
ACS Omega, Journal Year: 2025, Volume and Issue: 10(5), P. 4860 - 4869
Published: Jan. 29, 2025
This study evaluated the ethanol–water modified (50%, v/v) supercritical carbon dioxide (SC-CO2) for extraction of polyphenols from muscadine grape (Vitis rotundifolia Michgx.) pomace and compared with conventional solvent extractions (ethanol–water HCl–methanol). The process was optimized a central composite response surface design consisting three levels independent variables: pressure (20–40 MPa), temperature (40–60 °C), cosolvent concentration (5–15%) to maximize responses: total phenolic content (TPC), flavonoid (TFC), resveratrol yields. optimal conditions were determined as 20 MPa, 60 °C, 15% TPC, TFC, yields 2491 mg/100 g, 674 1.07 respectively. plots indicated that maximized efficiency, producing red-brown colored extracts. In contrast, 5% resulted in poor extractions, yielding yellow-green extracts under all conditions. increased higher temperatures (i.e., °C) lower pressures MPa). TPC TFC obtained through cosolvent-modified SC-CO2 similar those extractions. Moreover, yield than HCl–methanol extraction, even though it not different any at solvent-to-solute ratios. analysis antioxidants ABTS values extract extract. However, there no significant differences DPPH between two Thus, this sustainable technology by employing only food-grade cosolvents─ethanol water─as more environmentally friendly method isolating underutilized waste product utilizing statistical methodologies process.
Language: Английский