The lipid droplet lipidome

FEBS Letters, Journal Year: 2024, Volume and Issue: 598(10), P. 1215 - 1225

Published: April 11, 2024

Lipid droplets (LDs) are intracellular organelles with a hydrophobic core formed by neutral lipids surrounded phospholipid monolayer harboring variety of regulatory and enzymatically active proteins. Over the last few decades, our understanding LD biology has evolved significantly. Nowadays, LDs appreciated not just as passive energy storage units, but rather players in regulation lipid metabolism quality control machineries. To fulfill their functions controlling cellular metabolic states, need to be highly dynamic responsive organelles. A large body evidence supports nature proteome its contact sites other However, much less is known about lipidome LDs. Numerous examples clearly indicate intrinsic link between proteins, calling for deeper characterization various physiological pathological settings. Here, we reviewed current state knowledge field lipidome, providing brief overview classes molecular species present within monolayer.

Language: Английский

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Recent progress in small molecule fluorescent probes for imaging and diagnosis of non-alcoholic fatty liver disease

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 513, P. 215864 - 215864

Published: May 7, 2024

Language: Английский

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Mitochondrial dysfunction in drug-induced hepatic steatosis: recent findings and current concept

Clinics and Research in Hepatology and Gastroenterology, Journal Year: 2025, Volume and Issue: 49(3), P. 102529 - 102529

Published: Jan. 9, 2025

Language: Английский

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Metabolic dysfunction–associated steatotic liver disease and the gut microbiome: pathogenic insights and therapeutic innovations

Journal of Clinical Investigation, Journal Year: 2025, Volume and Issue: 135(7)

Published: March 31, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a major cause of worldwide, and our understanding its pathogenesis continues to evolve. MASLD progresses from steatosis steatohepatitis, fibrosis, cirrhosis, this Review explores how the gut microbiome their metabolites contribute pathogenesis. We explore complexity importance intestinal barrier function disruptions dysbiosis work in concert promote onset progression MASLD. The focuses on specific bacterial, viral, fungal communities that impact trajectory (including ethanol, bile acids, short chain fatty other metabolites) Finally, we underscore knowledge interaction between microbes may be leveraged for microbial-based therapeutics. Here, include discussion therapeutic potential prebiotics, probiotics, postbiotics, microbial-derived metabolites.

Language: Английский

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The global prevalence and impact of steatotic liver disease and viral infections: A systematic review and meta-analysis

Hepatology Communications, Journal Year: 2025, Volume and Issue: 9(5)

Published: April 14, 2025

Background: Steatotic liver disease (SLD) affects ~30% of adults worldwide. The global population is continuously threatened by epidemic and endemic viral diseases. This study aims to thoroughly examine the interaction between SLD major Methods: We systematically searched databases from inception April 2, 2024, for observational studies recording viral-infected adult patients with eligible data on presence hepatic steatosis. Results: Six hundred thirty-six were included in analysis prevalence. Among monoinfections, highest prevalence was observed those infected HCV at 49% (95% CI: 47%–51%), followed SARS-CoV-2 (39%, 95% CI [34%–44%]), HIV [33%–44%]), HBV (36%, [32%–40%]). Additionally, co-infections, such as HCV-HIV HBV-HCV, exhibit even higher steatohepatitis particularly high HIV-infected (24%, 17%–30%) HCV-infected (18%, 13%–24%) populations. co-existence infections associated not only progression but also more severe outcomes poorer responses antiviral treatment. combination cardiometabolic risk factors viral-associated host contributes Conclusions: highly prevalent populations, reciprocal interactions diseases exacerbate both conditions, leading patient general.

Language: Английский

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A triphenylamine-based fluorescent probe with large Stokes shift for wash-free imaging of lipid droplets and diagnosis of fatty liver

New Journal of Chemistry, Journal Year: 2024, Volume and Issue: 48(23), P. 10427 - 10431

Published: Jan. 1, 2024

A D–π–A structural probe was synthesized, which has been validated for distinguishing between fatty liver and normal at the organ levels.

Language: Английский

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New insights into lipid and fatty acid metabolism from Raman spectroscopy.

The Analyst, Journal Year: 2024, Volume and Issue: 149(19), P. 4789 - 4810

Published: Jan. 1, 2024

One of the challenges facing biology is to understand metabolic events at a single cellular level. While approaches examine dynamics protein distribution or report on spatiotemporal location signalling molecules are well-established, tools for dissection metabolism in living cells less common. Advances Raman spectroscopy, such as stimulated scattering (SRS), beginning offer new insights into range experimental systems, including model organisms and clinical samples, across disciplines. Despite power imaging, it remains relatively under-used technique approach biological problems, part because specialised nature analysis. To raise profile this method, here we consider some key studies which illustrate how spectroscopy has revealed fatty acid lipid systems. The powerful non-invasive offers suite biomolecular scientists address within informs underpins function. We potential applications, discuss recent advances, direction travel research area.

Language: Английский

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Silencing of Hepatic AATF Ameliorates Metabolic Dysfunction Associated Steatohepatitis by Promoting Fatty Acid β-Oxidation in Experimental models

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 25, 2024

ABSTRACT Background Metabolic dysfunction-associated steatotic liver disease (MASLD) affects 30% of the global population and has emerged as a significant public health concern. It is multifactorial pathological condition driven by various molecular drivers. Thus, identifying understanding drivers involved in MASLD pathogenesis essential for developing targeted therapeutic strategies. Aim To elucidate role apoptosis antagonizing transcription factor (AATF) driver its underlying mechanisms. Methods A preclinical murine model resembling human was employed, which C57Bl/6 mice were fed either chow diet with normal water (CD) or western sugar (WD). After 12 weeks, injected via tail vein AAV8 serotype particles randomized into four groups: (i) CD AAV8-TBG-Null, (ii) AAV8-TBG-siAATF, (iii) WD AAV8-TBG-Null (WD control), (iv) AAV8-TBG-siAATF siAATF). Biochemical, histological, analyses performed using serum tissues. Untargeted metabolomics carried out tissues experimental MASLD. Results AATF upregulated cellular models Liver-specific silencing reduced body weight, insulin resistance compared to controls but had no effect on mice. showed injury, steatosis, inflammation, fibrosis AATF-silenced Furthermore, untargeted revealed increased levels glycerophospholipids, such phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines, siAATF Consistently, expression hepatic carnitine palmitoyl transferase (CPT1) peroxisome proliferator-activated receptor alpha/gamma (PPAR α/γ), promote fatty acid beta oxidation, controls. Conclusion These findings show link between lipid metabolism, implying that could be promising target

Language: Английский

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Natural molecule isoliquiritigenin mitigates MASH and liver fibrosis in mice by promoting autophagy through the PI3K/Akt signaling pathway

The Journal of Nutritional Biochemistry, Journal Year: 2024, Volume and Issue: unknown, P. 109808 - 109808

Published: Nov. 1, 2024

Language: Английский

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