Interactions of proteins with heparan sulfate

Essays in Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: April 22, 2024

Abstract Heparan sulfate (HS) is a glycosaminoglycan, polysaccharides that are considered to have arisen in the last common unicellular ancestor of multicellular animals. In this light, large interactome HS and its myriad functions relation regulation cell communication not surprising. The binding proteins determines their localisation diffusion, essential for embryonic development homeostasis. Following biosynthesis initial heparosan polymer, subsequent modifications comprise an established canonical pathway minor pathway. more frequent former starts with N-deacetylation N-sulfation GlcNAc residues, latter C-5 epimerisation GlcA residue adjacent GlcNAc. driven by ionic interactions. multivalent effect arising from many individual bonds between single protein polysaccharide chain results far stronger interaction than would be expected ion-exchange process. instances, upon binding, both parties undergo substantial conformational change, resulting hydrogen van der Waal contributing significant free energy reaction. Nevertheless, dominate protein–polysaccharide kinetically. Together effect, provides explanation observed trapping HS-binding extracellular matrix. Importantly, been dynamic; breaking reforming, while remains bound polysaccharide. These considerations lead model 1D diffusion matrix on HS, involving mechanisms such as sliding, switching rolling.

Language: Английский

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Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium Cobetia marina KMM 1449 and a Genomic Insight into Its Biosynthesis

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(1), P. 29 - 29

Published: Jan. 8, 2025

Some marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number studies indicate significant biological properties individual polysaccharides, such as antiproliferative activity, which makes them promising area for further research. In this study, the capsular polysaccharide (CPS) was obtained from bacterium Cobetia marina KMM 1449, isolated sediment sample collected along shore Sea Japan. The CPS by saline solution, purified series chromatographic procedures, studied chemical methods 1D 2D 1H 13C NMR spectroscopy. following new structure C. 1449 established consisted simultaneously phosphorylated disaccharide repeating units: →4)-α-L-Rhap2S-(1→3)-β-D-Manp6PGro-(1→. To elucidate genetic basis biosynthesis, whole genomic sequence obtained. biosynthetic gene cluster (BGC) about 70 genes composes four regions encoding nucleotide sugar biosynthesis (dTDP-Rha GDP-Man), assembly (GTs genes), translocation (ABC transporter sulfation (PAPS sulfotransferase genes) lipid carrier (wcb operon). Comparative analysis BGCs available genomes showed presence 1449-like BGC among strains all three species. study natural well elucidation pathways their provides development potential anticancer drugs.

Language: Английский

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Effects of the sulfated polysaccharides dextran sulfate and heparin on shrimp immunity and infection by white spot syndrome virus and Vibrio parahaemolyticus

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 42, P. 102735 - 102735

Published: March 11, 2025

Language: Английский

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Chemical approaches to the sulfation of small molecules: current progress and future directions

Essays in Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: July 3, 2024

Abstract Sulfation is one of the most important modifications that occur to a wide range bioactive small molecules including polysaccharides, proteins, flavonoids, and steroids. In turn, these sulfated have significant biological pharmacological roles in diverse processes cell signalling, modulation immune inflammation response, anti-coagulation, anti-atherosclerosis, anti-adhesive properties. This Essay summarises encountered chemical sulfation methods molecules. reactions using sulfur trioxide amine/amide complexes are used method for alcohol phenol groups carbohydrates, steroids, related scaffolds. Despite effectiveness methods, they suffer from issues multiple-purification steps, toxicity (e.g., pyridine contamination), purification challenges, stoichiometric excess reagents which leads an increase reaction cost, intrinsic stability both reagent product. Recent advances SuFEx, situ approach, TBSAB show widespread appeal novel sulfating approaches will enable larger exploration field years come by simplifying isolation process access bespoke

Language: Английский

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Structure and Biosynthetic Gene Cluster of Sulfated Capsular Polysaccharide from the Marine Bacterium Vibrio sp. KMM 8419

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12927 - 12927

Published: Dec. 1, 2024

sp. KMM 8419 (=CB1-14) is a Gram-negative bacterium isolated from food-net mucus sample of marine polychaete

Language: Английский

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Sulfation pathways in times of change

Essays in Biochemistry, Journal Year: 2024, Volume and Issue: 68(4), P. 379 - 382

Published: Dec. 1, 2024

Abstract Sulfation pathways are an essential part of overall sulfur metabolism. mainly about sulfate activation, and the making breaking biological esters. This special issue features some extended reflection on what was presented at SUPA 2023 meeting Pathways. Novel insights into synthesis analytics sulfate, sulfated conjugates, protein persulfides presented. Oxysterol sulfates as emerging sulfo-metabolites. enzymes discussed in various disease settings. also presents polysaccharide sulfotransferases their functional characterization. Finally, cytoplasmic highlighted with regards to impact DNA-modification, context endocrine disruptors. All all, thought-provoking findings, potential guide stimulate future research field sulfation beyond.

Language: Английский

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