Nootkatone mitigates periodontal inflammation and reduces alveolar bone loss via Nrf2/HO-1 and NF-κB pathways in rat model of periodontitis DOI Creative Commons

Ye Yin,

Zeyu Ma,

Peiliang Shi

et al.

Folia Histochemica et Cytobiologica, Journal Year: 2024, Volume and Issue: 62(3), P. 145 - 153

Published: Aug. 28, 2024

Introduction. Periodontitis (PD) is a chronic inflammatory disease leading to alveolar bone loss. This study investigates the effect of nootkatone and regulatory mechanism in reducing periodontal inflammation loss rat model. Material methods. Twenty male Sprague-Dawley rats were divided into control, periodontitis, nootkatone-treated groups (45 or 90 mg/kg). Ligature induction method was adopted establish PD After 21 days, received daily gavage either saline for 10 days. Alveolar assessed using micro-CT. Histological analyses included hematoxylin eosin (H&E), tartrate-resistant acid phosphatase (TRAP), Masson's trichrome stainings. Immunohistochemistry heme oxygenase 1 (HO-1) nuclear factor erythroid-2 related 2 (Nrf2) performed tissues. Content cytokines IL-1β, IL-6, TNF-α gingival tissues around ligature ELISA kits. Malondialdehyde (MDA) level superoxide dismutase (SOD) activity analyzed Western blot NF-κB expression performed. Results. Nootkatone significantly reduced distance from cementoenamel junction crest (CEJ-ABC), enhanced mineral density (BMD), volume (BV), BV/total (TV) ratio ligature-induced rats. Higher dose (90 mg/kg) did not show more significant therapeutic than lower staining showed decreased osteoclasts' number improved architecture group. cell infiltration by nootkatone-treatment increased Nrf2 HO-1 protein level, suppressing MDA levels enhancing SOD activity. Conclusions. In model, effectively mitigates through Nrf2/HO-1 pathways. These findings suggest as promising agent treatment periodontitis.

Language: Английский

Expression of MMP-14 and CD147 in Gingival Tissue of Patients With and Without Diabetes Mellitus Type II DOI Creative Commons

Ionuț Cătălin Botezatu,

Maria-Alexandra Mârțu,

Laura Stoica

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(5), P. 609 - 609

Published: March 3, 2025

Background: Diabetes mellitus (DM) is a major risk factor for the development of periodontal disease and aggravates severity conditions. Matrix metalloproteinases (MMPs) are known to degrade ligament attachment bone matrix proteins. Increased expression CD147 associated with increased synthesis several MMPs, being modulator MMP expression, including that MMP-14. The purpose this study was quantify compare expressions MMP-14 in gingival tissues patients without type 2 diabetes mellitus. Material Methods: In histological study, we included 33 subjects disease: 16 DM (test group) 17 systemically healthy (control group). Tissue fragments were processed using an immunohistochemistry technique determine immunoreactivity (IR) intensity CD147. Results: group periodontitis, 56.2% showed weak positive (+), while 43.8% had intensely (+++) Statistically significant differences between test control groups (p = 0.004, p 0.883, 0.002) found membranous IR moderate (++) CD 147, (+++). 0.001, 0.002, 0.003) Conclusions: significantly higher 147 demonstrates role these biomarkers pathology patients. It can be assumed could further investigated as potential predictive biomarkers.

Language: Английский

Citations

0
Gallic acid promotes M2 macrophage polarization through mitochondrial oxidative phosphorylation in periodontitis DOI Creative Commons
Ruobing Zhang, Wenjing Yang, Kai Li

et al.

Archives of Oral Biology, Journal Year: 2025, Volume and Issue: unknown, P. 106237 - 106237

Published: March 1, 2025

Language: Английский

Citations

0
Kaempferol combats the osteogenic differentiation damage of periodontal ligament stem cells in periodontitis via regulating EphrinB2-mediated PI3K/Akt and P38 pathways DOI
Jiao Cao, Yue Li, Mengying Si

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156733 - 156733

Published: April 1, 2025

Language: Английский

Citations

0
Nootkatone mitigates periodontal inflammation and reduces alveolar bone loss via Nrf2/HO-1 and NF-κB pathways in rat model of periodontitis DOI Creative Commons

Ye Yin,

Zeyu Ma,

Peiliang Shi

et al.

Folia Histochemica et Cytobiologica, Journal Year: 2024, Volume and Issue: 62(3), P. 145 - 153

Published: Aug. 28, 2024

Introduction. Periodontitis (PD) is a chronic inflammatory disease leading to alveolar bone loss. This study investigates the effect of nootkatone and regulatory mechanism in reducing periodontal inflammation loss rat model. Material methods. Twenty male Sprague-Dawley rats were divided into control, periodontitis, nootkatone-treated groups (45 or 90 mg/kg). Ligature induction method was adopted establish PD After 21 days, received daily gavage either saline for 10 days. Alveolar assessed using micro-CT. Histological analyses included hematoxylin eosin (H&E), tartrate-resistant acid phosphatase (TRAP), Masson's trichrome stainings. Immunohistochemistry heme oxygenase 1 (HO-1) nuclear factor erythroid-2 related 2 (Nrf2) performed tissues. Content cytokines IL-1β, IL-6, TNF-α gingival tissues around ligature ELISA kits. Malondialdehyde (MDA) level superoxide dismutase (SOD) activity analyzed Western blot NF-κB expression performed. Results. Nootkatone significantly reduced distance from cementoenamel junction crest (CEJ-ABC), enhanced mineral density (BMD), volume (BV), BV/total (TV) ratio ligature-induced rats. Higher dose (90 mg/kg) did not show more significant therapeutic than lower staining showed decreased osteoclasts' number improved architecture group. cell infiltration by nootkatone-treatment increased Nrf2 HO-1 protein level, suppressing MDA levels enhancing SOD activity. Conclusions. In model, effectively mitigates through Nrf2/HO-1 pathways. These findings suggest as promising agent treatment periodontitis.

Language: Английский

Citations

1