OBM Geriatrics, Journal Year: 2025, Volume and Issue: 09(02), P. 1 - 32
Published: April 14, 2025
Cellular senescence (aging) is a physiological process that plays role in tissue remodeling, wound healing, and embryogenesis. Prolonged of cells can have detrimental effects trigger secretory phenotype (SASP, senescence-associated phenotype), degenerative disorders, cancer, age-related diseases. Suitable biomarkers range different laboratory methods are used to investigate these complex relationships vitro vivo. Since universal biomarker for cell has not yet been identified, numerous identify senescent cell. The detection quantification their SASP provide the basis targeted treatment patient. In parallel, single-cell analysis also required quantitative assessment therapy result. Depending on facilities performing analysis, wide available. this review, we general overview accessible techniques such as immunohistochemistry using microscopy automated flow cytometry introduce new possibilities by modern like mass spectrometry or genetic method single cells. focus here use routine laboratories. classical with enzyme immunoassays, measurement products (IL-6, IL-8), part work. This review discusses ideas visualisation clinical patient data gerontology. An outlook potential future optimization improve rejuvenate status patients cellular organ-specific level discussed.
Language: Английский