Cross-kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Dec. 22, 2022

Abstract The role of rhizosphere microbiota in the resistance tomato plant against soil-borne Fusarium wilt disease (FWD) remains unclear. Here, we showed that FWD incidence was significantly negatively correlated with diversity both bacterial and fungal communities. Using microbiological culturomic approach, selected 205 unique strains to construct different synthetic communities (SynComs), which were inoculated into germ-free seedlings, their roles suppressing monitored using omics approach. Cross-kingdom (fungi bacteria) SynComs most effective than those Fungal or Bacterial alone. This effect underpinned by a combination molecular mechanisms related immunity microbial interactions contributed study provides new insight dynamics pathogen suppression host interactions. Also, formulation manipulation for functional complementation constitute beneficial strategy controlling disease.

Language: Английский

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Phyllosphere Microbiome

Annual Review of Plant Biology, Journal Year: 2023, Volume and Issue: 74(1), P. 539 - 568

Published: March 1, 2023

The aboveground parts of terrestrial plants are colonized by a variety microbes that collectively constitute the phyllosphere microbiota. Decades pioneering work using individual microbes, including commensals and pathogens, have provided foundational knowledge about how adapt to environment their role in providing biological control against pathogens. Recent studies revealed more complete repertoire microbiota across plant taxa respond regulate level composition Importantly, development several gnotobiotic systems is allowing causative mechanistic determine contributions health productivity. New insights into carries out key processes, photosynthesis, biomass accumulation, reproduction, defense biotic abiotic insults, either presence or absence normal could unleash novel plant- microbiota-based technologies improve agriculturally relevant traits crop plants.

Language: Английский

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Co‐evolution within the plant holobiont drives host performance

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(9)

Published: July 20, 2023

Plants interact with a diversity of microorganisms that influence their growth and resilience, they can therefore be considered as ecological entities, namely "plant holobionts," rather than singular organisms. In plant holobiont, the assembly above- belowground microbiota is ruled by host, microbial, environmental factors. Upon microorganism perception, plants activate immune signaling resulting in secretion factors modulate composition. Additionally, metabolic interdependencies antagonism between microbes are driving forces for community assemblies. We argue complex plant-microbe intermicrobial interactions have been selected during evolution may promote survival fitness associated holobionts. As part this process, evolved metabolite-mediated strategies to selectively recruit beneficial microbiota. Some these members show host-adaptation, from which mutualism rapidly arise. also co-evolved antagonistic activities restrict proliferation high pathogenic potential prevent disease development. Co-evolution within holobionts thus ultimately drives performance.

Language: Английский

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Tapping the rhizosphere metabolites for the prebiotic control of soil-borne bacterial wilt disease

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 26, 2023

Prebiotics are compounds that selectively stimulate the growth and activity of beneficial microorganisms. The use prebiotics is a well-established strategy for managing human gut health. This concept can also be extended to plants where plant rhizosphere microbiomes improve nutrient acquisition disease resistance. However, we lack effective strategies choosing metabolites elicit desired impacts on In this study, target tomato (Solanum lycopersicum) suffering from wilt (caused by Ralstonia solanacearum) as source potential prebiotic metabolites. We identify (ribose, lactic acid, xylose, mannose, maltose, gluconolactone, ribitol) exclusively used soil commensal bacteria (not positively correlated with R. but not efficiently pathogen in vitro. Metabolites application 1 µmol g

Language: Английский

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Deep discovery informs difficult deployment in plant microbiome science

Cell, Journal Year: 2023, Volume and Issue: 186(21), P. 4496 - 4513

Published: Oct. 1, 2023

Plant-associated microbiota can extend plant immune system function, improve nutrient acquisition and availability, alleviate abiotic stresses. Thus, naturally beneficial microbial therapeutics are enticing tools to productivity. The basic definition of across species ecosystems, combined with the development reductionist experimental models manipulation phenotypes microbes, has fueled interest in its translation agriculture. However, great majority microbes exhibiting plant-productivity traits lab greenhouse fail field. Therapeutic must reach détente, establishment uneasy homeostasis, system, invade heterogeneous pre-established plant-associated communities, persist a new potentially remodeled community. Environmental conditions alter community structure thus impact engraftment therapeutic microbes. We survey recent breakthroughs, challenges, opportunities translating from

Language: Английский

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Commensal lifestyle regulated by a negative feedback loop between Arabidopsis ROS and the bacterial T2SS

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 11, 2024

Despite the plant health-promoting effects of microbiota, these assemblages also comprise potentially detrimental microbes. How immunity controls its microbiota to promote health under conditions remains largely unknown. We find that commensal bacteria isolated from healthy Arabidopsis plants trigger diverse patterns reactive oxygen species (ROS) production dependent on immune receptors and completely NADPH oxidase RBOHD selectively inhibited specific commensals, notably Xanthomonas L148. Through random mutagenesis, we L148 gspE, encoding a type II secretion system (T2SS) component, is required for damaging rbohD mutant plants. In planta bacterial transcriptomics reveals suppresses most T2SS gene expression including gspE. colonization protected against pathogen, when gspE was by ROS or mutation. Thus, negative feedback loop between tames leaf turns it into microbe beneficial host.

Language: Английский

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Leaf microbiome dysbiosis triggered by T2SS-dependent enzyme secretion from opportunistic Xanthomonas pathogens

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(1), P. 136 - 149

Published: Jan. 3, 2024

In healthy plants, the innate immune system contributes to maintenance of microbiota homoeostasis, while disease can be associated with microbiome perturbation or dysbiosis, and enrichment opportunistic plant pathogens like Xanthomonas. It is currently unclear whether change occurs independently caused by latter. Here we tested if protein export through type-2 secretion (T2SS) Xanthomonas causes dysbiosis in Arabidopsis thaliana immunocompromised plants. We found that strains secrete a cocktail cell wall-degrading enzymes promote growth during infection. Disease severity leaf tissue degradation were increased A. mutants lacking NADPH oxidase RBOHD. Experiments gnotobiotic synthetic bacterial communities wild-type T2SS-mutant revealed virulence composition are controlled T2SS. Overall, compromised plants enrich pathogens, which damage tissues ultimately cause facilitating specific commensal bacteria.

Language: Английский

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Potential synergy of microplastics and nitrogen enrichment on plant holobionts in wetland ecosystems

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 915, P. 170160 - 170160

Published: Jan. 18, 2024

Language: Английский

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Genetic determinants of endophytism in the Arabidopsis root mycobiome

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Dec. 10, 2021

Abstract The roots of Arabidopsis thaliana host diverse fungal communities that affect plant health and disease states. Here, we sequence the genomes 41 isolates representative A. root mycobiota for comparative analysis with other 79 plant-associated fungi. Our analyses indicate members evolved from ancestors lifestyles retain large repertoires cell wall-degrading enzymes (PCWDEs) effector-like small secreted proteins. We identify a set 84 gene families associated endophytism, including genes encoding PCWDEs acting on xylan (family GH10) cellulose AA9). Transcripts these are also part conserved transcriptional program activated by phylogenetically-distant upon contact. Recolonization experiments individual fungi strains detrimental effects in mono-association colonize more aggressively than those beneficial activities, dominate natural samples. Furthermore, show pectin-degrading enzyme family PL1_7 links aggressiveness endophytic colonization to health.

Language: Английский

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Environmental interference of plant−microbe interactions

Plant Cell & Environment, Journal Year: 2022, Volume and Issue: 45(12), P. 3387 - 3398

Published: Oct. 1, 2022

Environmental stresses can compromise the interactions of plants with beneficial microbes. In present review, experimental results showing that negatively affect abundance and/or functionality plant microbes are summarized. It is proposed environmental interference these plant-microbe explained by stress-mediated induction signalling pathways associated defence hormones and reactive oxygen species. These responses recognized to regulate within plants. The direct negative effect on may also contribute regulation mutualisms. posited that, in stress situations, harbour mechanisms maintain mutualistic associations. Beneficial produce effector proteins increase antioxidant levels counteract detrimental effects them. addition, they deliver specific stress-protective assist their hosts mitigate stresses. Our study contributes understanding how highlights why still benefits stressful environments.

Language: Английский

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