Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 30, 2024
Hydrogels
containing
chondrocytes
have
exhibited
excellent
potential
in
regenerating
hyaline
cartilage.
However,
are
vulnerable
to
dedifferentiation
during
vitro
culture,
leading
fibrosis
and
mechanical
degradation
of
newly
formed
It
is
proposed
modulate
cartilage
formation
via
the
developed
chondrocyte
pericellular
matrix
(PCM)
-like
scaffolds
for
first
time,
which
S,
M,
L-sized
fabricated
by
femtosecond
laser
maskless
optical
projection
lithography
(FL-MOPL)
bovine
serum
albumin-glyceryl
methacrylate
hydrogel.
Chondrocytes
on
M
PCM-like
scaffold
can
maintain
round
morphology
synthesize
extracellular
(ECM)
induce
regeneration
microtissues
geometrical
restriction.
A
series
with
different
stiffness
those
a
high
Young's
modulus
more
effective
maintaining
phenotype.
The
modulating
influenced
pore
size,
depth,
stiffness,
will
pave
way
better
understanding
geometric
cues
mechanotransduction
interactions
regulating
cell
fate
open
up
new
avenues
tissue
engineering.
Biomolecules,
Journal Year:
2022,
Volume and Issue:
12(3), P. 477 - 477
Published: March 21, 2022
Epidemiological
data
suggest
that
various
noncommunicable
diseases
develop
as
a
result
of
altered
maternal
metabolic
and
physiological
status
due
to
exposure
several
adverse
factors
during
pregnancy.
However,
evidence
for
intrauterine
mechanisms
underlying
the
origin
early
cartilage
disease
in
chronic
osteoarthritic
is
still
lacking.
In
this
study,
we
found
persistent
overnutrition
pregnancy
obese
mothers
led
damage
neonatal
male
mice.
This
was
mainly
characterized
by
increased
apoptosis
with
decreased
expression
chondrocyte
collagen
II
low
Runx
family
transcription
factor
2
(RUNX2)
SRY-box
9
(SOX9).
reduction
also
be
associated
high
leptin
newborn
mice
offspring.
Furthermore,
administration
mitogen-activated
protein
kinase
(MAPK)-extracellular
signal-regulated
(ERK)
inhibitors
primary
chondrocytes
showed
mediated
MAPK/ERK
signaling
activation
thus
affected
key
regulators
matrix
metallopeptidase
1
(MMP1)
tissue
inhibitor
metalloproteinase
(TIMP1),
thereby
altering
mouse
cartilage.
Altogether,
study
provided
insights
into
molecular
cartilage-related
development
new
clues
fetogenetic
diseases.
Cell stem cell,
Journal Year:
2023,
Volume and Issue:
30(9), P. 1179 - 1198.e7
Published: Sept. 1, 2023
Osteoarthritis
is
a
degenerative
joint
disease
that
causes
pain,
degradation,
and
dysfunction.
Excessive
canonical
Wnt
signaling
in
osteoarthritis
contributes
to
chondrocyte
phenotypic
instability
loss
of
cartilage
homeostasis;
however,
the
regulatory
niche
unknown.
Using
temporomandibular
as
model
multiple
species,
we
identify
Lgr5-expressing
secretory
cells
forming
inhibitory
instruct
Wnt-inactive
chondroprogenitors
form
nascent
synovial
regulate
lineage
identity.
Lgr5
ablation
or
suppression
during
development,
aging,
results
depletion
surge
Wnt-activated,
phenotypically
unstable
chondrocytes
with
osteoblast-like
properties.
We
recapitulate
create
StemJEL,
an
injectable
hydrogel
therapy
combining
hyaluronic
acid
sclerostin.
Local
delivery
StemJEL
post-traumatic
osteoarthritic
jaw
knee
joints
rabbit,
rat,
mini-pig
models
restores
homeostasis,
identity,
function.
provide
proof
principal
preserves
alleviates
osteoarthritis.
EMBO Reports,
Journal Year:
2024,
Volume and Issue:
25(4), P. 1773 - 1791
Published: Feb. 26, 2024
Abstract
Skeletal
growth
promoted
by
endochondral
ossification
is
tightly
coordinated
self-renewal
and
differentiation
of
chondrogenic
progenitors.
Emerging
evidence
has
shown
that
multiple
skeletal
stem
cells
(SSCs)
participate
in
cartilage
formation.
However,
as
yet,
no
study
reported
the
existence
common
long-lasting
progenitors
various
types
cartilage.
Here,
we
identify
Gli1
+
(Gli1
CPs),
which
are
distinct
from
PTHrP
or
FoxA2
SSCs,
responsible
for
lifelong
generation
chondrocytes
plate,
vertebrae,
ribs,
other
The
absence
CPs
leads
to
defects
dwarfishness
phenotype
mice.
Furthermore,
show
BMP
signal
plays
an
important
role
maintenance
CPs.
Deletion
Bmpr1α
triggers
quiescence
exit
causes
exhaustion
CPs,
consequently
disrupting
columnar
Collectively,
our
data
demonstrate
long-term
essential
maintain
homeostasis.
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 30, 2024
Hydrogels
containing
chondrocytes
have
exhibited
excellent
potential
in
regenerating
hyaline
cartilage.
However,
are
vulnerable
to
dedifferentiation
during
vitro
culture,
leading
fibrosis
and
mechanical
degradation
of
newly
formed
It
is
proposed
modulate
cartilage
formation
via
the
developed
chondrocyte
pericellular
matrix
(PCM)
-like
scaffolds
for
first
time,
which
S,
M,
L-sized
fabricated
by
femtosecond
laser
maskless
optical
projection
lithography
(FL-MOPL)
bovine
serum
albumin-glyceryl
methacrylate
hydrogel.
Chondrocytes
on
M
PCM-like
scaffold
can
maintain
round
morphology
synthesize
extracellular
(ECM)
induce
regeneration
microtissues
geometrical
restriction.
A
series
with
different
stiffness
those
a
high
Young's
modulus
more
effective
maintaining
phenotype.
The
modulating
influenced
pore
size,
depth,
stiffness,
will
pave
way
better
understanding
geometric
cues
mechanotransduction
interactions
regulating
cell
fate
open
up
new
avenues
tissue
engineering.
