise. This review delves into the dynamic interplay between exercise circulating muscle-specific miRs (myo-miRs), with a specific focus on endurance resistance training modalities. Endurance exercise, involving moderate high-intensity activities, triggers acute sustained changes myo-miRs levels, mirroring physiological adaptations muscle tissue. Conversely, emphasizing high-intensity, short-duration elicits diverse myo-miRs, particularly older individuals. Moreover, interventions exercises yield distinct effects miR profiles, underscoring relationship type, intensity, duration. The potential of as biomarkers monitor exercise-induced mechanisms behind their release circulation promising areas for future research. Understanding role mediating systemic could pave way innovative therapeutic strategies, enhancing function promoting overall health well-being.

Language: Английский

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lncRNA HOTAIR and Cardiovascular diseases

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(5)

Published: Sept. 19, 2024

Language: Английский

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