Responsively Degradable Nanoarmor‐Assisted Super Resistance and Stable Colonization of Probiotics for Enhanced Inflammation‐Targeted Delivery

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(18)

Published: Jan. 19, 2024

Manipulation of the gut microbiota using oral microecological preparations has shown great promise in treating various inflammatory disorders. However, delivering these while maintaining their disease-site specificity, stability, and therapeutic efficacy is highly challenging due to dynamic changes associated with pathological microenvironments gastrointestinal tract. Herein, a superior armored probiotic an inflammation-targeting capacity developed enhance timely action bacterial therapy against bowel disease (IBD). The coating strategy exhibits suitability for diverse strains negligible influence on viability. This study demonstrates that probiotics have ultraresistance extreme intraluminal conditions stable mucoadhesive capacity. Notably, HA-functionalized nanoarmor equips inflamed-site targetability through multiple interactions, thus enhancing IBD therapy. Moreover, "awakening" ingested responsive transferrin-directed degradation at site inflammation beneficial therapy, which requires cells be fully functional. Given its easy preparation favorable biocompatibility, single-cell approach provides effective advanced delivery biomedical applications cellular level.

Language: Английский

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The gut ecosystem and immune tolerance

Journal of Autoimmunity, Journal Year: 2023, Volume and Issue: 141, P. 103114 - 103114

Published: Sept. 23, 2023

The gastrointestinal tract is home to the largest microbial population in human body. gut microbiota plays significant roles development of immune system and has a substantial impact on maintenance tolerance beginning early life. These microbes interact with dynamic interdependent manner. They generate signals by presenting vast repertoire antigenic determinants metabolites that influence development, maturation immunological function homeostasis. At same time, both innate adaptive systems are involved modulating stable ecosystem between commensal pathogenic microorganisms. Hence, host work together maintain homeostasis synergistically. In susceptible hosts, disruption such harmonious state can greatly affect health lead various auto-inflammatory autoimmune disorders. this review, we discuss our current understanding interactions immunity an emphasis on: a) important players immunity; b) contribution metabolites; c) effect as well alteration microbiome molecular mechanisms driving autoimmunity diseases.

Language: Английский

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A systematic framework for understanding the microbiome in human health and disease: from basic principles to clinical translation

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Sept. 23, 2024

Language: Английский

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Alterations in mucosa branched N -glycans lead to dysbiosis and downregulation of ILC3: a key driver of intestinal inflammation

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 7, 2025

The perturbation of the symbiotic relationship between microbes and intestinal immune system contributes to gut inflammation Inflammatory Bowel Disease (IBD) development. host mucosa glycans (glycocalyx) creates a major biological interface microorganisms immunity that remains ill-defined. Glycans are essential players in IBD immunopathogenesis, even years before disease onset. However, how changes glycosylation shape microbiome this impact response be clarified. Here, we revealed alterations expression complex branched N-glycans at surface, modeled glycoengineered mice, resulted dysbiosis, with deficiency Firmicutes bacteria. Concomitantly, N-glycan switch was associated downregulation type 3 innate lymphoid cells (ILC3)-mediated response, leading transition ILC3 toward an ILC1 proinflammatory phenotype increased TNFα production. In addition, demonstrated remodeling through prophylactic supplementation steady state able restore microbial-derived short-chain fatty acids microbial sensing (by NOD2 expression) alongside rescue module, suppressing controlling complementary approach, further showed patients, often displaying exhibited tendency decreased MGAT5 epithelial accompanied by reduced mucosa. Altogether, these results unlock effects glycome composition regulation bidirectional crosstalk microbiota revealing N-glycans/microbiota/ILC3 axis as pathway homeostasis preventing health transition.

Language: Английский

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Gut commensal Alistipes shahii improves experimental colitis in mice with reduced intestinal epithelial damage and cytokine secretion

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

ABSTRACT The commensal bacterium Alistipes shahii is a core microbe of the human gut microbiome and its abundance negatively correlated with inflammatory bowel diseases (IBDs). However, fundamental role in regulating response remains unknown. Using dextran sulfate sodium (DSS)-induced colitis mouse model, we examined effect A. strain As360 intervention on host found that alleviated disease activity index, colon shortening, colonic histopathological lesion. levels tight junction proteins (mainly ZO1 claudin-1) were decreased DSS-induced mice, whereas these elevated mice treatment. In addition, treatment led to alterations cytokine release, especially an increase IL10. It also reduced expressions mtor Nlrp3 increased expression mTOR inhibitor Ddit4 at transcriptional level. 16S rRNA amplicon sequencing Bacteroides , producer short-chain fatty acids (SCFAs), was enriched fecal samples Metabolic analyses that, following treatment, SCFAs content lactic acid cecal content. These findings suggest supplementation promising strategy prevent colitis. IMPORTANCE As one microbes keystone species gut, has potential inhibit inflammation improve (IBDs) conditions. this study, experimentally demonstrated oral administration symptoms colitis, altered release cellular factors, intestinal epithelial barrier damage, changed microbiota metabolites. provide deeper understanding beneficial effects perspective for better strategies IBD.

Language: Английский

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Oral and Fecal Microbiota as Accurate Non-invasive Tools for Detection of Pancreatic Cancer in the Chinese Population

Cancer Letters, Journal Year: 2025, Volume and Issue: 612, P. 217456 - 217456

Published: Jan. 11, 2025

Language: Английский

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Specific Composition Diets and Improvement of Symptoms of Immune-Mediated Inflammatory Diseases in Adulthood—Could the Comparison Between Diets Be Improved?

Nutrients, Journal Year: 2025, Volume and Issue: 17(3), P. 493 - 493

Published: Jan. 29, 2025

Diet is considered a possible cofactor, which affects the immune system and potentially causes dysregulation of intestinal homeostasis inflammation. This study aimed to review quality evidence on effects specific diet composition symptoms immune-mediated inflammatory diseases (IMIDs), including rheumatoid arthritis (RA), spondyloarthritis, multiple sclerosis (MS), bowel disease (IBD) [remission maintenance Crohn’s ulcerative colitis], psoriasis psoriatic in adult patients. We conducted meta-analyses Cochrane systematic reviews using PubMed EMBASE, from inception September 2024, Google Scholar. The methodological was assessed AMSTAR 2 rating system. Three eight were evaluated. Some diets have been shown reduce RA, IBD, MS improve activity parameters IBD with critically low or levels evidence. reduction biomarker unclear. summarizes global for dietary interventions, mostly anti-inflammatory properties due their components, IMID symptoms, clarifying weaknesses clinical trials evidence; shows need use indices such as Dietary Inflammatory Index, allows be classified by pro-inflammatory food content, better compare groups trials. difficulty obtaining high-level studies apparent may delay application results. Clinicians should aware role complement pharmacological treatments IMIDs.

Language: Английский

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The Role of the Gut Microbiome in Immune Dysregulation and Pathogenesis of Inflammatory Bowel Disease

Developmental medico-life-sciences, Journal Year: 2025, Volume and Issue: 2(1), P. 13 - 25

Published: Jan. 31, 2025

Background: Inflammatory Bowel Disease (IBD), encompassing Crohn's (CD) and Ulcerative Colitis (UC), is a chronic, debilitating disorder affecting the gastrointestinal tract. The gut microbiome pivotal in maintaining intestinal homeostasis regulating immune function. Dysbiosis, or microbial imbalance, has been increasingly recognized as key factor pathogenesis of IBD, driving chronic inflammation dysregulation. Objectives: This systematic review aims to explore relationship between responses IBD. Specifically, it investigates how dysbiosis contributes disease modulation, evaluates efficacy microbiome-targeted therapies such probiotics, prebiotics, fecal microbiota transplantation (FMT). Methods: We conducted comprehensive search PubMed, Scopus, Web Science for studies published 2000 2024. Studies included randomized controlled trials, observational studies, reviews focused on alterations IBD use interventions. Quality was assessed using Cochrane Risk Bias Tool Newcastle-Ottawa Scale. Data synthesis performed narrative analysis descriptive statistics. Results: Key findings indicate that marked by reduction beneficial taxa Faecalibacterium prausnitzii Akkermansia muciniphila, alongside overgrowth pathogenic microbes like Escherichia coli (AIEC). Microbiome-targeted therapies, including FMT, showed promising results restoring balance, though variable, particularly UC CD. Conclusion: Dysbiosis central pathogenesis. offer potential but require personalized approaches improve treatment efficacy. Future research should integrate multi-omics technologies better understanding management

Language: Английский

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Harnessing Akkermansia muciniphila Membrane Coating for Probiotic Therapy in Inflammatory Bowel Disease

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Inflammatory bowel diseases (IBDs) are characterized by dysregulated inflammatory responses, increased reactive oxygen species (ROS), and compromised intestinal barrier function. Current clinical treatments often yield suboptimal responses significant side effects. Given the great potential of Akkermansia muciniphila (AKK) bacterial membrane components in IBD treatment possible pathogenicity live bacteria, to essentially address these challenges, we develop a natural membrane-based single-cell coating, incorporating self-assembled AKK (AKM) astaxanthin (AST), forming protective biological on probiotics. Utilizing Escherichia coli Nissle 1917 (EcN) for oral delivery system, engineer AKM-AST@EcN nanocoated probiotics, demonstrating exceptional gastrointestinal (GI) tract tolerance robust bioactivity. Critically, our findings highlight as promising therapeutic approach while also presenting distinctive strategy probiotic systems.

Language: Английский

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Exploring the Role of Gut Microbiota and Probiotics in Acute Pancreatitis: A Comprehensive Review

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3433 - 3433

Published: April 6, 2025

Acute pancreatitis (AP) is a common and potentially severe gastrointestinal condition characterized by acute inflammation of the pancreas. The pathophysiology AP multifactorial intricate, involving cascade events that lead to pancreatic injury systemic inflammation. progression influenced many factors, including genetic predispositions, environmental triggers, immune dysregulation. Recent studies showed critical involvement gut microbiota in shaping response modulating inflammatory processes during AP. This review aims provide comprehensive overview emerging role probiotics We analyzed implication pathogenesis modification an attack. primary goals microbiome-based therapies, which include probiotics, prebiotics, antibiotics, fecal transplantation, enteral nutrition, are alter composition microbial community amount metabolites derived from microbiota. By resetting entire flora or supplementing it with certain beneficial organisms their byproducts, these therapeutic approaches aim eradicate harmful microorganisms, reducing avoiding bacterial translocation potential microbiota-based target for nutrition pre- probiotic supplementation transplantation.

Language: Английский

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