Optics & Laser Technology, Journal Year: 2024, Volume and Issue: 182, P. 112028 - 112028
Published: Nov. 26, 2024
Language: Английский
Biomedical Optics Express, Journal Year: 2025, Volume and Issue: 16(2), P. 837 - 837
Published: Jan. 15, 2025
Optical diffraction tomography (ODT) is an important technique for three-dimensional (3D) imaging of semi-transparent biological samples, enabling volumetric visualization living cells, cultures, and tissues without the need exogenous dyes. However, ODT faces significant challenges in complex specimens due to limited specificity refractive index (RI) coupled relationship between absorption phase image formation. Here, we present multi-modal transport intensity (MM-TIDT), a high-speed 3D microscopy that integrates electrically tunable lens with modified illumination patterns decouple information. Leveraging dual schemes-circular annular apertures-MM-TIDT acquires two stacks, facilitating accurate decoupling. Based on alternating direction method multipliers (ADMM) framework total variation (TV) non-negativity regularization, our reconstructs distributions fluorescence RI high accuracy robustness. Experimental validation fluorescent polystyrene microspheres, Spirulina specimens, DAPI-labeled C166 cells demonstrates capability MM-TIDT resolve fine structural details across diverse sample types, providing versatile platform exploring dynamic processes intricate cellular interactions.
Language: Английский
Citations
1
Optics Letters, Journal Year: 2025, Volume and Issue: 50(6), P. 1909 - 1909
Published: Feb. 12, 2025
In this work, a new framework of lensless Fourier transform multiplexed digital holography (LFTMDH) is proposed, leveraging the double field view (FoV) in single-shot recording. The proof-of-concept system with FoV realized by adopting spatially multiplexing technique inserting cube beam splitter object path. divides into two FoVs different information. By carefully aligning and optimizing orientation splitter, these along spherical reference (generated at plane) are allowed to interfere active region an image sensor, resulting hologram. information (amplitude phase) obtained applying single recorded hologram, making faster added benefit FoV, compared its counterparts. feasibility advantages proposed demonstrated through experimental validation comparative analysis existing system. results indicate that not only achieves imaging recording but also maintains spatial resolution reconstructed images. Its applications span various fields, such as biological microscopy, nondestructive testing, optical metrology, where wider crucial.
Language: Английский
Citations
0
Applied Physics Letters, Journal Year: 2024, Volume and Issue: 125(13)
Published: Sept. 23, 2024
Lensless in-line holography is widely used to obtain depth information on pathological tissues and biological cells enable noninvasive analysis, due low cost large field-of-view. However, existing systems suffer from crosstalk dilemma of twin-image that limit their capability achieve high-resolution phase imaging with a single measurement, especially in the presence noise. In this Letter, we propose physics-guided frequency-aware network (FA-PGNet) for single-shot lens-free holographic reconstruction. The FA-PGNet method uses frequency-level supervision balance high- low-frequency between images gradually obtained reliability results by incorporating physical model. With co-addition physics-guided, it can easily tackle speckle noise, 5.88 dB improvement peak signal-to-noise ratio 6-fold reduction uncertainty system. Optical experiments demonstrate allows access complex features microscopic high resolution. We believe proposed provides powerful tool cell imaging.
Language: Английский
Citations
2
Optics Express, Journal Year: 2024, Volume and Issue: 32(17), P. 29329 - 29329
Published: July 23, 2024
We present a portable single-shot lens-free tomographic microscope, based on spatial multiplexing in-line digital holography, for three-dimensional (3D) imaging of dynamic specimens. The simplified system is realized by only laser diodes array, rectangular aperture, and CMOS image sensor with global shutter, which enables the recording all complex fields specimen from different illumination angles without any scanning mechanism hologram, in single camera exposure. Using our proposed data processing method, high-quality 3D tomograms, lateral resolution 3.36μm an axial 6.39μm within field-of-view 5.06 mm
Language: Английский
Citations
0
Optics Express, Journal Year: 2024, Volume and Issue: 32(18), P. 31920 - 31920
Published: Aug. 19, 2024
Digital holography can reconstruct the amplitude and phase information of target light field. However, reconstruction quality is largely limited by size hologram. Multi-plane holograms impose constraints for reconstruction, yet reconstructed images continues to be restricted owing deficiency effective prior constraints. To attain high-quality image a diffusion model-boosted multiplane extrapolation digital holographic (DMEDH) algorithm proposed. The dual-channel extracted through denoising score matching employed constrain physically driven dual-domain rotational iterative process. Depending on utilization multi-plane hologram data, serial DMEDH parallel are presented. Compared with traditional methods, simulative experimental results demonstrate that using exhibit better have higher structural similarity, peak signal-to-noise ratios, strong generalization. from two exhibits than methods five holograms.
Language: Английский
Citations
0
Infrared and Laser Engineering, Journal Year: 2024, Volume and Issue: 53(9), P. 20240348 - 20240348
Published: Jan. 1, 2024
Citations
0
ACTA PHOTONICA SINICA, Journal Year: 2024, Volume and Issue: 53(9), P. 0911001 - 0911001
Published: Jan. 1, 2024
Citations
0
Optics & Laser Technology, Journal Year: 2024, Volume and Issue: 182, P. 112028 - 112028
Published: Nov. 26, 2024
Language: Английский
Citations
0