Stem cell mechanoadaptation. II. Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling DOI Creative Commons
Vina D. L. Putra, K. Kilian, Melissa L. Knothe Tate

et al.

APL Bioengineering, Journal Year: 2025, Volume and Issue: 9(1)

Published: Jan. 7, 2025

Stem cells adapt to their local mechanical environment by rearranging cytoskeleton, which underpins the evolution of shape and fate as well emergence tissue structure function. Here, in second part a two-part experimental series, we aimed elucidate spatiotemporal cytoskeletal remodeling resulting changes morphology properties nuclei. Akin testing most basic living adapting unit life, i.e., cell, situ model templates, probed native microtubule-stabilized (via Paclitaxel, PAX, exposure) stem cells' adaptation capacity on substrates increasing compliance (exerting tension cells) with increased target seeding densities compression cells). On 10 100 kPa gels, seeded at 5000 cells/cm2 proliferated 15 000 exhibited bulk moduli that nearly matched those respective substrates; hence, they greater increase Young's Modulus after microtubule stabilization than cultured glass. Culture compliant also reduced F-actin, concentration increases compared F-actin alignment decreased more randomly oriented, short actin crosslinks were observed, representing emergent substrate, mediated through myosin II contractility. We conclude cell facilitates accommodation larger loads from PAX-stabilized polymerizing microtubule, which, turn, exerts effect determining stiffen remodel cytoskeleton. Taken whole, these studies establish correlations between cytoskeleton physical parameters cells. Hence, progress our understanding dynamic nuclei, culminating development healing.

Language: Английский

Stem cell mechanoadaptation. II. Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling DOI Creative Commons
Vina D. L. Putra, K. Kilian, Melissa L. Knothe Tate

et al.

APL Bioengineering, Journal Year: 2025, Volume and Issue: 9(1)

Published: Jan. 7, 2025

Stem cells adapt to their local mechanical environment by rearranging cytoskeleton, which underpins the evolution of shape and fate as well emergence tissue structure function. Here, in second part a two-part experimental series, we aimed elucidate spatiotemporal cytoskeletal remodeling resulting changes morphology properties nuclei. Akin testing most basic living adapting unit life, i.e., cell, situ model templates, probed native microtubule-stabilized (via Paclitaxel, PAX, exposure) stem cells' adaptation capacity on substrates increasing compliance (exerting tension cells) with increased target seeding densities compression cells). On 10 100 kPa gels, seeded at 5000 cells/cm2 proliferated 15 000 exhibited bulk moduli that nearly matched those respective substrates; hence, they greater increase Young's Modulus after microtubule stabilization than cultured glass. Culture compliant also reduced F-actin, concentration increases compared F-actin alignment decreased more randomly oriented, short actin crosslinks were observed, representing emergent substrate, mediated through myosin II contractility. We conclude cell facilitates accommodation larger loads from PAX-stabilized polymerizing microtubule, which, turn, exerts effect determining stiffen remodel cytoskeleton. Taken whole, these studies establish correlations between cytoskeleton physical parameters cells. Hence, progress our understanding dynamic nuclei, culminating development healing.

Language: Английский

Citations

2