Terahertz wave alleviates comorbidity anxiety in pain by reducing the binding capacity of nanostructured glutamate molecules to GluA2

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

Comorbid anxiety in chronic pain is clinically common, with a comorbidity rate of over 50%. The main treatments are based on pharmacological, interventional, and implantable approaches, which have limited efficacy carry risk side effects. Here, we report terahertz (THz, 10 12 Hz) wave stimulation (THS) technique, exerts nonthermal, long-term modulatory effects neuronal activity by reducing the binding between nano-sized glutamate molecules GluA2, leading to relief comorbid anxiety-like behaviors mice. In mice co-occurring induced complete Freund’s adjuvant (CFA) injection, hyperactivity was observed glutamatergic neurons anterior cingulate cortex (ACC Glu ). Using whole-cell recording ACC slices, demonstrated that THS (34 THz) effectively inhibited excitability . Moreover, molecular dynamics simulations showed reduced number hydrogen bonds bound GluA2. Furthermore, target CFA-treatment suppressed and, as result, alleviated behaviors. Consistently, inhibition chemogenetics mimics THS-induced antinociceptive antianxiety behavior. Together, our study provides evidence for an intervention technique modulating viable clinical treatment strategy anxiety.

Language: Английский

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Interaction networks within biomolecular condensates feature topological cliques near the interface

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Biomolecular condensates are typically maintained by networks of molecular interactions, with canonical examples including those formed prion-like low complexity domains (LCDs) proteins. Single-component LCD have been predicted to exhibit small-world network topologies and spatial inhomogeneities in protein compaction. Here, we systematically characterize underlying investigate the relationship between single molecule properties topologies. We employ a chemically specific coarse-grained model probe generalize our findings varying sequence hydrophobicity via generic that describes "hydrophobic-polar" (HP) polymers. For both systems, find sustained featuring "hubs" "cliques". Molecular hubs high betweenness centrality localize near centers adopt more elongated conformations. In contrast, cliques-densely interacting molecules form locally fully connected subgraphs-are bridged tend condensate interface. Interestingly, power-law relationships structure dynamics individual centrality, which connectivity. Thus, work demonstrates connectivity can be from single-molecule properties. Furthermore, cliques longer lifetimes their constituent remain spatially constrained, suggesting role shaping interface material

Language: Английский

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Sequence-dependent biomolecular phase separation driven by short-range interaction: From material properties to coarsening dynamics

Physical review. E, Journal Year: 2025, Volume and Issue: 111(5)

Published: May 8, 2025

Language: Английский

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Interfacial Enrichment of Lauric Acid Assisted by Long-Chain Fatty Acids, Acidity and Salinity at Sea Spray Aerosol Surfaces

The Journal of Physical Chemistry A, Journal Year: 2024, Volume and Issue: 128(34), P. 7195 - 7207

Published: Aug. 6, 2024

Surfactant monolayers at sea spray aerosol (SSA) surfaces regulate various atmospheric processes including gas transfer, cloud interactions, and radiative properties. Most experimental studies of SSA employ a simplified surfactant mixture long-chain fatty acids (LCFAs) as proxy for the surface microlayer or surface. However, medium-chain (MCFAs) make up nearly 30% FA fraction in nascent SSA. Given that LCFA are easily disrupted upon introduction chemical heterogeneity (such mixed chain lengths), simple proxies unlikely to represent realistic interfaces. Integrating computational techniques, we characterize impact partially soluble MCFAs have on properties atmospherically relevant mixtures. We explore extent which MCFA lauric acid (LA) is stabilized by varying acidity, salinity, monolayer composition. also discuss impacts pH LCFA-assisted LA retention, where presence LCFAs may shift surface-adsorption equilibria laurate─the conjugate base─toward higher activities. Molecular dynamic simulations suggest mechanism enhanced retention laurate. conclude increased promotes activity species, altering phase behavior impacting climate-relevant processes.

Language: Английский

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