Promising influences ofScutellaria baicalensisand its two active constituents, baicalin, and baicalein, against metabolic syndrome: A review

Phytotherapy Research, Journal Year: 2021, Volume and Issue: 35(7), P. 3558 - 3574

Published: Feb. 16, 2021

Metabolic syndrome is known as a group of metabolic abnormalities with features including central obesity, insulin resistance, hypercholesterolemia, hypertriglyceridemia, and hypertension well low level high-density lipoprotein (HDL)-cholesterol. Previous studies showed the ameliorating effects Scutellaria baicalensis on parameters, antidiabetic, anti-hyperlipidemic, anti-obesity, antihypertensive. In this review, we deeply mechanistically evaluated different effect S. its two major bioactive constituents, baicalin, baicalein, critical components syndrome, diabetes, hyperlipidemia, hypertension, atherosclerosis. Scientific databases, PubMed, Scopus, Google Scholar were searched in English language until end June 2020. Accordingly, baicalensis, baicalin represent promising control related disorders such atherosclerosis, their following complications. summary, our findings show that active by activation upregulation AMPK PPAR-γ main signaling hemostasis glucose lipid metabolisms may be favorable candidates for prevention treatment syndrome.

Language: Английский

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Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo

Biomedicine & Pharmacotherapy, Journal Year: 2020, Volume and Issue: 131, P. 110673 - 110673

Published: Sept. 1, 2020

Chlorogenic acid, an important active component of coffee with anti-tumor activities, has been found for a hundred years. However, the lack understanding about its target proteins greatly limits exploration molecular mechanisms and clinical applications. Here, in vitro animal experiments showed that chlorogenic acid had significant inhibitory effect on proliferation A549 cells. The ability to naturally emit fluorescence was exploited screen while avoiding false positives brought by chemical modifications when using fluorescent tags. Consequently, we identified verified annexin A2 as covalent binding We also discovered inhibits p50 subunit thereby inhibiting expression downstream anti-apoptotic genes cIAP1 cIAP2 NF-κB signaling pathway cells vivo. Moreover, hindered actin possibly causing inhibition tumor cell cycle migration. Thus, this work demonstrates binds A2, decrease genes, vivo vitro.

Language: Английский

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Application of omics- and multi-omics-based techniques for natural product target discovery

Biomedicine & Pharmacotherapy, Journal Year: 2021, Volume and Issue: 141, P. 111833 - 111833

Published: June 25, 2021

Natural products continue to be an unparalleled source of pharmacologically active lead compounds because their unprecedented structures and unique biological activities. product target discovery is a vital component natural product-based medicine translation development required understand potentially reduce mechanisms that may associated with off-target side effects toxicity. Omics-based techniques, including genomics, transcriptomics, proteomics, metabolomics, bioinformatics, have become recognized as effective tools needed construct innovative strategies discover targets. Although considerable progress has been made, the successful targets remains challenging time-consuming process come increasingly rely on integration multi-omics-based technologies create emerging panomics (a.k.a., integrative omics, pan-omics, multiomics)-based strategies. This review summarizes series studies regarding application omics-based methods in discovery. The advantages disadvantages each technique are discussed, particular focus systematic multi-omics Further, micro-scale single-cell-based techniques introduced, especially deal minute samples.

Language: Английский

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Targeting cathepsin B by cycloastragenol enhances antitumor immunity of CD8 T cells via inhibiting MHC-I degradation

Journal for ImmunoTherapy of Cancer, Journal Year: 2022, Volume and Issue: 10(10), P. e004874 - e004874

Published: Oct. 1, 2022

Background The loss of tumor antigens and depletion CD8 T cells caused by the PD-1/PD-L1 pathway are important factors for immune escape. In recent years, there has been increasing research on traditional Chinese medicine in treatment. Cycloastragenol (CAG), an effective active molecule Astragalus membranaceus , found to have antiviral, anti-aging, anti-inflammatory, other functions. However, its antitumor effect mechanism not clear. Methods CAG was investigated MC38 CT26 mouse transplanted models. further analyzed via single-cell multiomics sequencing. Target responsive accessibility profiling technology used find target protein CAG. Subsequently, explored using confocal microscopy, coimmunoprecipitation transfection mutant plasmids. Finally, combined PD-1 antibodies mice or organoids were investigated. Results We that effectively inhibited growth vivo. Our atlas demonstrated promoted presentation cell-surface characterized enhanced killing function + cells. Mechanistically, bound cathepsin B, which then lysosomal degradation major histocompatibility complex I (MHC-I) aggregation MHC-I cell membrane, boosting antigen. Meanwhile, combination with antibody ability xenograft colorectal cancer organoids. Conclusion data reported first time B downregulation confers immunity explicates natural product

Language: Английский

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Role of baicalin as a potential therapeutic agent in hepatobiliary and gastrointestinal disorders: A review

World Journal of Gastroenterology, Journal Year: 2022, Volume and Issue: 28(26), P. 3047 - 3062

Published: July 7, 2022

Baicalin is a natural bioactive compound derived from Scutellaria baicalensis, which extensively used in traditional Chinese medicine. A literature survey demonstrated the broad spectrum of health benefits baicalin such as antioxidant, anticancer, anti-inflammatory, antimicrobial, cardio-protective, hepatoprotective, renal protective, and neuroprotective properties. hydrolyzed to its metabolite baicalein by action gut microbiota, further reconverted via phase 2 metabolism liver. Many studies have suggested that exhibits therapeutic potential against several types hepatic disorders including fibrosis, xenobiotic-induced liver injury, fatty disease, viral hepatitis, cholestasis, ulcerative colitis, hepatocellular colorectal cancer. During vitro vivo examinations, it has been observed showed protective role gut-associated abnormalities modifying signaling pathways nuclear factor-kappa B, transforming growth factor beta 1/SMAD3, sirtuin 1, p38/mitogen-activated protein kinase/Janus kinase, calcium/calmodulin-dependent kinase kinaseβ/adenosine monophosphate-activated kinase/acetyl-coenzyme carboxylase pathways. Furthermore, also regulates expression fibrotic genes smooth muscle actin, connective tissue factor, β-catenin, inflammatory cytokines interferon gamma, interleukin-6 (IL-6), tumor necrosis factor-alpha, IL-1β, attenuates production apoptotic proteins caspase-3, caspase-9 B-cell lymphoma 2. However, due low solubility poor bioavailability, widespread applications still remain challenge. This review summarized gastrointestinal attributes with an emphasis on molecular mechanisms regulate interaction microbiota.

Language: Английский

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