Reactive intermediates for interactome mapping

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 50(5), P. 2911 - 2926

Published: Jan. 1, 2021

The interactions of biomolecules underpin all cellular processes, and the understanding their dynamic interplay can lead to significant advances in treatment disease through identification novel therapeutic strategies. Protein-protein (PPIs) particular play a vital role within this arena, providing basis for majority signalling pathways. Despite great importance, elucidation weak or transient PPIs that cannot be identified by immunoprecipitation remains challenge, particularly relevant environment. Recent approaches towards goal have utilized situ generation high energy intermediates cross-link with neighboring proteins, snapshot biomolecular makeup local area microenvironment, termed interactome. In tutorial review, we discuss these reactive intermediates, how they are generated, impact had on discovery new biology. Broadly, believe strategy has potential significantly accelerate our affect physiology.

Language: Английский

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Dysregulation of organelle membrane contact sites in neurological diseases

Neuron, Journal Year: 2022, Volume and Issue: 110(15), P. 2386 - 2408

Published: May 12, 2022

Language: Английский

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Proximity Labeling in Plants

Annual Review of Plant Biology, Journal Year: 2023, Volume and Issue: 74(1), P. 285 - 312

Published: March 1, 2023

Proteins are workhorses in the cell; they form stable and more often dynamic, transient protein-protein interactions, assemblies, networks have an intimate interplay with DNA RNA. These network interactions underlie fundamental biological processes play essential roles cellular function. The proximity-dependent biotinylation labeling approach combined mass spectrometry (PL-MS) has recently emerged as a powerful technique to dissect complex at molecular level. In PL-MS, by fusing genetically encoded proximity-labeling (PL) enzyme protein or localization signal peptide, is targeted of interest organelle, allowing proximity proteins within zoom radius. biotinylated can then be captured streptavidin beads identified quantified spectrometry. Recently engineered PL enzymes such TurboID much-improved enzymatic activity, enabling spatiotemporal mapping dramatically increased signal-to-noise ratio. PL-MS revolutionized way we perform proteomics overcoming several hurdles imposed traditional technology, biochemical fractionation affinity purification this review, focus on biotin ligase-based applications that been, likely be, adopted plant field. We discuss experimental designs review different choices for ligases, enrichment, quantification strategies. Lastly, validation future perspectives.

Language: Английский

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Metabolic sensing and control in mitochondria

Molecular Cell, Journal Year: 2023, Volume and Issue: 83(6), P. 877 - 889

Published: March 1, 2023

Language: Английский

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Mitochondrial proteome research: the road ahead

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 25(1), P. 65 - 82

Published: Sept. 29, 2023

Language: Английский

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