Differential interactions determine anisotropies at interfaces of RNA-based biomolecular condensates

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Abstract Biomolecular condensates form via macromolecular phase separation, giving rise to coexisting phases delineated by interfaces. Here, we characterize the structures of interfaces formed separation driven heterotypic interactions in ternary mixtures two types RNA molecules and polyethylene glycol. We find that purine-rich RNAs are scaffolds drive strong interactions. Conversely, pyrimidine-rich defined weaker They function as adsorbents accumulate at wet scaffolds. Our computations predict have different non-random orientational preferences tested these predictions using single-molecule super resolution imaging tracks motions fluorogenic probes bound molecules. Motions parallel interface were found be faster than perpendicular interface. These findings support previous regarding anisotropies

Language: Английский

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The Role of Membrane-Biomolecular Condensate Interactions in Stress

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100810 - 100810

Published: March 1, 2025

Language: Английский

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Differential interactions determine anisotropies at interfaces of RNA-based biomolecular condensates

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 11, 2025

Biomolecular condensates form via macromolecular phase separation. Here, we report results from our characterization of synthetic formed by separation mixtures comprising two types RNA molecules and the biocompatible polymer polyethylene glycol. Purine-rich RNAs are scaffolds that drive heterotypic interactions. Conversely, pyrimidine-rich adsorbents defined weaker They adsorb onto wet interfaces coexisting phases scaffolds. Lattice-based simulations reproduce phenomenology observed in experiments these predict have different non-random orientational preferences at interfaces. Dynamics were probed using single-molecule tracking fluorogenic probes bound to molecules. These revealed dynamical anisotropy whereby motions probe parallel interface faster than perpendicular interface. Taken together, findings broad implications for designing with tunable interfacial properties.

Language: Английский

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Adsorption of RNA to interfaces of biomolecular condensates enables wetting transitions

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 13, 2023

Biomolecular condensates form via spontaneous and driven phase transitions of multivalent proteins nucleic acids. These macromolecules can be organized in spatially inhomogeneous ways that lead to multiple coexisting dense phases with distinct macromolecular interfaces. While considerable attention has focused on the physical driving forces give rise separation from bulk solutions, interactions underlie adsorption wetting remain unclear. Here, we report pyrimidine-rich RNAs function as adsorbents enable cascades include partial complete formed by purine-rich RNAs. Computations show are scaffolds oriented perpendicular condensate interfaces whereas parallel Our results yield heuristics for design synthetic materials based RNA-rich featuring bespoke local microenvironments created interplay between versus adsorbents.

Language: Английский

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Crosstalk Between Phase-Separated Membraneless Condensates and Membrane-Bound Organelles in Cellular Function and Disease

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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