Single-cell RNA sequencing integrated with bulk RNA sequencing analysis of clock circadian regulator with prognostic and immune microenvironment in thyroid cancer

Translational Oncology, Journal Year: 2025, Volume and Issue: 53, P. 102299 - 102299

Published: Jan. 31, 2025

Language: Английский

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Construction of a circadian rhythm-related gene signature for predicting the prognosis and immune infiltration of breast cancer

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 6, 2025

In this study, we constructed a model based on circadian rhythm associated genes (CRRGs) to predict prognosis and immune infiltration in patients with breast cancer (BC). By using TCGA CGDB databases, conducted comprehensive analysis of gene expression clinicopathological data. Three different machine learning algorithms were used screen out the characteristic BC prognosis. On basis, prediction about was validated. We also evaluated association model's risk score cells checkpoint genes, analyzed prognostic drug sensitivity model. screened 62 DEGs, including 30 upregulated 32 downregulated performed GO KEGG them. The above DEGs included Cox analysis, LASSO regression, Random Forest SVMV-RFE, respectively, then intersection obtain 5 related (SUV39H2, OPN4, RORB, FBXL6 SIAH2). Risk Score each sample calculated according level coefficient Score= (SUV39H2 ×0.0436) + (OPN4 ×1.4270) (RORB ×0.1917) (FBXL6 ×0.3190) (SIAH2 × -0.1984). SUV39H2, RORB positively correlated Score, while SIAH2 negatively Score. five can construct for predicting invasion BC.

Language: Английский

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Kanglexin, a New Anthraquinone Compound, Inhibits Hepatic Fibrosis by Regulating Glutathione Metabolism with Pck1-Mediated Gluconeogenesis

Published: Jan. 1, 2025

Language: Английский

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Circadian disruption by simulated shift work aggravates periodontitis via orchestrating BMAL1 and GSDMD-mediated pyroptosis

International Journal of Oral Science, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 25, 2025

Abstract Approximately 20% to 30% of the global workforce is engaged in shift work. As a significant cause circadian disruption, work closely associated with an increased risk for periodontitis. Nevertheless, how work-related disruption functions periodontitis remains unknown. Herein, we employed simulated model constructed by controlling environmental light-dark cycles and revealed that exacerbated progression experimental RNA sequencing vitro experiments indicated downregulation core protein brain muscle ARNT-like 1 (BMAL1) activation Gasdermin D (GSDMD)-mediated pyroptosis were involved pathogenesis that. Mechanically, BMAL1 regulated GSDMD-mediated suppressing NOD-like receptor 3 (NLRP3) inflammasome signaling through modulating nuclear subfamily group member (NR1D1), inhibiting Gsdmd transcription via directly binding E-box elements its promoter. accelerated progression, whereas downregulated under further aggravated periodontal destruction increasing GSDMD activity. And restoring level recovery SR8278 injection alleviated work-exacerbated lessening pyroptosis. These findings provide new evidence potential interventional targets disruption-accelerated

Language: Английский

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COLEC10: A potential tumor suppressor and prognostic biomarker in hepatocellular carcinoma through modulation of EMT and PI3K-AKT pathways

Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

Abstract Hepatocellular carcinoma (HCC) is a cancer with poor prognosis, underscoring the urgent need for enhanced detection and management. This study aimed to investigate role of Collectin Subfamily Member 10 (COLEC10) in HCC, which was revealed be associated various diseases. Bioinformatics tools, including GEO, cBioPortal, TCGA, were used identify differentially expressed genes. The prognostic significance COLEC10 assessed two patient cohorts, its functional impact on Hep3B SMMC7721 cells evaluated through CCK-8 Transwell assays. underlying mechanisms HCC progression explored using flow cytometry western blot. downregulated poorer overall survival disease progression. potential interaction COLEC10, CCBE1, FCN3 predicted. identified as indicators HCC. Overexpression inhibited proliferation, migration, invasion cells. overexpression induced G0/G1 cell cycle arrest suppressed epithelial–mesenchymal transition (EMT), regulated protein expression Hedgehog pathway phosphorylation key proteins PI3K-AKT pathway. an independent factor regulates EMT, Hedgehog, pathways, providing new ideas targeted therapy

Language: Английский

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