Biomimetics, Journal Year: 2024, Volume and Issue: 9(3), P. 145 - 145
Published: Feb. 27, 2024
In the forefront of ophthalmic innovation, biomimetic 3D printing and bioprinting technologies are redefining patient-specific therapeutic strategies. This critical review systematically evaluates their application spectrum, spanning oculoplastic reconstruction, retinal tissue engineering, corneal transplantation, targeted glaucoma treatments. It highlights intricacies these technologies, including fundamental principles, advanced materials, bioinks that facilitate replication ocular architecture. The synthesis primary studies from 2014 to 2023 provides a rigorous analysis evolution current clinical implications. is unique in its holistic approach, juxtaposing scientific underpinnings with realities, thereby delineating advantages over conventional modalities, identifying translational barriers. elucidates persistent knowledge deficits outlines future research directions. ultimately accentuates imperative for multidisciplinary collaboration enhance integration biotechnologies, culminating paradigm shift towards individualized care.
Language: Английский
Citations
4
Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 14, 2025
ABSTRACT Immune rejection is a major barrier to the successful human embryonic stem cell‐derived retinal pigment epithelial (hESC‐RPE) transplantation for age‐related macular degeneration (AMD). Traditional strategies mitigate immune involve ablating histocompatibility complex (MHC) molecules on hESC‐RPE. An alternative approach checkpoint overexpression, avoiding natural killer (NK) cell‐mediated destruction due MHC‐I deficiency. Our study highlights benefits of PD‐L1 overexpression without requiring MHC gene deletion, which preserved immunosuppressive functions hESC‐RPE NK cells. In Vivo experiments in models showed that PD‐L1‐expressing grafts exhibited significantly higher survival, reduced apoptosis and enhanced visual protection. Single‐cell transcriptomics revealed activation oxidative stress PD‐L1‐overexpressing grafts. PD‐L1's protective role was further evidenced by improved light transduction host photoreceptors. These findings support as promising strategy improve efficiency hESC‐RPE‐based therapy AMD.
Language: Английский
Citations
0Animal Models for the Evaluation of Retinal Stem Cell Therapies
Progress in Retinal and Eye Research, Journal Year: 2025, Volume and Issue: unknown, P. 101356 - 101356
Published: April 1, 2025
Language: Английский
Citations
0Near-infrared fluorescent nanoprobe enables noninvasive, longitudinal monitoring of graft outcome in RPE transplantation
Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12
Published: May 9, 2025
Objectives Retinal pigment epithelium (RPE) cell transplantation holds therapeutic promise for retinal degenerative diseases, but longitudinal monitoring of graft survival and efficacy remains clinically challenging. The aim this study is to develop a simple effective method the quantification RPE immune rejection in vivo . Methods A nanoprobe was developed modified label donor cells, used monitor position intensity fluorescence signal Immunofluorescence staining single-cell RNA sequencing (scRNA-seq) were characterize types showing determine composition microenvironment associated with subretinal transplantation. Results spatial distribution corresponded site transplantation, decreased over time, while extended choroid. Additionally, detected liver spleen during long-term monitoring. Conversely, mice administered immunosuppressive drug cyclosporine A, decrease slower expansion less pronounced. analysis revealed significant temporal increase proportion macrophages nanoprobe-labeled cells following stability cell-penetrating ability enables labeling niches scRNA-seq identified MDK ANXA1 signaling pathway as initiators cascade, which further amplified by macrophage-mediated pro-inflammatory signaling. Conclusion Near-infrared fluorescent nanoprobes represent reliable tracing observation can be evaluate degree rejection. Molecular facilitates characterization dynamic niche landscape donor-host interactions
Language: Английский
Citations
0Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan
Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)
Published: May 14, 2025
Aging is a multifaceted biological process marked by the decline in both mitotic and postmitotic cellular function, often central to development of age-related diseases. In pursuit slowing or even reversing aging process, prominent strategy significant interest calorie restriction (CR), also known as dietary restriction, potential influence drug called rapamycin (RM). Both CR RM have demonstrated capacity extend healthspan lifespan across diverse array species, including yeast, worms, flies, mice. Nevertheless, their individual combined effects on cells, well comparative analysis, remain areas that demand thorough investigation. this study, we employ RNA-sequencing methodologies comprehensively analyze impact CR, RM, combination (CR + RM) gene expression yeast cells. Our analysis uncovers distinctive, overlapping, contrasting patterns regulation, illuminating unique shared RM. Furthermore, transcriptional synergistic interaction validated extending human
Language: Английский
Citations
0From theory to therapy: a bibliometric and visual study of stem cell advancements in age-related macular degeneration
Cytotherapy, Journal Year: 2024, Volume and Issue: 26(6), P. 616 - 631
Published: March 2, 2024
Background aimsHuman pluripotent stem cells, including embryonic cells and induced offer groundbreaking therapeutic potential for degenerative diseases cellular repair. Despite their significance, a comprehensive bibliometric analysis in this field, particularly relation to age-related macular degeneration (AMD), is yet be conducted. This study aims map the foundational emerging areas cell AMD research through analysis.MethodsThis analyzed articles reviews on from 2000 2022, sourced Web of Science Core Collection. We used VOSviewer CiteSpace visualization data pertaining countries, institutions, authors, journals, references key words. Statistical analyses were conducted using R language Microsoft Excel 365.ResultsIn total, 539 publications included, indicating an increase global literature 2022. The USA was leading contributor, with 239 papers highest H-index, also had average citation rate per article (59.82). Notably, 50% top 10 institutions USA, University California system being most productive. Key authors included Masayo Takahashi, Michiko Mandai, Dennis Clegg, Pete J. Coffey, Boris Stanzel, Budd A. Tucker. Investigative Ophthalmology & Visual published majority relevant (n = 27). words like "clinical trial," "stem therapy," "retinal organoid," progenitor cells" predominant.ConclusionsResearch has grown significantly, highlighting need increased cooperation. Current prioritizes relationship between "ipsc," "induced cell," "cell culture," "human cell." As culture safety have advanced, focus shifted prognosis complications post-transplantation, signifying movement labs clinical settings.
Language: Английский
Citations
3Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Oct. 30, 2023
Abstract Aging is a multifaceted biological process marked by the decline in both mitotic and postmitotic cellular function, often central to development of age-related diseases. In pursuit slowing or even reversing aging process, prominent strategy significant interest calorie restriction (CR), also known as dietary restriction, potential influence drug called rapamycin (RM). Both CR RM have demonstrated capacity extend healthspan lifespan across diverse array species, including yeast, worms, flies, mice. Nevertheless, their individual combined effects on cells, well comparative analysis, remain areas that demand thorough investigation. this study, we employ RNA-sequencing methodologies comprehensively analyze impact CR, RM, combination (CR+RM) gene expression yeast cells. Our analysis uncovers distinctive, overlapping, contrasting patterns regulation, illuminating unique shared RM. Most notably, our findings reveal synergistic effect CR+RM extending result validated human This research offers valuable insights into processes presents strategies for enhancing delaying onset These revolutionize approach implementing these interventions under specific conditions within context
Language: Английский
Citations
1Dielectrophoretic characterization of peroxidized retinal pigment epithelial cells as a model of age-related macular degeneration
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: March 21, 2024
Language: Английский
Citations
0Dielectrophoretic characterization of peroxidized retinal pigment epithelial cells as a model of age-related macular degeneration
BMC Ophthalmology, Journal Year: 2024, Volume and Issue: 24(1)
Published: Aug. 13, 2024
Age-related macular degeneration (AMD) is a prevalent ocular pathology affecting mostly the elderly population. AMD characterized by progressive retinal pigment epithelial (RPE) cell degeneration, mainly caused an impaired antioxidative defense. One of therapeutic procedures involves injecting healthy RPE cells into subretinal space, necessitating pure, suspensions. This study aims to electrically characterize demonstrate possibility using simulations separate from mixture healthy/oxidized dielectrophoresis. BPEI-1 rat were exposed hydrogen peroxide create in-vitro cellular model. Cell viability was evaluated various methods, including microscopic imaging, impedance-based real-time analysis, and MTS assay. Healthy oxidized recording their dielectrophoretic spectra, electric parameters (crossover frequency, membrane conductivity permittivity, cytoplasm conductivity) computed. A COMSOL simulation performed on theoretical microfluidic-based separation chip these parameters. Increasing concentration shifted first crossover frequency toward lower values, permittivity progressively increased. These changes attributed peroxidation, as they diminished when measured treated with antioxidant N-acetylcysteine. The in sufficient for efficient cells, demonstrated simulations. demonstrates that dielectrophoresis can be used ones based electrical properties. method could viable approach obtaining suspensions procedures.
Language: Английский
Citations
0Atrophic Macular Degeneration and Stem Cell Therapy: A Clinical Review
Advances in experimental medicine and biology, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Language: Английский
Citations
0