Vestigial mediates the effect of insulin signaling pathway on wing-morph switching in planthoppers

PLoS Genetics, Journal Year: 2021, Volume and Issue: 17(2), P. e1009312 - e1009312

Published: Feb. 9, 2021

Wing polymorphism is an evolutionary feature found in a wide variety of insects, which offers model system for studying the significance dispersal. In wing-dimorphic planthopper Nilaparvata lugens , insulin/insulin-like growth factor signaling (IIS) pathway acts as ‘master signal’ that directs development either long-winged (LW) or short-winged (SW) morphs via regulation activity Forkhead transcription subgroup O ( Nl FoxO). However, downstream effectors IIS–FoxO cascade mediate alternative wing are unclear. Here we vestigial Nlvg ), key wing-patterning gene, selectively and temporally regulated by during wing-morph decision stage (fifth-instar stage). RNA interference (RNAi)-mediated silencing Nlfoxo increase expression fifth-instar (the last nymphal stage), thereby inducing LW development. Conversely, can antagonize effects IIS on development, redirecting commitment from to morph with intermediate size. vitro vivo binding assays indicated FoxO protein may suppress directly first intron region locus. Our findings provide glimpse link connecting network developmental plasticity wings help us understanding how phenotypic diversity generated modification common set pattern elements.

Language: Английский

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Interorgan communication through peripherally derived peptide hormones in Drosophila

Fly, Journal Year: 2022, Volume and Issue: 16(1), P. 152 - 176

Published: May 1, 2022

In multicellular organisms, endocrine factors such as hormones and cytokines regulate development homoeostasis through communication between different organs. For understanding interorgan communications factors, the fruit fly Drosophila melanogaster serves an excellent model system due to conservation of essential systems flies mammals availability powerful genetic tools. other insects, functions neuropeptides or peptide from central nervous have been extensively studied. However, a series recent studies conducted in revealed that derived peripheral tissues also play critical roles regulating multiple biological processes, including growth, metabolism, reproduction, behaviour. Here, we summarise advances target organs/tissues peripherally describe how these contribute various events communications.

Language: Английский

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Circadian clock regulates developmental time through ecdysone and juvenile hormones in Bombyx mori

Insect Molecular Biology, Journal Year: 2023, Volume and Issue: 32(4), P. 352 - 362

Published: Feb. 23, 2023

Abstract The circadian clock plays an integral role in hormone biosynthesis and secretion. However, how the precisely coordinates hormonal homeostasis to maintain normal animal development remains unclear. Here, we show that knocking out core gene Cryptochrome 1 ( Cry1 ) significantly delays developmental time Bombyx mori . This study focuses on ecdysone juvenile signalling pathways of fifth instar larvae with longest delay. We found mutant reduced prothoracicotropic synthesis brain, could not produce sufficient prothoracic gland, resulting a delayed peak 20‐hydroxyecdysone titre hemolymph larvae, prolonging time. Moreover, further investigation revealed enhanced pathway this higher also resulted prolonged larvae. Our results provide insights into molecular mechanisms by which regulates maintaining homeostasis.

Language: Английский

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Myoglianin triggers the premetamorphosis stage in hemimetabolan insects

The FASEB Journal, Journal Year: 2018, Volume and Issue: 33(3), P. 3659 - 3669

Published: Nov. 27, 2018

Insect metamorphosis is triggered by a decrease in juvenile hormone (JH) the final instar. What induces this therefore relevant question. Working with cockroach Blattella germanica, we found that myoglianin (Myo), ligand TGF-β signaling pathway, highly expressed corpora allata (CA, JH-producing glands) and prothoracic gland [(PG), which produce ecdysone] during penultimate (fifth) nymphal instar (N5). In CA, high Myo levels N5 repress expression of acid methyl transferase, JH biosynthesis gene. PG, decreasing trigger degeneration, regulated factors Krüppel homolog 1, FTZ-F1, E93, inhibitor apoptosis-1. Also peak myo indirectly stimulates ecdysone genes, such as neverland, enhancing production metamorphic pulse N6. The also represses cell proliferation, can enhance production. data indicate triggers premetamorphic B. germanica possibly other hemimetabolan insects.—Kamsoi, O., Belles, X. Myoglianin premetamorphosis stage insects. FASEB J. 33, 3659–3669 (2019). www.fasebj.org

Language: Английский

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RNA interference‐mediated knockdown of the transcription factor Krüppel homologue 1 suppresses vitellogenesis in Chilo suppressalis

Insect Molecular Biology, Journal Year: 2019, Volume and Issue: 29(2), P. 183 - 192

Published: Oct. 1, 2019

Abstract Vitellogenesis in holometabolous insects involves the production and secretion of vitellogenin (Vg) other yolk protein precursors developing oocyte by fat body, all which is predominantly orchestrated juvenile hormone (JH). Krüppel homologue 1 ( Kr‐h1 ) a zinc finger transcription factor that has been demonstrated to be JH‐early inducible gene contribute reproduction. However, exact molecular function insect reproduction poorly understood. In current study, we used notorious pest Chilo suppressalis as model system investigate role female Cloning sequencing C. revealed it shares high identity with its homologues from lepidopteran insects. Moreover, RNA interference‐mediated knockdown CsKr‐h1 substantially reduced Vg dramatically decreased deposition also impaired maturation ovarian development, indicating indispensable for normal vitellogenesis . Based on these results, conclude crucial targeting this could potentially new way suppress rice pests.

Language: Английский

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