The insecticide permethrin induces transgenerational behavioral changes linked to transcriptomic and epigenetic alterations in zebrafish (Danio rerio)

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 779, P. 146404 - 146404

Published: March 18, 2021

The pyrethroid insecticide permethrin is widely used for agricultural and domestic purposes. Previous data indicated that it acts as a developmental neurotoxicant can induce transgenerational effects in non-target organisms. However, associated underlying mechanisms remain unclear. aim of this study was to investigate permethrin-related the zebrafish model, identify possible molecular inheritance. Zebrafish (F0) were exposed during early-life (2 h post-fertilization up 28 days). F1 F2 offspring generations obtained by pairing F0 males females, bred unexposed. Locomotor anxiety behavior investigated, together with transcriptomic epigenomic (DNA methylation) changes brains. Permethrin fish hypoactive at adulthood, while from showed specific decrease anxiety-like behavior. In F0, enrichment pathways related glutamatergic synapse activity, which may partly underlie behavioral effects. males, dysregulation similar observed, including subset differentially methylated regions inherited generation stable signaling. Altogether, present results provide novel evidence on neurotoxic permethrin, well mechanistic insight: transient exposure induces persistent transcriptional DNA methylation translate into alteration signaling and, thus, alterations.

Language: Английский

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Twenty years of transcriptomics, 17alpha-ethinylestradiol, and fish

General and Comparative Endocrinology, Journal Year: 2019, Volume and Issue: 286, P. 113325 - 113325

Published: Nov. 13, 2019

Language: Английский

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Toxicological assessment of seven unregulated drinking water Disinfection By-products (DBPs) using the zebrafish embryo bioassay

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 742, P. 140522 - 140522

Published: June 25, 2020

Language: Английский

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Molecular and behavioral responses of zebrafish embryos/larvae after sertraline exposure

Ecotoxicology and Environmental Safety, Journal Year: 2020, Volume and Issue: 208, P. 111700 - 111700

Published: Nov. 23, 2020

Sertraline (SER) is one of the most frequently detected antidepressant drugs in aquatic environments. However, knowledge regarding SER-induced behavioral alterations fish insufficient, as well mechanisms underlying toxicity. The present study aimed to determine and molecular responses larval following SER exposure with a focus on its mode action. Zebrafish embryos (~6 h-post-fertilization, hpf) were exposed three concentrations (1, 10, 100 μg/L) for 6 days, respectively. Evaluated parameters included development, behavior, transcripts related serotonin signaling, levels, acetylcholinesterase activity. Accelerated hatching zebrafish was observed those μg/L at 54 hpf. Locomotor activity (e.g. distance moved mobile cumulative duration) significantly reduced 10 SER. Conversely, showed increased dark-avoidance after 1–100 Of measured only transporter (serta) receptor 2c (5-ht2c) mRNA levels response treatment. unaltered larvae There no differences among groups any concentration tested. Taking together, results evidenced that alters early-staged zebrafish, which may be abnormal expression 5-ht2c. This elucidates characterizes targets sensitive pharmaceuticals fish.

Language: Английский

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Time-, dose- and transgenerational effects of fluoxetine on the behavioural responses of zebrafish to a conspecific alarm substance

Environmental Pollution, Journal Year: 2020, Volume and Issue: 270, P. 116164 - 116164

Published: Nov. 27, 2020

Language: Английский

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