Cytocompatible Hyperbranched Polyesters Capable of Altering the Ca²⁺ Signaling in Neuronal Cells In Vitro

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(10), P. 6682 - 6695

Published: Oct. 10, 2024

Synthetic hyperbranched polyesters with potential therapeutic properties were synthesized using the bifunctional polyethylene glycol or PEG different molecular weights, ca., 4000, 6000, and 20,000 g/mol, trifunctional trans-aconitic acid TAA. During polycondensation, a fixed amount of was allowed to react varying amounts TAA (1:1 1:3) control branching extents. It found that synthetic had considerable yield highly water soluble. Spectroscopic data (Fourier transform infrared 1H NMR) confirmed polyester formation; percentages determined from NMR spectroscopy which varied 73% 22% among samples. As weight increased, percentage drastically dropped. All be negatively charged due ionization unreacted –COOH in branched ends at working pH (7.4). Both hydrodynamic size intrinsic viscosity reduce as extent increased. Among sets polyesters, one highest (73%) showed core–shell morphology (evident field emission scanning electron microscopy transmission studies). also exhibited efficiency toward Ca2+ influx neuronal cells unique surface. Nevertheless, this particular grade along all other grades cytocompatible induced reactive oxygen species generation. Since maximally efficient altering signaling through stronger influx, it may well tested for treating disorders vivo future.

Language: Английский

---

Membrane Associated RNA‐Containing Vesicles Regulate Cortical Astrocytic Microdomain Calcium Transients in Awake Ischemic Stroke Mice

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Astrocytic processes minutely regulate neuronal activity via tripartite synaptic structures. The precision‐tuning of the function astrocytic is garnering increasing attention because its significance in promoting brain repair following ischemic stroke. Microdomain calcium (Ca 2+ ) transients are pivotal for functional regulation these processes. However, understanding alterations and regulatory mechanism microdomain Ca during stroke remains limited. In present study, a fast high‐resolution, miniaturized two‐photon microscopy used to show that levels significantly reduced peri‐infarct area awake mice. This finding correlated with behavioral deficits shown by mice under freely‐moving conditions. Mitochondrial an important factor driving transients. DEAD Box 1 (DDX1) bound circSCMH1 (a circular RNA involved vascular post‐stroke repair) facilitates formation membrane‐associated RNA‐containing vesicles (MARVs) enhances transients, thereby recovery. These results targeting potential therapeutic approach intervention.

Language: Английский

---

A special issue in Essays in Biochemistry on astrocytes—more than a neuronal support network

Essays in Biochemistry, Journal Year: 2023, Volume and Issue: 67(1), P. 1 - 2

Published: March 1, 2023

Astrocytes are a type of non-neuronal, glial cells, anatomically placed in the intersection between brain blood vessels and other neural cells-including neurons. Such strategic situation confers these cells unique opportunity to sense circulating molecules adapt according different organismal conditions. By acting as sentinel astrocytes thus co-ordinate gene expression profiles, immune responses, signal transduction pathways, metabolic programs that play essential roles formation circuits modulate neurotransmission higher-order functions.

Language: Английский

---

Camk2a suppresses denervated muscle atrophy by maintaining the Ca2+ homeostasis in muscle cells

Cellular and Molecular Biology, Journal Year: 2023, Volume and Issue: 69(11), P. 25 - 29

Published: Nov. 15, 2023

Denervated muscle atrophy is a severe neurological complication that significantly impacts patients' quality of life. Currently, there lack effective treatment methods. This study aims to investigate the molecular mechanisms associated with denervated and explore potential therapeutic targets. In this study, we assessed severity by measuring wet-weight ratio calf muscles. We conducted Western blot immunofluorescence experiments observe morphology cross-sectional area fibers following sciatic nerve transection. Simultaneously, evaluated expression Camk2a in tissue measured changes Ca2+ using BCA method. Additionally, performed HE Sirius Red staining on collagen deposition response overexpression. our observed significant decrease wet weight muscles, myosin, fiber prolonged duration Subsequently, varying degrees elevation levels tissue, while Camk2a, which regulates signal transduction, decreased tissue. Overexpression reduced accumulation resulting higher ratios, larger areas, reduction conclusion, provides first evidence can alleviate calcium overload cells ameliorate atrophy. Our findings suggest may serve as crucial regulatory target

Language: Английский

---

Mitochondrial Ca>sup<2+>/sup< Handling in the Central Nervous System: the role of the mitochondrial Na>sup<+>/sup</Ca>sup<2+>/sup< exchanger (NCLX) in astrocyte metabolism and function

Published: Oct. 26, 2022

I got to know many people, animals, and places that, each in their own way, directly or indirectly, were the reason was able keep moving travel thus far.I met with nothing less than friendship, kindness, support; more ever imagined could deserve.To them, hold my deepest gratitude respect.I have an immeasurable appreciation by guidance example Alicia Kowaltowski has offered me.She very first supervisor when started

Language: Английский

---