Myeloid Cell Mobilization and Recruitment by Human Mesothelioma in NSG-SGM3 Mice DOI Creative Commons
Vadim V. Shindyapin, Ekaterina O. Gubernatorova,

Е. А. Горшкова

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2135 - 2135

Published: Dec. 23, 2024

Malignant pleural mesothelioma is a neoplasm that often detected late due to nonspecific symptoms. This study utilized NSG-SGM3 mice examine interactions between human-derived reporter cell line (MZT-Luc2-mCherry) and the host’s myeloid compartment. Tumor growth was assessed using optical tomography, while cytokine/chemokine production analyzed via multiplex assay. Histological immunohistochemical analyses validated epithelioid phenotype. In vitro cells secreted factors associated with chemoattraction functions supporting previously reported myeloid-biased secretory this, post-engraftment analysis revealed increased neutrophil-like Ly6G+ populations decreased Ly6C+ inflammatory monocytes in blood of tumor-bearing mice. Significant infiltration observed tumor, CD11b+ were localized primarily tumor periphery. lysates showed levels neutrophil chemoattractants G-CSF, suggesting not role neutrophils progression. novel model provides platform for studying mesothelioma–host interactions, focusing on It may also serve as tool facilitate development new therapeutic strategies targeting cell-mediated mechanisms mesothelioma.

Language: Английский

Myeloid Cell Mobilization and Recruitment by Human Mesothelioma in NSG-SGM3 Mice DOI Creative Commons
Vadim V. Shindyapin, Ekaterina O. Gubernatorova,

Е. А. Горшкова

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2135 - 2135

Published: Dec. 23, 2024

Malignant pleural mesothelioma is a neoplasm that often detected late due to nonspecific symptoms. This study utilized NSG-SGM3 mice examine interactions between human-derived reporter cell line (MZT-Luc2-mCherry) and the host’s myeloid compartment. Tumor growth was assessed using optical tomography, while cytokine/chemokine production analyzed via multiplex assay. Histological immunohistochemical analyses validated epithelioid phenotype. In vitro cells secreted factors associated with chemoattraction functions supporting previously reported myeloid-biased secretory this, post-engraftment analysis revealed increased neutrophil-like Ly6G+ populations decreased Ly6C+ inflammatory monocytes in blood of tumor-bearing mice. Significant infiltration observed tumor, CD11b+ were localized primarily tumor periphery. lysates showed levels neutrophil chemoattractants G-CSF, suggesting not role neutrophils progression. novel model provides platform for studying mesothelioma–host interactions, focusing on It may also serve as tool facilitate development new therapeutic strategies targeting cell-mediated mechanisms mesothelioma.

Language: Английский

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