mRNA-based vaccines and therapeutics: an in-depth survey of current and upcoming clinical applications DOI Creative Commons
Yu‐Shiuan Wang,

Monika Kumari,

Guanhong Chen

et al.

Journal of Biomedical Science, Journal Year: 2023, Volume and Issue: 30(1)

Published: Oct. 7, 2023

mRNA-based drugs have tremendous potential as clinical treatments, however, a major challenge in realizing this drug class will promise to develop methods for safely delivering the bioactive agents with high efficiency and without activating immune system. With regard mRNA vaccines, researchers modified structure enhance its stability promote systemic tolerance of antigenic presentation non-inflammatory contexts. Still, delivery naked mRNAs is inefficient results low levels antigen protein production. As such, lipid nanoparticles been utilized improve protect cargo from extracellular degradation. This advance was milestone development vaccines dispelled skepticism about technology yield clinically approved medicines. Following resounding success COVID-19, many other proposed treatment variety diseases. review begins discussion modifications vehicles, well factors that influence administration routes. Then, we summarize applications discuss further key points pertaining preclinical targeting wide range Finally, latest market trends future drugs.

Language: Английский

Lipid nanoparticles (LNPs) for in vivo RNA delivery and their breakthrough technology for future applications DOI
Michaela Jeong, Yeji Lee, Jeongeun Park

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 200, P. 114990 - 114990

Published: July 7, 2023

Language: Английский

Citations

95
Understanding Nanomaterial–Liver Interactions to Facilitate the Development of Safer Nanoapplications DOI
Jiulong Li, Chunying Chen, Tian Xia

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(11)

Published: Jan. 14, 2022

Nanomaterials (NMs) are widely used in commercial and medical products, such as cosmetics, vaccines, drug carriers. Exposure to NMs via various routes dermal, inhalation, ingestion has been shown gain access the systemic circulation, resulting accumulation of liver. The unique organ structures blood flow features facilitate liver sequestration NMs, which may cause adverse effects Currently, most vivo studies focused on at level evaluation gross changes structure functions, however, cell-type-specific uptake responses, well molecular mechanisms cellular levels leading lagging. Herein, authors systematically review diverse interactions with liver, specifically major cell types including Kupffer cells (KCs), sinusoidal endothelial (LSECs), hepatic stellate (HSCs), hepatocytes detailed involved. In addition, knowledge gained nano-liver that can development safer nanoproducts nanomedicine is also reviewed.

Language: Английский

Citations

93
Carrier strategies boost the application of CRISPR/Cas system in gene therapy DOI Creative Commons
Zunkai Xu, Qingnan Wang,

Haiping Zhong

et al.

Exploration, Journal Year: 2022, Volume and Issue: 2(2)

Published: March 15, 2022

Abstract Emerging clustered regularly interspaced short palindromic repeat/associated protein (CRISPR/Cas) genome editing technology shows great potential in gene therapy. However, proteins and nucleic acids suffer from enzymatic degradation the physiological environment low permeability into cells. Exploiting carriers to protect CRISPR system degradation, enhance its targeting of specific tissues cells, reduce immunogenicity is essential stimulate clinical applications. Here, authors review state‐of‐the‐art delivery systems their applications, describe strategies improve safety efficacy mediated editing, categorized by three types cargo formats, that is, Cas: single‐guide RNA ribonucleoprotein, Cas mRNA RNA, plasmid expressing CRISPR/Cas systems. The hope this will help develop safe efficient nanomaterial‐based for tools.

Language: Английский

Citations

80
The 60-year evolution of lipid nanoparticles for nucleic acid delivery DOI
Pieter R. Cullis, Philip L. Felgner

Nature Reviews Drug Discovery, Journal Year: 2024, Volume and Issue: 23(9), P. 709 - 722

Published: July 4, 2024

Language: Английский

Citations

78
mRNA-based vaccines and therapeutics: an in-depth survey of current and upcoming clinical applications DOI Creative Commons
Yu‐Shiuan Wang,

Monika Kumari,

Guanhong Chen

et al.

Journal of Biomedical Science, Journal Year: 2023, Volume and Issue: 30(1)

Published: Oct. 7, 2023

mRNA-based drugs have tremendous potential as clinical treatments, however, a major challenge in realizing this drug class will promise to develop methods for safely delivering the bioactive agents with high efficiency and without activating immune system. With regard mRNA vaccines, researchers modified structure enhance its stability promote systemic tolerance of antigenic presentation non-inflammatory contexts. Still, delivery naked mRNAs is inefficient results low levels antigen protein production. As such, lipid nanoparticles been utilized improve protect cargo from extracellular degradation. This advance was milestone development vaccines dispelled skepticism about technology yield clinically approved medicines. Following resounding success COVID-19, many other proposed treatment variety diseases. review begins discussion modifications vehicles, well factors that influence administration routes. Then, we summarize applications discuss further key points pertaining preclinical targeting wide range Finally, latest market trends future drugs.

Language: Английский

Citations

77