Event-triggered STED imaging

Nature Methods, Journal Year: 2022, Volume and Issue: 19(10), P. 1268 - 1275

Published: Sept. 8, 2022

Monitoring the proteins and lipids that mediate all cellular processes requires imaging methods with increased spatial temporal resolution. STED (stimulated emission depletion) nanoscopy enables fast of nanoscale structures in living cells but is limited by photobleaching. Here, we present event-triggered STED, an automated multiscale method capable rapidly initiating two-dimensional (2D) 3D after detecting events such as protein recruitment, vesicle trafficking second messengers activity using biosensors. applied vicinity detected to maximize We imaged synaptic dynamics at up 24 Hz, 40 ms local calcium activity; endocytosis exocytosis 11 recruitment or pH changes; interaction between endosomal vesicles 3 70 approaching one another. Event-triggered extends capabilities live imaging, enabling novel biological observations real time.

Language: Английский

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MicroRNA Alteration, Application as Biomarkers, and Therapeutic Approaches in Neurodegenerative Diseases

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(9), P. 4718 - 4718

Published: April 25, 2022

MicroRNAs (miRNAs) are essential post-transcriptional gene regulators involved in various neuronal and non-neuronal cell functions play a key role pathological conditions. Numerous studies have demonstrated that miRNAs dysregulated major neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s multiple sclerosis, amyotrophic lateral or Huntington’s disease. Hence, the present work, we constructed comprehensive overview of individual microRNA alterations models above diseases. We also provided evidence promising biomarkers for prognostic diagnostic approaches. In addition, summarized data from literature about miRNA-based therapeutic applications via inhibiting promoting miRNA expression. finally identified overlapping signature across including miR-128, miR-140-5p, miR-206, miR-326, miR-155, associated with etiological cellular mechanisms. However, it remains to be established whether what extent therapies could safely exploited future effective symptomatic disease-modifying approaches different human disorders.

Language: Английский

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Non-coding RNA in the wiring and remodeling of neural circuits

Neuron, Journal Year: 2023, Volume and Issue: 111(14), P. 2140 - 2154

Published: May 24, 2023

Language: Английский

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Brain exposure to SARS-CoV-2 virions perturbs synaptic homeostasis

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(5), P. 1189 - 1206

Published: March 28, 2024

Language: Английский

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The role of synaptic microRNAs in Alzheimer's disease

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2020, Volume and Issue: 1866(12), P. 165937 - 165937

Published: Aug. 20, 2020

Language: Английский

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microRNAs as Early Biomarkers of Alzheimer’s Disease: A Synaptic Perspective

Cells, Journal Year: 2021, Volume and Issue: 10(1), P. 113 - 113

Published: Jan. 9, 2021

Pathogenic processes underlying Alzheimer's disease (AD) affect synaptic function from initial asymptomatic stages, long time before the onset of cognitive decline and neurodegeneration. Therefore, reliable biomarkers enabling early AD diagnosis prognosis are needed to maximize window for therapeutic interventions. MicroRNAs (miRNAs) have recently emerged as promising cost-effective non-invasive AD, since they can be readily detected in different biofluids, including cerebrospinal fluid (CSF) blood. Moreover, a growing body evidence indicates that miRNAs regulate homeostasis plasticity processes, suggesting may involved dysfunction during AD. Here, we review current literature supporting role deficits recent studies evaluating their potential biomarkers. Besides targeting genes related Aβ tau metabolism, several also synaptic-related proteins transcription factors implicated Furthermore, individual molecular signatures been found distinguish between prodromal healthy controls. Overall, these highlight relevance considering stages. However, further validation large cohorts, longitudinal studies, well implementation standardized protocols, establish miRNA-based diagnostic prognostic tools.

Language: Английский

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Anodal and cathodal tDCS modulate neural activity and selectively affect GABA and glutamate syntheses in the visual cortex of cats

The Journal of Physiology, Journal Year: 2020, Volume and Issue: 598(17), P. 3727 - 3745

Published: June 7, 2020

The present study showed that anodal and cathodal transcranial direct current stimulation (tDCS) can respectively increase decrease the amplitude of visually evoked field potentials in stimulated visual cortex cats, with effect lasting for ∼60-70 min. We directly measured tDCS-induced changes concentration inhibitory excitatory neurotransmitters using enzyme-linked immunosorbent assay method tDCS selectively GABA glutamate cortical area. Anodal inhibit synthesis by suppressing expression GABA- glutamate-synthesizing enzymes, respectively.Transcranial evokes long-lasting neuronal excitability target brain region. underlying neural mechanisms remain poorly understood. examined alterations activities, as well (GLU), area 21a (A21a) cat cortex. Our analysis enhanced suppressed activities A21a, indicated a significantly increased decreased (VEPs). lasted By contrast, sham had no significant impact on VEPs A21a. On other hand, GABA, but not GLU, A21a after relative to tDCS, whereas tDCS. Furthermore, GABA-synthesizing enzymes GAD65 GAD67 terms both mRNA protein concentrations GLU-synthesizing enzyme glutaminase (GLS) did change GLS those GAD65/GAD67 Taken together, these results indicate may reduce GLU syntheses thus enhance suppress

Language: Английский

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Emerging role of microRNAs in major depressive disorder and its implication on diagnosis and therapeutic response

Journal of Affective Disorders, Journal Year: 2021, Volume and Issue: 286, P. 80 - 86

Published: March 5, 2021

Language: Английский

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miR‐124‐dependent tagging of synapses by synaptopodin enables input‐specific homeostatic plasticity

The EMBO Journal, Journal Year: 2022, Volume and Issue: 41(20)

Published: July 25, 2022

Language: Английский

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MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior

eLife, Journal Year: 2022, Volume and Issue: 11

Published: May 6, 2022

MDGA molecules can bind neuroligins and interfere with trans-synaptic interactions to neurexins, thereby impairing synapse development. However, the subcellular localization dynamics of MDGAs, or their specific action mode in neurons remain unclear. Here, surface immunostaining endogenous MDGAs single molecule tracking recombinant dissociated hippocampal reveal that are homogeneously distributed exhibit fast membrane diffusion, a small reduction mobility across neuronal maturation. Knocking-down/out using shRNAs CRISPR/Cas9 strategies increases density excitatory synapses, confinement neuroligin-1, phosphotyrosine level associated post-synaptic differentiation. Finally, silencing reduces AMPA receptors, frequency miniature EPSCs (but not IPSCs), selectively enhances evoked AMPA-receptor-mediated CA1 pyramidal neurons. Overall, our results support mechanism by which between neuroligin-1 delays assembly functional synapses containing receptors.

Language: Английский

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