Seminars in Hematology, Journal Year: 2024, Volume and Issue: 61(2), P. 69 - 72
Published: March 26, 2024
Language: Английский
Leukemia & lymphoma/Leukemia and lymphoma, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13
Published: Jan. 2, 2025
In this review, we focus on the pro-oncogene MYC, modes of deregulation in mouse and human B-cells, its undisputable importance evaluation biological prognostication patients, but also how it impacts response to modern therapeutics, should be targeted improve overall survival chronic lymphocytic lymphoma (CLL) patients. After an overview current understanding molecular dysregulation c-MYC, will show CLL, both indolent transformed phases, has developed among other B-cell lymphomas a tight regulation expression through activation B-Cell Receptors (among others). This is particularly important if one desires understand mechanisms at stake over-expression c-MYC especially lymph nodes compartment. So doing, oncogene orchestrates pivotal cellular functions such as metabolism, drug resistance, proliferation histologic transformation (Richter syndrome).
Language: Английский
Citations
0
Leukemia & lymphoma/Leukemia and lymphoma, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11
Published: Jan. 7, 2025
Prognostic assessment in chronic lymphocytic leukemia (CLL) is essential for delivery of timely, personalized therapy. TP53 status, karyotype, IGHV mutational minimal residual disease (MRD), gene mutations and markers cell proliferation were important prognostic tools the era chemo-immunotherapy (CIT). With BCL2 inhibitors (BCL2i), outcome still impacted by complex achievement undetectable MRD. On other hand, BTK (BTKi) are agnostic to rarely cause MRD negative remissions less clearly status. Although based on mature data, outcomes with BCL2i/BTKi combinations likely influenced Responses non-covalent BTKI (ncBTKI) mechanism resistance previous covalent BTKi. Finally, responses chimeric antigen receptor T therapy (CAR-T) appear independent but dependent overall T- fitness.
Language: Английский
Citations
0
Cancers, Journal Year: 2025, Volume and Issue: 17(6), P. 964 - 964
Published: March 13, 2025
Background/Objectives: Massively parallel sequencing technologies have advanced chronic lymphocytic leukemia (CLL) diagnostics and precision oncology. Illumina platforms, while offering robust performance, require substantial infrastructure investment a large number of samples for cost-efficiency. Conversely, third-generation long-read nanopore from Oxford Nanopore Technologies (ONT) can significantly reduce costs, making it valuable tool in resource-limited settings. However, faces challenges with lower accuracy throughput than necessitating additional computational strategies. In this paper, we demonstrate that integrating publicly available short-read data in-house generated ONT data, along the application machine learning approaches, enables characterization CLL transcriptome landscape, identification clinically relevant molecular subtypes, assignment these subtypes to nanopore-sequenced samples. Methods: Public RNA 608 were obtained CLL-Map Portal. analysis, gene module identification, transcriptomic subtype classification performed using oposSOM R package high-dimensional visualization self-organizing maps. Eight patients recruited Hematology Center After Prof. R. Yeolyan (Yerevan, Armenia). Sequencing libraries prepared blood total PCR-cDNA sequencing-barcoding kit (SQK-PCB109) following manufacturer’s protocol sequenced on an R9.4.1 flow cell 24–48 h. Raw reads converted TPM values. These projected into SOMs space supervised portrayal (supSOM) approach predict portrait new support vector regression. Results: The landscape reveals disruptions modules (spots) associated T cytotoxicity, B activation, inflammation, cycle, DNA repair, proliferation, splicing. A specific contained genes poor prognosis CLL. Accordingly, classified T-cell cytotoxic, immune, proliferative, splicing, three mixed types: proliferative–immune, proliferative–splicing, proliferative–immune–splicing. survival orthogonal gender mutation status. Using assigned patient sequencing. Conclusions: This study demonstrates be parsed functional modules, revealing distinct based proliferative immune activity, important implications treatment are other classifications. Additionally, integration public datasets offers cost-effective subtyping prognostic prediction, facilitating more accessible personalized care.
Language: Английский
Citations
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Hematology Reports, Journal Year: 2025, Volume and Issue: 17(2), P. 18 - 18
Published: March 28, 2025
Leukemia is a heterogeneous group of hematologic malignancies characterized by distinct genetic and molecular abnormalities. Advancements in genomic technologies have significantly transformed the diagnosis, prognosis, treatment strategies for leukemia. Among these, next-generation sequencing (NGS) has emerged as powerful tool, enabling high-resolution profiling that surpasses conventional diagnostic approaches. By providing comprehensive insights into mutations, clonal evolution, resistance mechanisms, NGS revolutionized precision medicine leukemia management. Despite its transformative potential, clinical integration presents challenges, including data interpretation complexities, standardization issues, cost considerations. However, continuous advancements platforms bioinformatics pipelines are enhancing reliability accessibility routine practice. The expanding role paving way improved risk stratification, targeted therapies, real-time disease monitoring, ultimately leading to better patient outcomes. This review highlights impact on research applications, discussing advantages over traditional techniques, key approaches, emerging challenges. As oncology continues evolve, expected play an increasingly central diagnosis management leukemia, driving innovations personalized therapeutic interventions.
Language: Английский
Citations
0
Seminars in Hematology, Journal Year: 2024, Volume and Issue: 61(2), P. 69 - 72
Published: March 26, 2024
Language: Английский
Citations
0