Biomimetic fabrication bioprinting strategies based on decellularized extracellular matrix for musculoskeletal tissue regeneration: Current status and future perspectives

Materials & Design, Journal Year: 2024, Volume and Issue: 243, P. 113072 - 113072

Published: June 6, 2024

Musculoskeletal disorders, as one of the prevalent categories ailments, exert significant impacts on individuals' lives, occupations, and physical activities. Degenerative changes, injuries, infections, tumor resections causing defects in musculoskeletal tissues such cartilage, bones, skeletal muscles, menisci, ligaments, rotator cuffs can detrimentally affect patients' quality life mental well-being. Traditional autologous allogeneic transplantations have been clinically employed. However, transplantation suffers from limitation a finite number transplantable tissues, while faces challenges immune rejection. The extracellular matrix (ECM) serves natural scaffold for cells to fulfill physiological functions adhesion, proliferation, differentiation. Decellularized (dECM) emerges promising biomaterial generated through specific tissue or organ decellularization. Leveraging 3D bioprinting technology, dECM-based biomaterials enable customized printing construction. This study reviews various decellularization techniques, post-decellularization strategies, commonly used technologies. It summarizes integration with technology applied system research. These investigations showcase exciting potential system, offering prospects clinical translation orthopedics.

Language: Английский

Comb-assisted 3D bioprinting for highly aligned 3D muscle bioconstructs with enhanced cellular mechanotransduction

Virtual and Physical Prototyping, Journal Year: 2025, Volume and Issue: 20(1)

Published: May 5, 2025

Language: Английский

Decellularization of fish tissues for tissue engineering and regenerative medicine applications

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 28, 2024

Abstract Decellularization is the process of obtaining acellular tissues with low immunogenic cellular components from animals or plants while maximizing retention native extracellular matrix structure, mechanical integrity and bioactivity. The decellularized tissue obtained through decellularization technique retains structure bioactive its tissue; it not only exhibits comparatively strong properties, immunogenicity good biocompatibility but also stimulates in situ neovascularization at implantation site regulates polarization recruited macrophages, thereby promoting regeneration damaged tissue. Consequently, many commercial products have been developed as promising therapeutic strategies for treatment different defects lesions, such wounds, dura, bone cartilage defects, nerve injuries, myocardial infarction, urethral strictures, corneal blindness other orthopedic applications. Recently, there has a growing interest fish because abundance sources, less religious constraints risks zoonosis transmission between mammals. In this review, we provide complete overview state-of-the-art tissues, including organs methods used to prepare tissues. We enumerated common various organs, skin, scale, bladder, cartilage, heart brain, elaborated their processing engineering Furthermore, presented perspectives (i) future development direction technology, (ii) expanding sources (iii) innovating bio-inks 3D bioprinting unleash great potential regenerative medicine

Language: Английский